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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei623 – 6231For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1568-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:MSMEG_1568, MSMEI_1531
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 628627Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135356Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_1568.

Structurei

3D structure databases

ProteinModelPortaliO68956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 229228Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini301 – 440140SIS 1UniRule annotationAdd
BLAST
Domaini473 – 618146SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O68956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGYVGH RPARDIVVDA LRRMEYRGYD SAGIALIDGN GGLTVRRRAG
60 70 80 90 100
RLANLEATLA ETDSNDGDGL GGSTGLGHTR WATHGRPTDR NAHPHRDAAG
110 120 130 140 150
KIAVVHNGII ENFAPLRAEL EAAGVEFASD TDTEVAVHLV ARQYTQGDTA
160 170 180 190 200
GDFPASVLAV LQRLEGHFTL VFASADDPGT IVAARRSTPL VLGIGDGEMF
210 220 230 240 250
VGSDVAAFIE HTRDAVELGQ DQAVVLTADG YRITDFAGND HLEAGRDFRE
260 270 280 290 300
FHIDWDLNAA EKGGYDYFML KEIAEQPSAV ADTLLGHFDK NRIVLDEQRL
310 320 330 340 350
SDQELREIDK VFIVACGTAY HSGLLAKYAI EHWTRLPVEV ELASEFRYRD
360 370 380 390 400
PVLDRSTLVI AISQSGETAD TLEAVRHAKT QKAKVLAICN TNGSQIPREA
410 420 430 440 450
DAVLYTRAGP EIGVAATKTF LAQIAANYLV GLALAQARGT KYPDEVAREY
460 470 480 490 500
RELEAMPDLI KRVLAGMDSV AALAERFAPS STVLFLGRHV GYPVALEGAL
510 520 530 540 550
KLKELAYMHA EGFAAGELKH GPIALIDENL PVIVVMPSPK NAAMLHAKLL
560 570 580 590 600
SNIREIQARG AVTVVIAEED DDTVRPYADH LIEIPSVSTL FQPLLSTIPL
610 620
QVFAAGVARA RGYDVDKPRN LAKSVTVE
Length:628
Mass (Da):67,903
Last modified:January 23, 2007 - v3
Checksum:i0A9E6034BE0577E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058788 Genomic DNA. Translation: AAC14295.1.
CP000480 Genomic DNA. Translation: ABK71697.1.
CP001663 Genomic DNA. Translation: AFP38004.1.
RefSeqiYP_006566299.1. NC_018289.1.
YP_885947.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71697; ABK71697; MSMEG_1568.
AFP38004; AFP38004; MSMEI_1531.
GeneIDi4536717.
KEGGimsm:MSMEG_1568.
PATRICi18075597. VBIMycSme59918_1555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058788 Genomic DNA. Translation: AAC14295.1.
CP000480 Genomic DNA. Translation: ABK71697.1.
CP001663 Genomic DNA. Translation: AFP38004.1.
RefSeqiYP_006566299.1. NC_018289.1.
YP_885947.1. NC_008596.1.

3D structure databases

ProteinModelPortaliO68956.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1568.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71697; ABK71697; MSMEG_1568.
AFP38004; AFP38004; MSMEI_1531.
GeneIDi4536717.
KEGGimsm:MSMEG_1568.
PATRICi18075597. VBIMycSme59918_1555.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1568-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
    Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiGLMS_MYCS2
AccessioniPrimary (citable) accession number: O68956
Secondary accession number(s): A0QSQ9, I7G5X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.