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Protein

Translational regulator CsrA

Gene

csrA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA. Its function is antagonized by small RNA(s).UniRule annotation
Binds to mRNA to regulate gene activity at a post-transcriptional level (Probable). Represses expression of many toxic extracellular enzymes and compounds; decreases translation of lasI and rhlI (PubMed:11673439). Positively controls swarming motility and rhanolipid and lipase, possibly via expression of rhlA; activates transcription of the CsrA/RsmA antagonistic sRNA RsmZ (PubMed:15126453). Overexpression dramatically reduces extracellular protease, elastase (lasB) and staphyolytic (lasA) activities, decreases HCN production, decreases levels of autoinducers 3-oxo-C12-HSL (3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-dodecanamide) and C4-HSL (N-butanoylhomoserine lactone), and abolishes production of cytotoxic internal lectin PA-IL (lecA) (PubMed:11673439). Control of hcn expression is post-transcriptional (PubMed:11673439). Replaces endogenous gene(s) in E.coli and P.fluorescens (PubMed:16359708).3 Publications
Probably binds to and is sequestered by non-coding small RNA (sRNA) RsmZ; overexpression of rsmZ produces very similar phenotypes to deletion of rsmA, while rsmZ deletion has no phenotype (PubMed:15126453).1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA catabolic process Source: InterPro
  • pathogenesis Source: PseudoCAP
  • protein secretion by the type III secretion system Source: PseudoCAP
  • protein secretion by the type VI secretion system Source: PseudoCAP
  • quorum sensing Source: PseudoCAP
  • regulation of carbohydrate metabolic process Source: InterPro
  • regulation of secondary metabolic process Source: PseudoCAP
  • regulation of single-species biofilm formation Source: PseudoCAP
  • regulation of translation Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Repressor, RNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-921-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Translational regulator CsrAUniRule annotation
Alternative name(s):
Carbon storage regulatorUniRule annotation
Global translational regulatory protein RsmA1 Publication
Gene namesi
Name:csrAUniRule annotation
Synonyms:rsmA1 Publication
Ordered Locus Names:PA0905
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0905

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Grows slightly more slowly; 30% reduction in staphylolytic activity, increased production of lectin PA-IL, blue-green pigment pyocyanine and HCN (PubMed:11673439). Increases levels of autoinducers 3-oxo-C12-HSL (3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-dodecanamide) and C4-HSL (N-butanoylhomoserine lactone) in early log phase which becomes nearly wild-type (3-oxo-C12-HSL) or 2-fold greater (C4-HSL) by late log phase (PubMed:11673439). Loss of swarming mobility and rhamnolipid production, loss of sRNA RsmZ expression, decreased lipase, increases synthesis of pyocyanine and HCN (PubMed:15126453). Loss of swarming mobility, overproduction of pyocyanine, reduction in lipase biosynthesis (PubMed:16359708).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10E → A: Behaves like wild-type in swarming, pyocyanine production and lipase biosynthesis assays. 1 Publication1
Mutagenesisi44R → A: Does not complement deletion in swarming, pyocyanine production or lipase biosynthesis assays. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001770801 – 61Translational regulator CsrAAdd BLAST61

Proteomic databases

PaxDbiO69078

Expressioni

Inductioni

Low levels of expression in early log phase, increases about 3-fold by stationary phase (at protein level) (PubMed:11673439).1 Publication

Interactioni

Subunit structurei

Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core while the alpha-helices form wings that extend away from the core (PubMed:16104018).UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi208964.PA0905

Structurei

Secondary structure

161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi11 – 14Combined sources4
Turni15 – 17Combined sources3
Beta strandi18 – 26Combined sources9
Beta strandi29 – 37Combined sources9
Beta strandi42 – 44Combined sources3
Helixi45 – 54Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VPZX-ray2.05A/B1-61[»]
ProteinModelPortaliO69078
SMRiO69078
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO69078

Family & Domainsi

Sequence similaritiesi

Belongs to the CsrA/RsmA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105VM8 Bacteria
COG1551 LUCA
HOGENOMiHOG000020767
InParanoidiO69078
KOiK03563
OMAiIHRKEVY
PhylomeDBiO69078

Family and domain databases

Gene3Di3.30.70.1680, 1 hit
HAMAPiMF_00167 CsrA, 1 hit
InterProiView protein in InterPro
IPR003751 CsrA
IPR036107 CsrA_sf
PANTHERiPTHR34984 PTHR34984, 1 hit
PfamiView protein in Pfam
PF02599 CsrA, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009007 CsrA, 1 hit
SUPFAMiSSF117130 SSF117130, 1 hit
TIGRFAMsiTIGR00202 csrA, 1 hit

Sequencei

Sequence statusi: Complete.

O69078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLILTRRVGE TLMVGDDVTV TVLGVKGNQV RIGVNAPKEV AVHREEIYQR
60
IQKEKDQEPN H
Length:61
Mass (Da):6,909
Last modified:August 1, 1998 - v1
Checksum:i3F5EDCD8AD28BB10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061757 Genomic DNA Translation: AAC16242.1
AE004091 Genomic DNA Translation: AAG04294.1
PIRiD83531
RefSeqiNP_249596.1, NC_002516.2
WP_003085981.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG04294; AAG04294; PA0905
GeneIDi34383519
878352
KEGGipae:PA0905
PATRICifig|208964.12.peg.940

Similar proteinsi

Entry informationi

Entry nameiCSRA_PSEAE
AccessioniPrimary (citable) accession number: O69078
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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