Ribonucleotide reductase subunit R2F - Corynebacterium ammoniagenes

Preferred order of sections

Names and origin

Protein names

Submitted name:
Ribonucleotide reductase subunit R2F EMBL CAA70766.1

Gene names

Name:

nrdF EMBL CAA70766.1

Organism

Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) EMBL CAA70766.1

Taxonomic identifier

1697 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Iron PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU Metal-binding PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU
Technical term	3D-structure PDB 1KGO PDB 3DHZ PDB 3MJO PDB 1KGP PDB 1KGN PDB 1OQU
Gene Ontology (GO)
Biological process	deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: InterPro transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

77

1

Iron 1 PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

108

1

Iron 1 PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

108

1

Iron 2 PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

111

1

Iron 1; via pros nitrogen PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

168

1

Iron 2 PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

202

1

Iron 1 PDB 1KGO PDB 3DHZ PDB 1OQU

Metal binding

202

1

Iron 2 PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Metal binding

205

1

Iron 2; via pros nitrogen PDB 1KGO PDB 3DHZ PDB 1KGN PDB 1OQU

Sequences

Sequence

Length

Mass (Da)

Tools

O69274 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 328C3357CBBFFFA8
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FASTA

329

37,926

        10         20         30         40         50         60 
MSNEYDEYIA NHTDPVKAIN WNVIPDEKDL EVWDRLTGNF WLPEKIPVSN DIQSWNKMTP 

        70         80         90        100        110        120 
QEQLATMRVF TGLTLLDTIQ GTVGAISLLP DAETMHEEAV YTNIAFMESV HAKSYSNIFM 

       130        140        150        160        170        180 
TLASTPQINE AFRWSEENEN LQRKAKIIMS YYNGDDPLKK KVASTLLESF LFYSGFYLPM 

       190        200        210        220        230        240 
YLSSRAKLTN TADIIRLIIR DESVHGYYIG YKYQQGVKKL SEAEQEEYKA YTFDLMYDLY 

       250        260        270        280        290        300 
ENEIEYTEDI YDDLGWTEDV KRFLRYNANK ALNNLGYEGL FPTDETKVSP AILSSLSPNA 

       310        320 
DENHDFFSGS GSSYVIGKAE DTTDDDWDF

« Hide

References

[1]	"The manganese-containing ribonucleotide reductase of Corynebacterium ammoniagenes is a class Ib enzyme." Fieschi F., Torrents E., Toulokhonova L., Jordan A., Hellman U., Barbe J., Gibert I., Karlsson M., Sjoeberg B.-M. J. Biol. Chem. 273:4329-4337(1998) [PubMed: 9468481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC6872 EMBL CAA70766.1.
[2]	"Crystal structure of the di-iron/radical protein of ribonucleotide reductase from Corynebacterium ammoniagenes." Hogbom M., Huque Y., Sjoberg B.M., Nordlund P. Biochemistry 41:1381-1389(2002) [PubMed: 11802741] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH IRON.
[3]	"A protein carboxylate coordinated oxo-centered tri-nuclear iron complex with possible implications for ferritin mineralization." Hogbom M., Nordlund P. FEBS Lett. 567:179-182(2004) [PubMed: 15178319] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH IRON.
[4]	"A tyrosyl-dimanganese coupled spin system is the native metalloradical cofactor of the R2F subunit of the ribonucleotide reductase of Corynebacterium ammoniagenes." Cox N., Ogata H., Stolle P., Reijerse E., Auling G., Lubitz W. J. Am. Chem. Soc. 132:11197-11213(2010) [PubMed: 20698687] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS) OF 2-297.
[5]	"Structural and mutational studies of the carboxylate cluster in iron-free ribonucleotide reductase R2." Andersson M.E., Hogbom M., Rinaldo-Matthis A., Blodig W., Liang Y., Persson B.O., Sjoberg B.M., Su X.D., Nordlund P. Biochemistry 43:7966-7972(2004) [PubMed: 15196041] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) IN COMPLEX WITH IRON.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09572 Genomic DNA. Translation: CAA70766.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KGN	X-ray	1.85	A/B/C/D	1-329	[»]
1KGO	X-ray	2.25	A/B/C/D	1-329	[»]
1KGP	X-ray	2.00	A/B/C/D	1-329	[»]
1OQU	X-ray	2.00	A/B/C/D	1-329	[»]
3DHZ	X-ray	1.63	A/B	1-329	[»]
3MJO	X-ray	1.36	A/B	2-297	[»]

ProteinModelPortal

O69274.

SMR

O69274. Positions 2-297.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR009078. Ferritin/RR-like.
IPR012348. Ribncl_red-rel.
IPR000358. Ribonucl_redctse.
[Graphical view]

Gene3D

G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.

PANTHER

PTHR23409. Ribonucl_redctse. 1 hit.

Pfam

PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

PROSITE

PS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O69274_CORAM

Accession

Primary (citable) accession number: O69274

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	July 27, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)