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O68956

- GLMS_MYCS2

UniProt

O68956 - GLMS_MYCS2

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
glmS, MSMEG_1568, MSMEI_1531
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activity By similarity
Active sitei623 – 6231For Fru-6P isomerization activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1568-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:MSMEG_1568, MSMEI_1531
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 628627Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation
PRO_0000135356Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_1568.

Structurei

3D structure databases

ProteinModelPortaliO68956.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 229228Glutamine amidotransferase type-2
Add
BLAST
Domaini301 – 440140SIS 1
Add
BLAST
Domaini473 – 618146SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiFYTHAGP.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O68956-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIVGYVGH RPARDIVVDA LRRMEYRGYD SAGIALIDGN GGLTVRRRAG    50
RLANLEATLA ETDSNDGDGL GGSTGLGHTR WATHGRPTDR NAHPHRDAAG 100
KIAVVHNGII ENFAPLRAEL EAAGVEFASD TDTEVAVHLV ARQYTQGDTA 150
GDFPASVLAV LQRLEGHFTL VFASADDPGT IVAARRSTPL VLGIGDGEMF 200
VGSDVAAFIE HTRDAVELGQ DQAVVLTADG YRITDFAGND HLEAGRDFRE 250
FHIDWDLNAA EKGGYDYFML KEIAEQPSAV ADTLLGHFDK NRIVLDEQRL 300
SDQELREIDK VFIVACGTAY HSGLLAKYAI EHWTRLPVEV ELASEFRYRD 350
PVLDRSTLVI AISQSGETAD TLEAVRHAKT QKAKVLAICN TNGSQIPREA 400
DAVLYTRAGP EIGVAATKTF LAQIAANYLV GLALAQARGT KYPDEVAREY 450
RELEAMPDLI KRVLAGMDSV AALAERFAPS STVLFLGRHV GYPVALEGAL 500
KLKELAYMHA EGFAAGELKH GPIALIDENL PVIVVMPSPK NAAMLHAKLL 550
SNIREIQARG AVTVVIAEED DDTVRPYADH LIEIPSVSTL FQPLLSTIPL 600
QVFAAGVARA RGYDVDKPRN LAKSVTVE 628
Length:628
Mass (Da):67,903
Last modified:January 23, 2007 - v3
Checksum:i0A9E6034BE0577E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF058788 Genomic DNA. Translation: AAC14295.1.
CP000480 Genomic DNA. Translation: ABK71697.1.
CP001663 Genomic DNA. Translation: AFP38004.1.
RefSeqiYP_006566299.1. NC_018289.1.
YP_885947.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71697; ABK71697; MSMEG_1568.
AFP38004; AFP38004; MSMEI_1531.
GeneIDi4536717.
KEGGimsg:MSMEI_1531.
msm:MSMEG_1568.
PATRICi18075597. VBIMycSme59918_1555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF058788 Genomic DNA. Translation: AAC14295.1 .
CP000480 Genomic DNA. Translation: ABK71697.1 .
CP001663 Genomic DNA. Translation: AFP38004.1 .
RefSeqi YP_006566299.1. NC_018289.1.
YP_885947.1. NC_008596.1.

3D structure databases

ProteinModelPortali O68956.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_1568.

Protein family/group databases

MEROPSi C44.971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK71697 ; ABK71697 ; MSMEG_1568 .
AFP38004 ; AFP38004 ; MSMEI_1531 .
GeneIDi 4536717.
KEGGi msg:MSMEI_1531.
msm:MSMEG_1568.
PATRICi 18075597. VBIMycSme59918_1555.

Phylogenomic databases

eggNOGi COG0449.
HOGENOMi HOG000258896.
KOi K00820.
OMAi FYTHAGP.
OrthoDBi EOG6KT2Q1.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-1568-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
    Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiGLMS_MYCS2
AccessioniPrimary (citable) accession number: O68956
Secondary accession number(s): A0QSQ9, I7G5X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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