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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-1457-MONOMER
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Synonyms:dutA
Ordered Locus Names:CT1418
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828481 – 152Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST152

Interactioni

Protein-protein interaction databases

STRINGi194439.CT1418

Structurei

3D structure databases

ProteinModelPortaliO68992
SMRiO68992
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 67Substrate bindingUniRule annotation3
Regioni82 – 84Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z1K Bacteria
COG0756 LUCA
HOGENOMiHOG000028966
KOiK01520
OMAiGVILINH
OrthoDBiPOG091H01I6

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00116 dUTPase_bact, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

O68992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVKIVRLN QKAILPVYAT AHAAGMDVSA CLDAPVTVPS SASALIPTGF
60 70 80 90 100
AIELPEGYEA QLRPRSGLAL RHCISLPNSP ATIDADYRGE VGVILINHGR
110 120 130 140 150
EPFTVSHGDR IAQMVVAKVD HVVFEEVESL SETARGEGGF GHTGVQAKAE

CL
Length:152
Mass (Da):16,098
Last modified:August 30, 2002 - v2
Checksum:i5A95DA98FD41BEE1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61 – 62QL → HV in AAC14879 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060080 Genomic DNA Translation: AAC14879.1
AY005138 Genomic DNA Translation: AAG12427.1
AE006470 Genomic DNA Translation: AAM72646.1
RefSeqiNP_662304.1, NC_002932.3
WP_010933085.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM72646; AAM72646; CT1418
GeneIDi1006834
KEGGicte:CT1418
PATRICifig|194439.7.peg.1286

Similar proteinsi

Entry informationi

Entry nameiDUT_CHLTE
AccessioniPrimary (citable) accession number: O68992
Secondary accession number(s): Q93ST9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 30, 2002
Last modified: March 28, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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