Reviewed, UniProtKB/Swiss-Prot O68965 (MI2D_RHIME)

Names and origin

Protein names

Recommended name:
    Inositol 2-dehydrogenase
    EC=1.1.1.18
Alternative name(s):
    Myo-inositol 2-dehydrogenase
      Short name=MI-dehydrogenase

Gene names

Name:	idhA
Synonyms:	iolG
Ordered Locus Names:	RB1194
ORF Names:	SMb20899

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the oxidation of myo-inositol (MI) to 2-keto-myo-inositol (2KMI or 2-inosose). Ref.1
Catalytic activity	Myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 1/7.
Induction	By myo-inositol. Ref.1
Sequence similarities	Belongs to the gfo/idh/mocA family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro inositol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Inositol 2-dehydrogenase		PRO_0000091775
Experimental info
	Sequence conflict	226	1	S → A in AAC70005. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O68965-1 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 06F5FBBAC7484A58	FASTA	330	35,147
	10 20 30 40 50 60 MTVRFGLLGA GRIGKVHAKA VSGNADARLV AVADAFPAAA EAIAGAYGCE VRTIDAIEAA 70 80 90 100 110 120 ADIDAVVICT PTDTHADLIE RFARAGKAIF CEKPIDLDAE RVRACLKVVS DTKAKLMVGF 130 140 150 160 170 180 NRRFDPHFMA VRKAIDDGRI GEVEMVTITS RDPSAPPVDY IKRSGGIFRD MTIHDFDMAR 190 200 210 220 230 240 FLLGEEPVSV TATAAVLIDK AIGDAGDYDS VSVILQTASG KQAIISNSRR ATYGYDQRIE 250 260 270 280 290 300 VHGSKGAVAA ENQRPVSIEI ATGDGYTRPP LHDFFMTRYT EAYANEIESF IAAIEKGAEI 310 320 330 APSGNDGLAA LALADAAVRS VAEKRQISIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A functional myo-inositol catabolism pathway is essential for rhizopine utilization by Sinorhizobium meliloti." Galbraith M.P., Feng S.F., Borneman J., Triplett E.W., de Bruijn F.J., Rossbach S. Microbiology 144:2915-2924(1998) [PubMed: 9802033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION AS MI-DEHYDROGENASE. Strain: 1021.
[2]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF059313 Genomic DNA. Translation: AAC70005.1. AL591985 Genomic DNA. Translation: CAC49594.1.
PIR	B95991.
RefSeq	NP_437734.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1237525.
GenomeReviews	Gene locus RB1194 in contig AL591985_GR.
KEGG	sme:SM_b20899.
NMPDR	fig|266834.1.peg.5829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O68965.
OMA	O68965. LERYMQS.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB20899-MON.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000683. Oxidoreductase_N. IPR004104. OxRdtase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01408. GFO_IDH_MocA. 1 hit. PF02894. GFO_IDH_MocA_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MI2D_RHIME

Accession

Primary (citable) accession number: O68965

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 2, 2001
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)