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O69161

- RNC_BRADU

UniProt

O69161 - RNC_BRADU

Protein

Ribonuclease 3

Gene

rnc

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (28 Feb 2003)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

    Cofactori

    Mg2+.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631MagnesiumUniRule annotation
    Active sitei67 – 671UniRule annotation
    Metal bindingi136 – 1361MagnesiumUniRule annotation
    Active sitei139 – 1391UniRule annotation
    Metal bindingi139 – 1391MagnesiumUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-5098-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
    Alternative name(s):
    Ribonuclease IIIUniRule annotation
    Short name:
    RNase IIIUniRule annotation
    Gene namesi
    Name:rncUniRule annotation
    Ordered Locus Names:bll5061
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250Ribonuclease 3PRO_0000180377Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224911.bll5061.

    Structurei

    3D structure databases

    ProteinModelPortaliO69161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 150129RNase IIIUniRule annotationAdd
    BLAST
    Domaini175 – 24470DRBMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
    Contains 1 RNase III domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0571.
    HOGENOMiHOG000246809.
    KOiK03685.
    OrthoDBiEOG6T1WVS.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O69161-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKTPAKKKR ARSSKAKGTD ANAALEARIG HSFADPNLLM QGITHVSALK    50
    SGRKRGDSYQ RLEFLGDHVL GLVVSDMLYH AFPNADEGEL SKRLAELVRK 100
    ESCADVAKSL GLLDDIKLGS VGPSADARLR KSVLGDICEA VIGAIFLDGG 150
    HAAAAEFVKR NWTERMHKPR RPLRDPKTVL QEWAQGKGLP TPVYREVERT 200
    GPHHDPQFRV AVDLPGLAPA EGIGGSKRAA EKVAASVMIE REGVGGGNDG 250
    Length:250
    Mass (Da):26,856
    Last modified:February 28, 2003 - v2
    Checksum:i19E6FE9D2DDDC930
    GO

    Sequence cautioni

    The sequence BAC50326.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 3232MTKTP…RIGHS → MRTRRLRRASGHT in AAD02939. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF065159 Genomic DNA. Translation: AAD02939.1.
    BA000040 Genomic DNA. Translation: BAC50326.1. Different initiation.
    RefSeqiNP_771701.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC50326; BAC50326; BAC50326.
    GeneIDi1051815.
    KEGGibja:bll5061.
    PATRICi21193792. VBIBraJap65052_5141.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF065159 Genomic DNA. Translation: AAD02939.1 .
    BA000040 Genomic DNA. Translation: BAC50326.1 . Different initiation.
    RefSeqi NP_771701.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali O69161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.bll5061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC50326 ; BAC50326 ; BAC50326 .
    GeneIDi 1051815.
    KEGGi bja:bll5061.
    PATRICi 21193792. VBIBraJap65052_5141.

    Phylogenomic databases

    eggNOGi COG0571.
    HOGENOMi HOG000246809.
    KOi K03685.
    OrthoDBi EOG6T1WVS.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-5098-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "sipF, the second functional type I signal peptidase of Bradyrhizobium japonicum, is encoded in the vicinity of essential genes involved in RNA processing and cell division."
      Bairl A., Mueller P.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: USDA 110spc4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiRNC_BRADU
    AccessioniPrimary (citable) accession number: O69161
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 28, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3