O69140 (PCL_RHOCB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase
Short name=4CL
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase

Gene names

Name:	pcl
Ordered Locus Names:	RCAP_rcc01063

Organism

Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) [Complete proteome] [HAMAP]

Taxonomic identifier

272942 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore By similarity.
Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAC17429.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	4-coumarate--CoA ligase		PRO_0000193044

Sequences

	Sequence		Length	Mass (Da)
	O69140 [UniParc]. Last modified May 31, 2011. Version 2. Checksum: D9184B69E58A1466	FASTA	384	40,942
	10 20 30 40 50 60 MVRRLLISLI RAEARRGRNQ ILPEAAFTGD PRIDEEGLGF DSLARLDLIG AVRDFFDLSR 70 80 90 100 110 120 TGIEDYVYVE PTLQGWIDRI MQHFDLLAAR SETAQAVFRT SGSTGTPKPI PHPWPKLMRE 130 140 150 160 170 180 AASMARDQGL VPAPGGAVIG LVPAHHLFGC LFTALLPELA GAALRDLTAA PPASALRTAQ 190 200 210 220 230 240 PGDLIIATPH LWAHLGAAGA FPPGLRGVSS GAPMPDALWH SLLAAGLEDL TEVYGASETG 250 260 270 280 290 300 GIGLRRAPGA AFTLLPFLSR SADDGISDGP APLPLQDRLR WTGPVRFVIE GRLDQALQVG 310 320 330 340 350 360 GVNVRLGHVK SVLEAEPGVE ALALRLGGDR LKAFVVCAAD AEAGLEARLR ARAEAGLDAP 370 380 ARPQHYRFGR ALPLTREGKA RDWD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic characterization of photoactive yellow protein from Rhodobacter capsulatus." Jiang Z., Bauer E.C. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[2]	"Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003." Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R. J. Bacteriol. 192:3545-3546(2010) [PubMed: 20418398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF064095 Genomic DNA. Translation: AAC17429.1. Different initiation. CP001312 Genomic DNA. Translation: ADE84823.1.
RefSeq	YP_003577230.1. NC_014034.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9003892.
GenomeReviews	Gene locus RCAP_rcc01063 in contig CP001312_GR.
KEGG	rcp:RCAP_rcc01063.
PATRIC	35502220. VBIRhoCap134200_1081.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR012743. 4_coum_CoA_lig. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02372. 4_coum_CoA_lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCL_RHOCB

Accession

Primary (citable) accession number: O69140
Secondary accession number(s): D5AR17

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 31, 2011
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program