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Protein

Diaminopimelate decarboxylase

Gene

lysA

Organism
Actinosynnema pretiosum subsp. auranticum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.UniRule annotation

Catalytic activityi

Meso-2,6-diaminoheptanedioate = L-lysine + CO2.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate decarboxylase (lysA)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei301 – 3011SubstrateUniRule annotation
Binding sitei337 – 3371SubstrateUniRule annotation
Binding sitei341 – 3411SubstrateUniRule annotation
Active sitei366 – 3661Proton donorSequence analysis
Binding sitei367 – 3671SubstrateUniRule annotation
Binding sitei396 – 3961Pyridoxal phosphateUniRule annotation
Binding sitei396 – 3961SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00034; UER00027.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate decarboxylaseUniRule annotation (EC:4.1.1.20UniRule annotation)
Short name:
DAP decarboxylaseUniRule annotation
Short name:
DAPDCUniRule annotation
Gene namesi
Name:lysAUniRule annotation
OrganismiActinosynnema pretiosum subsp. auranticum
Taxonomic identifieri42198 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeActinosynnema

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Diaminopimelate decarboxylasePRO_0000149911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei81 – 811N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PRIDEiO69203.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO69203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3014Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.UniRule annotation

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_02120. LysA. 1 hit.
InterProiIPR009006. Ala_racemase/Decarboxylase_C.
IPR002986. DAP_deCOOHase_LysA.
IPR022643. De-COase2_C.
IPR022644. De-COase2_N.
IPR000183. Orn/DAP/Arg_de-COase.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF02784. Orn_Arg_deC_N. 1 hit.
PF00278. Orn_DAP_Arg_deC. 1 hit.
[Graphical view]
PRINTSiPR01181. DAPDCRBXLASE.
PR01179. ODADCRBXLASE.
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR01048. lysA. 1 hit.

Sequencei

Sequence statusi: Complete.

O69203-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLAELLPSV GMTGEPALET GLWPLGTRLE RGELLLGGVP ATELAARFGT
60 70 80 90 100
PCQVLDEGTV RARARAFREA LPEAEVVFAG KALPCREVYR WVADEGLSLD
110 120 130 140 150
VCSAGELAIA RSVGFPAERV LLHGNVKTPE DLKAALGYGV GRVVVDSFDE
160 170 180 190 200
IEQLGALAEG PQDVLVRVTP GVDPRTHRAV ATGVEDQKFG FSLAGGDALE
210 220 230 240 250
AVLRVVEQPS LRLVGLHCHV GSQVRHVAVY EEAARRVVGL IASCGVRIEQ
260 270 280 290 300
LDLGGGFAVP YLPGEGEFDL GGFAHRVRVA LSHECALRRV PVPRLSIEPG
310 320 330 340 350
RAVVARAGVT LYRVAAVKRG VRRVFVAVDG GMSDNPRPAL YGSRYAVRLV
360 370 380 390 400
RRGGRRAPVT VVGRHCEAGD VLAEDVPLPE DVRAGDLLAV PVTGAYHHAL
410 420 430
ASNYNAVGRP PVVGVRDGVA RVLVRRETEE DLLRREV
Length:437
Mass (Da):46,641
Last modified:August 1, 1998 - v1
Checksum:iDF41D6DECAF15713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33059 Genomic DNA. Translation: AAC13998.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33059 Genomic DNA. Translation: AAC13998.1.

3D structure databases

ProteinModelPortaliO69203.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO69203.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00034; UER00027.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_02120. LysA. 1 hit.
InterProiIPR009006. Ala_racemase/Decarboxylase_C.
IPR002986. DAP_deCOOHase_LysA.
IPR022643. De-COase2_C.
IPR022644. De-COase2_N.
IPR000183. Orn/DAP/Arg_de-COase.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF02784. Orn_Arg_deC_N. 1 hit.
PF00278. Orn_DAP_Arg_deC. 1 hit.
[Graphical view]
PRINTSiPR01181. DAPDCRBXLASE.
PR01179. ODADCRBXLASE.
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR01048. lysA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCDA_ACTPA
AccessioniPrimary (citable) accession number: O69203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.