O68992 (DUT_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Synonyms:	dutA
Ordered Locus Names:	CT1418

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000182848
Regions
	Region	65 – 67	3	Substrate binding By similarity
	Region	82 – 84	3	Substrate binding By similarity
Sites
	Binding site	78	1	Substrate By similarity
Experimental info
	Sequence conflict	61 – 62	2	QL → HV in AAC14879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O68992 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 5A95DA98FD41BEE1	FASTA	152	16,098
	10 20 30 40 50 60 MIKVKIVRLN QKAILPVYAT AHAAGMDVSA CLDAPVTVPS SASALIPTGF AIELPEGYEA 70 80 90 100 110 120 QLRPRSGLAL RHCISLPNSP ATIDADYRGE VGVILINHGR EPFTVSHGDR IAQMVVAKVD 130 140 150 HVVFEEVESL SETARGEGGF GHTGVQAKAE CL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Bryant D.A., Jakobs C., Stirewalt V.L. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular evidence for the early evolution of photosynthesis." Xiong J., Fischer W.M., Inoue K., Nakahara M., Bauer C.E. Science 289:1724-1730(2000) [PubMed: 10976061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF060080 Genomic DNA. Translation: AAC14879.1. AY005138 Genomic DNA. Translation: AAG12427.1. AE006470 Genomic DNA. Translation: AAM72646.1.
RefSeq	NP_662304.1. NC_002932.3.
3D structure databases
ProteinModelPortal	O68992.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006834.
GenomeReviews	Gene locus CT1418 in contig AE006470_GR.
KEGG	cte:CT1418.
NMPDR	fig|194439.1.peg.1398.
PATRIC	21400763. VBIChlTep116050_1286.
TIGR	CT1418.
Phylogenomic databases
HOGENOM	HBG436079.
OMA	LDLRACI.
ProtClustDB	CLSK637750.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1418-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_CHLTE

Accession

Primary (citable) accession number: O68992
Secondary accession number(s): Q93ST9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program