Reviewed, UniProtKB/Swiss-Prot O69161 (RNC_BRAJA)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	bll5061

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Ribonuclease 3 HAMAP MF_00104		PRO_0000180377
Regions
	Domain	22 – 150	129	RNase III
	Domain	175 – 244	70	DRBM
Experimental info
	Sequence conflict	1 – 32	32	MTKTP…RIGHS → MRTRRLRRASGHT Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O69161-1 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 19E6FE9D2DDDC930	FASTA	250	26,856
	10 20 30 40 50 60 MTKTPAKKKR ARSSKAKGTD ANAALEARIG HSFADPNLLM QGITHVSALK SGRKRGDSYQ 70 80 90 100 110 120 RLEFLGDHVL GLVVSDMLYH AFPNADEGEL SKRLAELVRK ESCADVAKSL GLLDDIKLGS 130 140 150 160 170 180 VGPSADARLR KSVLGDICEA VIGAIFLDGG HAAAAEFVKR NWTERMHKPR RPLRDPKTVL 190 200 210 220 230 240 QEWAQGKGLP TPVYREVERT GPHHDPQFRV AVDLPGLAPA EGIGGSKRAA EKVAASVMIE 250 REGVGGGNDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"sipF, the second functional type I signal peptidase of Bradyrhizobium japonicum, is encoded in the vicinity of essential genes involved in RNA processing and cell division." Bairl A., Mueller P. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF065159 Genomic DNA. Translation: AAD02939.1. BA000040 Genomic DNA. Translation: BAC50326.1. Different initiation.
RefSeq	NP_771701.1.
3D structure databases
HSSP	HSSP built from PDB template 1O0W based on UniProtKB Q9X0I6.
ModBase	Search...
Genome annotation databases
GeneID	1051815.
GenomeReviews	Gene locus bll5061 in contig BA000040_GR.
KEGG	bja:bll5061.
NMPDR	fig|224911.1.peg.5061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O69161.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5061-MON.
BRENDA	3.1.26.3. 280.
Family and domain databases
HAMAP	MF_00104. [Tree]
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BRAJA

Accession

Primary (citable) accession number: O69161

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 28, 2003
Last modified:	June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)