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Protein

Inositol 2-dehydrogenase

Gene

idhA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the oxidation of myo-inositol (MI) to 2-keto-myo-inositol (2KMI or 2-inosose).1 Publication

Catalytic activityi

Myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH.

Pathwayi: myo-inositol degradation into acetyl-CoA

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from myo-inositol.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Inositol 2-dehydrogenase (idhA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. no protein annotated in this organism
This subpathway is part of the pathway myo-inositol degradation into acetyl-CoA, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from myo-inositol, the pathway myo-inositol degradation into acetyl-CoA and in Polyol metabolism.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-4627-MONOMER.
BRENDAi1.1.1.18. 5347.
UniPathwayiUPA00076; UER00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol 2-dehydrogenase (EC:1.1.1.18)
Alternative name(s):
Myo-inositol 2-dehydrogenase
Short name:
MI-dehydrogenase
Gene namesi
Name:idhA
Synonyms:iolG
Ordered Locus Names:RB1194
ORF Names:SMb20899
Encoded oniPlasmid pSymB (megaplasmid 2)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymB

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Inositol 2-dehydrogenasePRO_0000091775Add
BLAST

Expressioni

Inductioni

By myo-inositol.1 Publication

Structurei

Secondary structure

1
330
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2312Combined sources
Beta strandi27 – 337Combined sources
Helixi37 – 4610Combined sources
Helixi54 – 596Combined sources
Beta strandi65 – 684Combined sources
Helixi72 – 743Combined sources
Helixi75 – 8410Combined sources
Beta strandi88 – 914Combined sources
Helixi99 – 11113Combined sources
Beta strandi116 – 1183Combined sources
Helixi121 – 1244Combined sources
Helixi126 – 13611Combined sources
Turni137 – 1404Combined sources
Beta strandi142 – 15110Combined sources
Helixi158 – 1625Combined sources
Turni163 – 1653Combined sources
Helixi167 – 1704Combined sources
Helixi172 – 18312Combined sources
Beta strandi187 – 1959Combined sources
Helixi200 – 2045Combined sources
Beta strandi209 – 2179Combined sources
Beta strandi222 – 2298Combined sources
Beta strandi236 – 24510Combined sources
Beta strandi247 – 2504Combined sources
Beta strandi258 – 2625Combined sources
Beta strandi265 – 2684Combined sources
Helixi275 – 2784Combined sources
Helixi280 – 29516Combined sources
Helixi304 – 32320Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HKTX-ray2.00A/B/C/D1-330[»]
ProteinModelPortaliO68965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Gfo/Idh/MocA family.Curated

Phylogenomic databases

KOiK00010.
OMAiYDQRVEI.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR030827. Myo_inos_iolG.
IPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR004104. OxRdtase_C.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
PF02894. GFO_IDH_MocA_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR04380. myo_inos_iolG. 1 hit.

Sequencei

Sequence statusi: Complete.

O68965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVRFGLLGA GRIGKVHAKA VSGNADARLV AVADAFPAAA EAIAGAYGCE
60 70 80 90 100
VRTIDAIEAA ADIDAVVICT PTDTHADLIE RFARAGKAIF CEKPIDLDAE
110 120 130 140 150
RVRACLKVVS DTKAKLMVGF NRRFDPHFMA VRKAIDDGRI GEVEMVTITS
160 170 180 190 200
RDPSAPPVDY IKRSGGIFRD MTIHDFDMAR FLLGEEPVSV TATAAVLIDK
210 220 230 240 250
AIGDAGDYDS VSVILQTASG KQAIISNSRR ATYGYDQRIE VHGSKGAVAA
260 270 280 290 300
ENQRPVSIEI ATGDGYTRPP LHDFFMTRYT EAYANEIESF IAAIEKGAEI
310 320 330
APSGNDGLAA LALADAAVRS VAEKRQISIA
Length:330
Mass (Da):35,147
Last modified:November 2, 2001 - v2
Checksum:i06F5FBBAC7484A58
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261S → A in AAC70005 (PubMed:9802033).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059313 Genomic DNA. Translation: AAC70005.1.
AL591985 Genomic DNA. Translation: CAC49594.1.
PIRiB95991.
RefSeqiNP_437734.1. NC_003078.1.
WP_010976021.1. NC_003078.1.

Genome annotation databases

EnsemblBacteriaiCAC49594; CAC49594; SM_b20899.
GeneIDi1237525.
KEGGisme:SM_b20899.
PATRICi23638800. VBISinMel96828_6121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059313 Genomic DNA. Translation: AAC70005.1.
AL591985 Genomic DNA. Translation: CAC49594.1.
PIRiB95991.
RefSeqiNP_437734.1. NC_003078.1.
WP_010976021.1. NC_003078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HKTX-ray2.00A/B/C/D1-330[»]
ProteinModelPortaliO68965.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC49594; CAC49594; SM_b20899.
GeneIDi1237525.
KEGGisme:SM_b20899.
PATRICi23638800. VBISinMel96828_6121.

Phylogenomic databases

KOiK00010.
OMAiYDQRVEI.

Enzyme and pathway databases

UniPathwayiUPA00076; UER00143.
BioCyciSMEL266834:GJF6-4627-MONOMER.
BRENDAi1.1.1.18. 5347.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR030827. Myo_inos_iolG.
IPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR004104. OxRdtase_C.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
PF02894. GFO_IDH_MocA_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR04380. myo_inos_iolG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMI2D_RHIME
AccessioniPrimary (citable) accession number: O68965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.