Reviewed, UniProtKB/Swiss-Prot O69159 (ACPS_BRAJA)

Names and origin

Protein names

Recommended name:
    Holo-[acyl-carrier-protein] synthase
      Short name=Holo-ACP synthase
    EC=2.7.8.7
Alternative name(s):
    4'-phosphopantetheinyl transferase acpS

Gene names

Name:	acpS
Ordered Locus Names:	bll5063

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity.
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 139	139	Holo-[acyl-carrier-protein] synthase HAMAP MF_00101		PRO_0000175618
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	61	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69159-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2FD4A315845FC788	FASTA	139	15,193
	10 20 30 40 50 60 MIIGIGSDLI DITRVGKVIE RHGERFLDRI FTAAERAKAE RRAKNEKMVV ATYAKRFAAK 70 80 90 100 110 120 EACSKALGTG IRRGVWWRDM GVVNLPGGRP TMQLTGGALA RLQALTPDGF EARIDVSITD 130 DWPLAQAFVI ISAVPLAKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second gene for type I signal peptidase in Bradyrhizobium japonicum, sipF, is located near genes involved in RNA processing and cell division." Bairl A., Mueller P. Mol. Gen. Genet. 260:346-356(1998) [PubMed: 9870699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF065159 Genomic DNA. Translation: AAD02937.1. BA000040 Genomic DNA. Translation: BAC50328.1.
RefSeq	NP_771703.1.
3D structure databases
HSSP	HSSP built from PDB template 1F7L based on UniProtKB P96618.
ModBase	Search...
Genome annotation databases
GeneID	1051811.
GenomeReviews	Gene locus bll5063 in contig BA000040_GR.
KEGG	bja:bll5063.
NMPDR	fig|224911.1.peg.5063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O69159.
OMA	MGVVNLP.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5063-MON.
BRENDA	2.7.8.7. 280.
Family and domain databases
HAMAP	MF_00101. [Tree]
InterPro	IPR008278. 4-PPantetheinyl_Trfase. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase. [Graphical view]
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProDom	PD004282. ACPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00516. acpS. 1 hit. TIGR00556. pantethn_trn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPS_BRAJA

Accession

Primary (citable) accession number: O69159

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)