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O68956

- GLMS_MYCS2

UniProt

O68956 - GLMS_MYCS2

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
    Active sitei623 – 6231For Fru-6P isomerization activityUniRule annotation

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-1568-MONOMER.

    Protein family/group databases

    MEROPSiC44.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
    Alternative name(s):
    D-fructose-6-phosphate amidotransferaseUniRule annotation
    GFATUniRule annotation
    Glucosamine-6-phosphate synthaseUniRule annotation
    Hexosephosphate aminotransferaseUniRule annotation
    L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
    Gene namesi
    Name:glmSUniRule annotation
    Ordered Locus Names:MSMEG_1568, MSMEI_1531
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 628627Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135356Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246196.MSMEG_1568.

    Structurei

    3D structure databases

    ProteinModelPortaliO68956.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 229228Glutamine amidotransferase type-2UniRule annotationAdd
    BLAST
    Domaini301 – 440140SIS 1UniRule annotationAdd
    BLAST
    Domaini473 – 618146SIS 2UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
    Contains 2 SIS domains.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    HOGENOMiHOG000258896.
    KOiK00820.
    OMAiFYTHAGP.
    OrthoDBiEOG6KT2Q1.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00164. GlmS.
    InterProiIPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O68956-1 [UniParc]FASTAAdd to Basket

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    MCGIVGYVGH RPARDIVVDA LRRMEYRGYD SAGIALIDGN GGLTVRRRAG    50
    RLANLEATLA ETDSNDGDGL GGSTGLGHTR WATHGRPTDR NAHPHRDAAG 100
    KIAVVHNGII ENFAPLRAEL EAAGVEFASD TDTEVAVHLV ARQYTQGDTA 150
    GDFPASVLAV LQRLEGHFTL VFASADDPGT IVAARRSTPL VLGIGDGEMF 200
    VGSDVAAFIE HTRDAVELGQ DQAVVLTADG YRITDFAGND HLEAGRDFRE 250
    FHIDWDLNAA EKGGYDYFML KEIAEQPSAV ADTLLGHFDK NRIVLDEQRL 300
    SDQELREIDK VFIVACGTAY HSGLLAKYAI EHWTRLPVEV ELASEFRYRD 350
    PVLDRSTLVI AISQSGETAD TLEAVRHAKT QKAKVLAICN TNGSQIPREA 400
    DAVLYTRAGP EIGVAATKTF LAQIAANYLV GLALAQARGT KYPDEVAREY 450
    RELEAMPDLI KRVLAGMDSV AALAERFAPS STVLFLGRHV GYPVALEGAL 500
    KLKELAYMHA EGFAAGELKH GPIALIDENL PVIVVMPSPK NAAMLHAKLL 550
    SNIREIQARG AVTVVIAEED DDTVRPYADH LIEIPSVSTL FQPLLSTIPL 600
    QVFAAGVARA RGYDVDKPRN LAKSVTVE 628
    Length:628
    Mass (Da):67,903
    Last modified:January 23, 2007 - v3
    Checksum:i0A9E6034BE0577E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF058788 Genomic DNA. Translation: AAC14295.1.
    CP000480 Genomic DNA. Translation: ABK71697.1.
    CP001663 Genomic DNA. Translation: AFP38004.1.
    RefSeqiYP_006566299.1. NC_018289.1.
    YP_885947.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK71697; ABK71697; MSMEG_1568.
    AFP38004; AFP38004; MSMEI_1531.
    GeneIDi4536717.
    KEGGimsg:MSMEI_1531.
    msm:MSMEG_1568.
    PATRICi18075597. VBIMycSme59918_1555.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF058788 Genomic DNA. Translation: AAC14295.1 .
    CP000480 Genomic DNA. Translation: ABK71697.1 .
    CP001663 Genomic DNA. Translation: AFP38004.1 .
    RefSeqi YP_006566299.1. NC_018289.1.
    YP_885947.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali O68956.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_1568.

    Protein family/group databases

    MEROPSi C44.971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK71697 ; ABK71697 ; MSMEG_1568 .
    AFP38004 ; AFP38004 ; MSMEI_1531 .
    GeneIDi 4536717.
    KEGGi msg:MSMEI_1531.
    msm:MSMEG_1568.
    PATRICi 18075597. VBIMycSme59918_1555.

    Phylogenomic databases

    eggNOGi COG0449.
    HOGENOMi HOG000258896.
    KOi K00820.
    OMAi FYTHAGP.
    OrthoDBi EOG6KT2Q1.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-1568-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00164. GlmS.
    InterProi IPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
      Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiGLMS_MYCS2
    AccessioniPrimary (citable) accession number: O68956
    Secondary accession number(s): A0QSQ9, I7G5X8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3