Reviewed, UniProtKB/Swiss-Prot O69289 (CH60_CAMJE)

Names and origin

Protein names

Recommended name:
    60 kDa chaperonin
Alternative name(s):
    Protein Cpn60
    groEL protein

Gene names

Name:	groL
Synonyms:	groEL, mopA
Ordered Locus Names:	Cj1221

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity.
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein refolding Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliH	Q0PBI9	1	EBI-1329536,EBI-1191439
fliN	O32370	1	EBI-1329536,EBI-1191320
fliY	Q0PC74	1	EBI-1329536,EBI-1191111

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	60 kDa chaperonin HAMAP MF_00600		PRO_0000063322
Experimental info
	Sequence conflict	179	1	A → P in CAA73778. Ref.1
	Sequence conflict	383	1	A → T in CAA73778. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O69289-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 4DA80BC64330C41E	FASTA	545	57,971
	10 20 30 40 50 60 MAKEIIFSDE ARNKLYEGVK KLNDAVKVTM GPRGRNVLIQ KSFGAPSITK DGVSVAKEVE 70 80 90 100 110 120 LKDSLENMGA SLVREVASKT ADQAGDGTTT ATVLAHAIFK EGLRNITAGA NPIEVKRGMD 130 140 150 160 170 180 KACEAIVAEL KKLSREVKDK KEIAQVATIS ANSDEKIGNL IADAMEKVGK DGVITVEEAK 190 200 210 220 230 240 SINDELNVVE GMQFDRGYLS PYFITNAEKM TVELSSPYIL LFDKKITNLK DLLPVLEQIQ 250 260 270 280 290 300 KTGKPLLIIA EDIEGEALAT LVVNKLRGVL NISAVKAPGF GDRRKAMLED IAILTGGEVI 310 320 330 340 350 360 SEELGRTLES ATIQDLGQAS SVIIDKDNTT IVNGAGEKAN IDARVNQIKA QIAETTSDYD 370 380 390 400 410 420 REKLQERLAK LSGGVAVIKV GAATETEMKE KKDRVDDALS ATKAAVEEGI VIGGGAALIK 430 440 450 460 470 480 AKAKIKLDLQ GDEAIGAAIV ERALRAPLRQ IAENAGFDAG VVVNSVENAK DENTGFDAAK 490 500 510 520 530 540 GEYVNMLESG IIDPVKVERV ALLNAVSVAS MLLTTEATIS EIKEDKPTMP DMSGMGGMGG MGGMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and molecular analysis of the Campylobacter jejuni groESL bicistronic operon." Thies F.L., Weishaupt A., Karch H., Hartung H.P., Giegerich G. Microbiology 145:89-98(1999) [PubMed: 10206714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the cpn60 gene from various Campylobacter jejuni isolates." Cunningham A., Taboada E., Nash J.H., Wakarchuk W.W., Gilbert M. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43429 / MK5-S7630 / Serotype O:1, ATCC 43432 / MK7 / Serotype O:4, ATCC 43438 / MK1 / Serotype O:10 and ATCC 43456 / MK290 / Serotype O:36.
[3]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13334 Genomic DNA. Translation: CAA73778.1. AF461064 Genomic DNA. Translation: AAL76936.1. AF461534 Genomic DNA. Translation: AAL67841.1. AF461535 Genomic DNA. Translation: AAL67842.1. AF461537 Genomic DNA. Translation: AAL67844.1. AL111168 Genomic DNA. Translation: CAL35336.1.
PIR	G81328.
RefSeq	YP_002344612.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOK based on UniProtKB Q9Z462.
ModBase	Search...
Protein-protein interaction databases
IntAct	O69289. 97 interactions.
PTM databases
PhosSite	O69289.
Genome annotation databases
GeneID	905511.
GenomeReviews	Gene locus Cj1221 in contig AL111168_GR.
KEGG	cje:Cj1221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O69289.
OMA	O69289. KLEMLGT.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1221-MON.
Family and domain databases
HAMAP	MF_00600. [Tree]
InterPro	IPR018370. Chaperonin_Cpn60_CS. IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CH60_CAMJE

Accession

Primary (citable) accession number: O69289
Secondary accession number(s): Q0P935, Q9PN75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)