Reviewed, UniProtKB/Swiss-Prot O68852 (NUOA1_RHIME)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 1
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 1
    NDH-1 subunit A 1
    NUO1 1

Gene names

Name:	nuoA1
Synonyms:	nuoA
Ordered Locus Names:	R01264
ORF Names:	SMc01912

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 121	121	NADH-quinone oxidoreductase subunit A 1 HAMAP MF_01394		PRO_0000117872
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	65 – 85	21	Potential
	Transmembrane	90 – 110	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O68852-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D3E255E405310163	FASTA	121	13,804
	10 20 30 40 50 60 MTELLGSYVP IAIFIGIALV IGLALLVAPF AVAFKAPDSE KLSAYECGFN AFDDARMKFD 70 80 90 100 110 120 VRFYLVSILF IIFDLEVAFL FPWAVSFKEM GWFGFWSMMV FLLVLTVGFI YEWKKGALEW N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sinorhizobium meliloti mutant strain SP10 which is impaired in stationary phase survival shows a reduction in the energy charge due to its defect in the energy-conserving NADH dehydrogenase." Schmidt R., Uhde C., Nagel A., Puehler A., Selbitschka W. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RCR2011 / SU47.
[2]	"Rhizobium meliloti carries two sets of nuo genes." Putnoky P., Jady B., Chellapilla K.P., Barta F., Kiss E. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[3]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF055637 Genomic DNA. Translation: AAC12754.1. AJ245398 Genomic DNA. Translation: CAB51620.1. AL591688 Genomic DNA. Translation: CAC45843.1.
RefSeq	NP_385370.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1232912.
GenomeReviews	Gene locus R01264 in contig AL591688_GR.
KEGG	sme:SMc01912.
NMPDR	fig|266834.1.peg.2558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O68852.
OMA	O68852. IGFAYEW.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01912-MON.
BRENDA	1.6.99.5. 142.
Family and domain databases
HAMAP	MF_01394. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA1_RHIME

Accession

Primary (citable) accession number: O68852

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)