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Protein

Aquaporin Z

Gene

aqpZ

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 201Involved in tetramerization or stability of the tetramer
Sitei43 – 431Selectivity filterUniRule annotation
Sitei174 – 1741Selectivity filterUniRule annotation
Sitei183 – 1831Selectivity filterUniRule annotation
Sitei189 – 1891Selectivity filterUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin ZUniRule annotation
Gene namesi
Name:aqpZUniRule annotation
Synonyms:bniP
Ordered Locus Names:SF0832, S0873
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalUniRule annotationAdd
BLAST
Topological domaini30 – 334PeriplasmicSequence analysis
Transmembranei34 – 5421HelicalUniRule annotationAdd
BLAST
Topological domaini55 – 8127CytoplasmicSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalUniRule annotationAdd
BLAST
Topological domaini103 – 13028PeriplasmicSequence analysisAdd
BLAST
Transmembranei131 – 15121HelicalUniRule annotationAdd
BLAST
Topological domaini152 – 1554CytoplasmicSequence analysis
Transmembranei156 – 17621HelicalUniRule annotationAdd
BLAST
Topological domaini177 – 20125PeriplasmicSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalUniRule annotationAdd
BLAST
Topological domaini223 – 2319CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Aquaporin ZPRO_0000064000Add
BLAST

Proteomic databases

PaxDbiO68874.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF0832.

Structurei

3D structure databases

ProteinModelPortaliO68874.
SMRiO68874. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 653NPA 1
Motifi186 – 1883NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]UniRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8C. Bacteria.
COG0580. LUCA.
HOGENOMiHOG000288286.
KOiK06188.
OMAiMPIIGGI.
OrthoDBiEOG6GFGN7.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
HAMAPiMF_01146. Aquaporin_Z.
InterProiIPR023271. Aquaporin-like.
IPR023743. Aquaporin_Z.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRKLAAECF GTFWLVFGGC GSAVLAAGFP ELGIGFAGVA LAFGLTVLTM
60 70 80 90 100
AFAVGHISGG HFNPAVTIGL WAGGRFPAKE VVGYVIAQVV GGIVAAALLY
110 120 130 140 150
LIASGKTGFD AAASGFASNG YGEHSPGGYS MLSALVVELV LSAGFLLVIH
160 170 180 190 200
GATDKFAPAG FAPIAIGLAL TLIHLISIPV TNTSVNPARS TAVAIFQGGW
210 220 230
ALEQLWFFWV VPIVGGIIGG LIYRTLLEKR D
Length:231
Mass (Da):23,703
Last modified:April 13, 2004 - v2
Checksum:i3BDE1A932D45CF14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431F → I in AAC12651 (Ref. 1) Curated
Sequence conflicti99 – 991L → V in AAC12651 (Ref. 1) Curated
Sequence conflicti176 – 1761I → M in AAC12651 (Ref. 1) Curated
Sequence conflicti227 – 2271L → V in AAC12651 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055905 Genomic DNA. Translation: AAC12651.1.
AE005674 Genomic DNA. Translation: AAN42465.2.
AE014073 Genomic DNA. Translation: AAP16338.1.
RefSeqiNP_706758.2. NC_004337.2.
WP_001298299.1. NZ_LM651928.1.

Genome annotation databases

EnsemblBacteriaiAAN42465; AAN42465; SF0832.
AAP16338; AAP16338; S0873.
GeneIDi1023778.
KEGGisfl:SF0832.
sft:NCTC1_00832.
sfx:S0873.
PATRICi18702865. VBIShiFle31049_0942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055905 Genomic DNA. Translation: AAC12651.1.
AE005674 Genomic DNA. Translation: AAN42465.2.
AE014073 Genomic DNA. Translation: AAP16338.1.
RefSeqiNP_706758.2. NC_004337.2.
WP_001298299.1. NZ_LM651928.1.

3D structure databases

ProteinModelPortaliO68874.
SMRiO68874. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF0832.

Proteomic databases

PaxDbiO68874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN42465; AAN42465; SF0832.
AAP16338; AAP16338; S0873.
GeneIDi1023778.
KEGGisfl:SF0832.
sft:NCTC1_00832.
sfx:S0873.
PATRICi18702865. VBIShiFle31049_0942.

Phylogenomic databases

eggNOGiENOG4105D8C. Bacteria.
COG0580. LUCA.
HOGENOMiHOG000288286.
KOiK06188.
OMAiMPIIGGI.
OrthoDBiEOG6GFGN7.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
HAMAPiMF_01146. Aquaporin_Z.
InterProiIPR023271. Aquaporin-like.
IPR023743. Aquaporin_Z.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the S. flexneri aquaporin-Z coding region."
    Calamita G., Ludovico A., DeVito D., Rizzo G., Svelto M.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiAQPZ_SHIFL
AccessioniPrimary (citable) accession number: O68874
Secondary accession number(s): P48838, P75827, Q47159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 6, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.