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Protein

Serine protease pet autotransporter

Gene

pet

Organism
Escherichia coli O44:H18 (strain 042 / EAEC)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell.4 Publications

Enzyme regulationi

Inhibition of cytotoxic activity by phenylmethylsulfonyl fluoride.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Charge relay systemPROSITE-ProRule annotation1
Active sitei153Charge relay systemPROSITE-ProRule annotation1
Active sitei260Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciECOL216592:GCV7-4960-MONOMER.

Protein family/group databases

MEROPSiS06.004.
TCDBi1.B.12.4.4. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease pet autotransporter (EC:3.4.21.-)
Cleaved into the following 2 chains:
Alternative name(s):
Plasmid-encoded toxin pet
Gene namesi
Name:pet
Ordered Locus Names:EC042_pAA035
Encoded oniPlasmid pAA20 Publication
OrganismiEscherichia coli O44:H18 (strain 042 / EAEC)
Taxonomic identifieri216592 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001407 Componenti: Plasmid pAA

Subcellular locationi

Serine protease pet translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi260S → I: Loss of protease activity, but does not affect secretion. 1 Publication1
Mutagenesisi1018 – 1019NN → GI: Abolishes secretion. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 521 PublicationAdd BLAST52
ChainiPRO_000038760453 – 1295Serine protease pet autotransporterAdd BLAST1243
ChainiPRO_000002697053 – 1018Serine protease petAdd BLAST966
ChainiPRO_00000269711019 – 1295Serine protease pet translocatorAdd BLAST277

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1018 – 1019Cleavage2

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiO68900.

Structurei

Secondary structure

11295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni57 – 59Combined sources3
Helixi62 – 69Combined sources8
Beta strandi112 – 115Combined sources4
Beta strandi118 – 122Combined sources5
Helixi123 – 125Combined sources3
Beta strandi128 – 137Combined sources10
Beta strandi140 – 149Combined sources10
Beta strandi154 – 161Combined sources8
Helixi179 – 186Combined sources8
Beta strandi198 – 203Combined sources6
Beta strandi224 – 237Combined sources14
Beta strandi240 – 247Combined sources8
Beta strandi249 – 251Combined sources3
Helixi257 – 259Combined sources3
Beta strandi263 – 268Combined sources6
Turni269 – 272Combined sources4
Beta strandi273 – 287Combined sources15
Beta strandi291 – 296Combined sources6
Helixi299 – 307Combined sources9
Beta strandi310 – 314Combined sources5
Beta strandi318 – 323Combined sources6
Beta strandi346 – 351Combined sources6
Beta strandi353 – 359Combined sources7
Beta strandi368 – 370Combined sources3
Beta strandi375 – 380Combined sources6
Helixi381 – 383Combined sources3
Beta strandi387 – 391Combined sources5
Beta strandi393 – 395Combined sources3
Beta strandi400 – 407Combined sources8
Beta strandi413 – 423Combined sources11
Beta strandi431 – 433Combined sources3
Beta strandi435 – 440Combined sources6
Beta strandi449 – 451Combined sources3
Beta strandi456 – 460Combined sources5
Turni469 – 473Combined sources5
Beta strandi474 – 476Combined sources3
Beta strandi481 – 484Combined sources4
Beta strandi490 – 493Combined sources4
Beta strandi503 – 505Combined sources3
Beta strandi509 – 511Combined sources3
Beta strandi513 – 517Combined sources5
Beta strandi523 – 525Combined sources3
Beta strandi527 – 529Combined sources3
Beta strandi531 – 538Combined sources8
Beta strandi548 – 550Combined sources3
Beta strandi554 – 564Combined sources11
Beta strandi566 – 568Combined sources3
Beta strandi580 – 582Combined sources3
Turni589 – 591Combined sources3
Helixi595 – 602Combined sources8
Helixi604 – 610Combined sources7
Helixi613 – 615Combined sources3
Beta strandi616 – 618Combined sources3
Beta strandi631 – 647Combined sources17
Beta strandi651 – 661Combined sources11
Beta strandi663 – 666Combined sources4
Beta strandi669 – 674Combined sources6
Turni675 – 678Combined sources4
Turni683 – 686Combined sources4
Beta strandi692 – 696Combined sources5
Beta strandi701 – 705Combined sources5
Beta strandi707 – 716Combined sources10
Beta strandi720 – 724Combined sources5
Beta strandi728 – 732Combined sources5
Beta strandi734 – 737Combined sources4
Beta strandi742 – 745Combined sources4
Beta strandi749 – 753Combined sources5
Beta strandi755 – 764Combined sources10
Beta strandi769 – 772Combined sources4
Beta strandi777 – 781Combined sources5
Beta strandi785 – 787Combined sources3
Beta strandi792 – 803Combined sources12
Beta strandi805 – 807Combined sources3
Beta strandi818 – 820Combined sources3
Turni833 – 835Combined sources3
Beta strandi836 – 844Combined sources9
Beta strandi846 – 848Combined sources3
Beta strandi874 – 876Combined sources3
Beta strandi893 – 895Combined sources3
Beta strandi967 – 969Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OM9X-ray2.30A53-1018[»]
ProteinModelPortaliO68900.
SMRiO68900.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 304Peptidase S6PROSITE-ProRule annotationAdd BLAST251
Domaini1029 – 1295AutotransporterPROSITE-ProRule annotationAdd BLAST267

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage.

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

HOGENOMiHOG000117212.
KOiK12684.
OMAiLVMQGHA.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O68900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIYSIKYS AATGGLIAVS ELAKKVICKT NRKISAALLS LAVISYTNII
60 70 80 90 100
YAANMDISKA WARDYLDLAQ NKGVFQPGST HVKIKLKDGT DFSFPALPVP
110 120 130 140 150
DFSSATANGA ATSIGGAYAV TVAHNAKNKS SANYQTYGST QYTQINRMTT
160 170 180 190 200
GNDFSIQRLN KYVVETRGAD TSFNYNENNQ NIIDRYGVDV GNGKKEIIGF
210 220 230 240 250
RVGSGNTTFS GIKTSQTYQA DLLSASLFHI TNLRANTVGG NKVEYENDSY
260 270 280 290 300
FTNLTTNGDS GSGVYVFDNK EDKWVLLGTT HGIIGNGKTQ KTYVTPFDSK
310 320 330 340 350
TTNELKQLFI QNVNIDNNTA TIGGGKITIG NTTQDIEKNK NNQNKDLVFS
360 370 380 390 400
GGGKISLKEN LDLGYGGFIF DENKKYTVSA EGNNNVTFKG AGIDIGKGST
410 420 430 440 450
VDWNIKYASN DALHKIGEGS LNVIQAQNTN LKTGNGTVIL GAQKTFNNIY
460 470 480 490 500
VAGGPGTVQL NAENALGEGD YAGIFFTENG GKLDLNGHNQ TFKKIAATDS
510 520 530 540 550
GTTITNSNTT KESVLSVNNQ NNYIYHGNVD GNVRLEHHLD TKQDNARLIL
560 570 580 590 600
DGDIQANSIS IKNAPLVMQG HATDHAIFRT TKTNNCPEFL CGVDWVTRIK
610 620 630 640 650
NAENSVNQKN KTTYKSNNQV SDLSQPDWET RKFRFDNLNI EDSSLSIARN
660 670 680 690 700
ADVEGNIQAK NSVINIGDKT AYIDLYSGKN ITGAGFTFRQ DIKSGDSIGE
710 720 730 740 750
SKFTGGIMAT DGSISIGDKA IVTLNTVSSL DRTALTIHKG ANVTASSSLF
760 770 780 790 800
TTSNIKSGGD LTLTGATEST GEITPSMFYA AGGYELTEDG ANFTAKNQAS
810 820 830 840 850
VTGDIKSEKA AKLSFGSADK DNSATRYSQF ALAMLDGFDT SYQGSIKAAQ
860 870 880 890 900
SSLAMNNALW KVTGNSELKK LNSTGSMVLF NGGKNIFNTL TVDELTTSNS
910 920 930 940 950
AFVMRTNTQQ ADQLIVKNKL EGANNLLLVD FIEKKGNDKN GLNIDLVKAP
960 970 980 990 1000
ENTSKDVFKT ETQTIGFSDV TPEIKQQEKD GKSVWTLTGY KTVANADAAK
1010 1020 1030 1040 1050
KATSLMSGGY KAFLAEVNNL NKRMGDLRDI NGEAGAWARI MSGTGSAGGG
1060 1070 1080 1090 1100
FSDNYTHVQV GADNKHELDG LDLFTGVTMT YTDSHAGSDA FSGETKSVGA
1110 1120 1130 1140 1150
GLYASAMFES GAYIDLIGKY VHHDNEYTAT FAGLGTRDYS SHSWYAGAEV
1160 1170 1180 1190 1200
GYRYHVTDSA WIEPQAELVY GAVSGKQFSW KDQGMNLTMK DKDFNPLIGR
1210 1220 1230 1240 1250
TGVDVGKSFS GKDWKVTARA GLGYQFDLFA NGETVLRDAS GEKRIKGEKD
1260 1270 1280 1290
GRMLMNVGLN AEIRDNVRFG LEFEKSAFGK YNVDNAINAN FRYSF
Length:1,295
Mass (Da):139,769
Last modified:August 1, 1998 - v1
Checksum:i9C6AEF7F345AD429
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056581 Genomic DNA. Translation: AAC26634.1.
FN554767 Genomic DNA. Translation: CBG27789.1.
RefSeqiWP_014639426.1. NC_017627.1.

Genome annotation databases

KEGGielo:EC042_pAA035.
PATRICi36691751. VBIEscCol52250_5129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056581 Genomic DNA. Translation: AAC26634.1.
FN554767 Genomic DNA. Translation: CBG27789.1.
RefSeqiWP_014639426.1. NC_017627.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OM9X-ray2.30A53-1018[»]
ProteinModelPortaliO68900.
SMRiO68900.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS06.004.
TCDBi1.B.12.4.4. the autotransporter-1 (at-1) family.

Proteomic databases

PRIDEiO68900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGielo:EC042_pAA035.
PATRICi36691751. VBIEscCol52250_5129.

Phylogenomic databases

HOGENOMiHOG000117212.
KOiK12684.
OMAiLVMQGHA.

Enzyme and pathway databases

BioCyciECOL216592:GCV7-4960-MONOMER.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPET_ECO44
AccessioniPrimary (citable) accession number: O68900
Secondary accession number(s): D3H579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.