O69282 (MQO_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate:quinone oxidoreductase
EC=1.1.5.4

Alternative name(s):
MQO
Malate dehydrogenase [quinone]

Gene names

Name:	mqo
Ordered Locus Names:	Cgl2001, cg2192

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-malate + a quinone = oxaloacetate + reduced quinone. HAMAP MF_00212
Cofactor	FAD. The FAD is tightly bound.
Enzyme regulation	Activated by lipids. HAMAP MF_00212
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; oxaloacetate from (S)-malate (quinone route): step 1/1. HAMAP MF_00212
Subcellular location	Cell membrane; Peripheral membrane protein HAMAP MF_00212.
Sequence similarities	Belongs to the MQO family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate dehydrogenase (menaquinone) activity Inferred from electronic annotation. Source: EC malate dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 500	499	Malate:quinone oxidoreductase HAMAP MF_00212		PRO_0000128711

Sequences

	Sequence		Length	Mass (Da)
	O69282 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 31E02BC151758319	FASTA	500	54,832
	10 20 30 40 50 60 MSDSPKNAPR ITDEADVVLI GAGIMSSTLG AMLRQLEPSW TQIVFERLDG PAQESSSPWN 70 80 90 100 110 120 NAGTGHSALC ELNYTPEVKG KVEIAKAVGI NEKFQVSRQF WSHLVEEGVL SDPKEFINPV 130 140 150 160 170 180 PHVSFGQGAD QVAYIKARYE ALKDHPLFQG MTYADDEATF TEKLPLMAKG RDFSDPVAIS 190 200 210 220 230 240 WIDEGTDINY GAQTKQYLDA AEVEGTEIRY GHEVKSIKAD GAKWIVTVKN VHTGDTKTIK 250 260 270 280 290 300 ANFVFVGAGG YALDLLRSAG IPQVKGFAGF PVSGLWLRCT NEELIEQHAA KVYGKASVGA 310 320 330 340 350 360 PPMSVPHLDT RVIEGEKGLL FGPYGGWTPK FLKEGSYLDL FKSIRPDNIP SYLGVAAQEF 370 380 390 400 410 420 DLTKYLVTEV LKDQDKRMDA LREYMPEAQN GDWETIVAGQ RVQVIKPAGF PKFGSLEFGT 430 440 450 460 470 480 TLINNSEGTI AGLLGASPGA SIAPSAMIEL LERCFGDRMI EWGDKLKDMI PSYGKKLASE 490 500 PALFEQQWAR TQKTLKLEEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum." Molenaar D., van der Rest M.E., Petrovic S. Eur. J. Biochem. 254:395-403(1998) [PubMed: 9660197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R127.
[2]	van der Rest M.E. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]	Molenaar D., van der Rest M.E., Petrovic S. Submitted (AUG-1998) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-6. Strain: R127.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224946 Genomic DNA. Translation: CAA12237.1. BA000036 Genomic DNA. Translation: BAB99394.1. BX927153 Genomic DNA. Translation: CAF20342.1.
RefSeq	NP_601207.1. NC_003450.3. YP_226243.1. NC_006958.1.
3D structure databases
ProteinModelPortal	O69282.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:O69282.
Proteomic databases
PRIDE	O69282.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019958. 3345228.
GenomeReviews	Gene locus Cgl2001 in contig BA000036_GR. Gene locus cg2192 in contig BX927147_GR.
KEGG	cgb:cg2192. cgl:NCgl1926.
PATRIC	21496008. VBICorGlu203724_1940.
Phylogenomic databases
HOGENOM	HBG289663.
OMA	LEKEWAY.
PhylomeDB	O69282.
ProtClustDB	PRK05257.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2192-MONOMER.
Family and domain databases
HAMAP	MF_00212. MQO. [Tree]
InterPro	IPR006231. Malate_quinone_OxRdtase. [Graphical view]
KO	K00116.
PANTHER	PTHR13847:SF14. PTHR13847:SF14. 1 hit.
Pfam	PF06039. Mqo. 1 hit. [Graphical view]
TIGRFAMs	TIGR01320. Mal_quin_oxido. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MQO_CORGL

Accession

Primary (citable) accession number: O69282

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program