O69077 (AK_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartokinase
EC=2.7.2.4

Alternative name(s):
Aspartate kinase

Gene names

Name:	lysC
Ordered Locus Names:	PA0904

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Aspartokinase		PRO_0000066679
Regions
	Domain	265 – 336	72	ACT 1
	Domain	345 – 410	66	ACT 2

Sequences

	Sequence		Length	Mass (Da)
	O69077 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: A06ACAA5E914F337	FASTA	412	44,389
	10 20 30 40 50 60 MALIVQKFGG TSVGTVERIE QVAEKVKKFR EAGDDVVVVV SAMSGETNRL IGLANQIMEQ 70 80 90 100 110 120 PVPRELDVMV STGEQVTIAL LSMALIKRGV PAVSYTGNQV RILTDSAHTK ARILHIDDTH 130 140 150 160 170 180 IRADLKAGRV VVVAGFQGVD GNGNITTLGR GGSDTTGVAL AAALKADECQ IYTDVDGVYT 190 200 210 220 230 240 TDPRVVPQAR RLDKITFEEM LEMASLGSKV LQIRAVEFAG KYNVPLRVLH SFQEGPGTLI 250 260 270 280 290 300 TIDDEEESME QPIISGIAFN RDEAKLTIRG VPDTPGVAFK ILGPISAANV EVDMIVQNVA 310 320 330 340 350 360 HDNTTDFTFT VHRNDYLNAL EILKQTAANI GAREAIGDTN IAKVSIVGVG MRSHAGVASR 370 380 390 400 410 MFEALAKESI NIQMISTSEI KVSVVIEEKY LELAVRALHT AFELDAPARQ GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	Hindle Z., Throup J.P., Francis K.P., Bycroft B.W., Williams P., Stewart G.S.A.B. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 198-412. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04293.1. AF061757 Genomic DNA. Translation: AAC16241.1.
PIR	C83531.
RefSeq	NP_249595.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O69077.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878315.
GenomeReviews	Gene locus PA0904 in contig AE004091_GR.
KEGG	pae:PA0904.
PATRIC	19836094. VBIPseAer58763_0939.
Organism-specific databases
PseudoCAP	PA0904.
Phylogenomic databases
HOGENOM	HBG724395.
OMA	VHVRSSF.
ProtClustDB	PRK06635.
Enzyme and pathway databases
BioCyc	PAER208964:PA0904-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00928.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
PIRSF	PIRSF000726. Asp_kin. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00656. Asp_kin_monofn. 1 hit. TIGR00657. Asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_PSEAE

Accession

Primary (citable) accession number: O69077
Secondary accession number(s): Q9I552

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program