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O69161 (RNC_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:bll5061
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Sequence caution

The sequence BAC50326.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000180377

Regions

Domain22 – 150129RNase III
Domain175 – 24470DRBM

Sites

Active site671 Potential
Active site1391 By similarity
Metal binding631Magnesium By similarity
Metal binding1361Magnesium By similarity
Metal binding1391Magnesium By similarity

Experimental info

Sequence conflict1 – 3232MTKTP…RIGHS → MRTRRLRRASGHT in AAD02939. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O69161 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: 19E6FE9D2DDDC930

FASTA25026,856
        10         20         30         40         50         60 
MTKTPAKKKR ARSSKAKGTD ANAALEARIG HSFADPNLLM QGITHVSALK SGRKRGDSYQ 

        70         80         90        100        110        120 
RLEFLGDHVL GLVVSDMLYH AFPNADEGEL SKRLAELVRK ESCADVAKSL GLLDDIKLGS 

       130        140        150        160        170        180 
VGPSADARLR KSVLGDICEA VIGAIFLDGG HAAAAEFVKR NWTERMHKPR RPLRDPKTVL 

       190        200        210        220        230        240 
QEWAQGKGLP TPVYREVERT GPHHDPQFRV AVDLPGLAPA EGIGGSKRAA EKVAASVMIE 

       250 
REGVGGGNDG 

« Hide

References

« Hide 'large scale' references
[1]"sipF, the second functional type I signal peptidase of Bradyrhizobium japonicum, is encoded in the vicinity of essential genes involved in RNA processing and cell division."
Bairl A., Mueller P.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF065159 Genomic DNA. Translation: AAD02939.1.
BA000040 Genomic DNA. Translation: BAC50326.1. Different initiation.
RefSeqNP_771701.1. NC_004463.1.

3D structure databases

ProteinModelPortalO69161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll5061.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50326; BAC50326; BAC50326.
GeneID1051815.
KEGGbja:bll5061.
PATRIC21193792. VBIBraJap65052_5141.

Phylogenomic databases

eggNOGCOG0571.
HOGENOMHOG000246809.
KOK03685.
OMALTHKSCK.
OrthoDBEOG6T1WVS.
ProtClustDBCLSK819697.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-5098-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_BRADU
AccessionPrimary (citable) accession number: O69161
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 28, 2003
Last modified: April 16, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families