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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

outO

Organism
Vibrio cholerae
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.UniRule annotation

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: TIGR
  • protein secretion Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, MethyltransferaseUniRule annotationImported, ProteaseUniRule annotation, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzymeUniRule annotation (EC:2.1.1.-UniRule annotation, EC:3.4.23.43UniRule annotation)
Gene namesi
Name:outOImported
Synonyms:pilDImported
ORF Names:EN12_11765Imported, ERS013138_03101Imported, ERS013140_00632Imported, ERS013199_02129Imported, ERS013202_00444Imported, ERS013206_00688Imported, ERS013208_00947Imported
OrganismiVibrio choleraeImported
Taxonomic identifieri666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000039011 Componenti: Unassembled WGS sequence
  • UP000043247 Componenti: Unassembled WGS sequence
  • UP000040416 Componenti: Unassembled WGS sequence
  • UP000045183 Componenti: Unassembled WGS sequence
  • UP000045578 Componenti: Unassembled WGS sequence
  • UP000042589 Componenti: Unassembled WGS sequence
  • UP000036184 Componenti: Chromosome 1

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 3025HelicalSequence analysisAdd
BLAST
Transmembranei112 – 14534HelicalSequence analysisAdd
BLAST
Transmembranei157 – 17418HelicalSequence analysisAdd
BLAST
Transmembranei181 – 19919HelicalSequence analysisAdd
BLAST
Transmembranei219 – 24527HelicalSequence analysisAdd
BLAST
Transmembranei257 – 27519HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Interactioni

Protein-protein interaction databases

STRINGi345073.VC0395_A2002.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 124108DiS_P_DiSInterPro annotationAdd
BLAST
Domaini135 – 244110Peptidase_A24InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A24 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
KOiK02654.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

O68839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELFYFYPWL FPVLATLFGL IVGSFLNVVI YRLPKIMERE WRAECAASFP
60 70 80 90 100
EYGITPPEGK LTLSLPRSTC PHCQTPIRVI DNIPLLSWLA LRGQCSHCKA
110 120 130 140 150
PISARYPLIE LLTALMSLVI ATHFPFGVFA VALLFFSYVL IAATFIDFDT
160 170 180 190 200
LLLPDQLTLP LLWGGIALAL LGFSPVSLSD AVIGAMAGYL SLWSIYWLFK
210 220 230 240 250
LLTGKEGMGY GDFKLLAALG AWLGWQQLPV IVLLSSVVGV IFGLIQLRQQ
260 270 280 290
KKGIDMAFPF GPYLAIAGWF ALLWGDKVID WYFTTWVGQP L
Length:291
Mass (Da):32,434
Last modified:August 1, 1998 - v1
Checksum:i9A1ECD8D730A6EEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055371 Genomic DNA. Translation: AAC63504.1.
AF109904 Genomic DNA. Translation: AAD21032.1.
CP007653 Genomic DNA. Translation: AKO75893.1.
CWQG01000001 Genomic DNA. Translation: CSB54571.1.
CWQD01000002 Genomic DNA. Translation: CSB72863.1.
CWRE01000011 Genomic DNA. Translation: CSC51491.1.
CWQX01000025 Genomic DNA. Translation: CSC60610.1.
CWRC01000094 Genomic DNA. Translation: CSD35635.1.
CWSS01000144 Genomic DNA. Translation: CSI44827.1.
PIRiB82078.
RefSeqiWP_000418747.1. NZ_LRBH01000003.1.

Genome annotation databases

EnsemblBacteriaiAKO75893; AKO75893; EN12_11765.
CRZ38046; CRZ38046; ERS013242_00069.
CRZ40169; CRZ40169; ERS013276_00607.
CRZ44002; CRZ44002; ERS013128_01120.
CRZ45410; CRZ45410; ERS013250_01449.
CRZ62522; CRZ62522; ERS013182_00640.
CRZ62531; CRZ62531; ERS013125_00599.
CRZ63426; CRZ63426; ERS013130_00609.
CRZ70638; CRZ70638; ERS013171_00626.
CRZ71363; CRZ71363; ERS013180_00663.
CRZ75930; CRZ75930; ERS013135_00881.
CRZ78833; CRZ78833; ERS013163_00761.
CRZ79783; CRZ79783; ERS013162_00758.
CRZ81275; CRZ81275; ERS013179_00732.
CRZ82356; CRZ82356; ERS013160_00923.
CRZ85196; CRZ85196; ERS013178_00717.
CRZ86621; CRZ86621; ERS013181_01088.
CRZ88592; CRZ88592; ERS013129_01134.
CRZ92147; CRZ92147; ERS013172_00729.
CRZ92302; CRZ92302; ERS013131_00846.
CRZ98406; CRZ98406; ERS013132_00069.
CSA08160; CSA08160; ERS013126_01339.
CSA08800; CSA08800; ERS013254_01103.
CSA11740; CSA11740; ERS013175_01278.
CSA12391; CSA12391; ERS013170_01140.
CSA14000; CSA14000; ERS013169_01459.
CSA17709; CSA17709; ERS013177_01690.
CSA18172; CSA18172; ERS013127_02123.
CSA18513; CSA18513; ERS013124_01635.
CSA26822; CSA26822; ERS013134_01932.
CSA39382; CSA39382; ERS013168_01283.
CSA42327; CSA42327; ERS013176_01899.
CSA49723; CSA49723; ERS013252_01780.
CSA51688; CSA51688; ERS013253_01680.
CSA60666; CSA60666; ERS013263_01761.
CSA62752; CSA62752; ERS013259_02191.
CSA62880; CSA62880; ERS013255_02287.
CSA64753; CSA64753; ERS013260_02531.
CSA73790; CSA73790; ERS013257_02467.
CSA73793; CSA73793; ERS013261_02495.
CSA75397; CSA75397; ERS013167_02545.
CSA75910; CSA75910; ERS013164_02255.
CSA75962; CSA75962; ERS013258_02383.
CSA81026; CSA81026; ERS013133_02474.
CSA85942; CSA85942; ERS013174_02629.
CSB25142; CSB25142; ERS013185_01023.
CSB25237; CSB25237; ERS013195_00662.
CSB25570; CSB25570; ERS013161_03274.
CSB30548; CSB30548; ERS013256_02565.
CSB41730; CSB41730; ERS013191_01360.
CSB54571; CSB54571; ERS013202_00444.
CSB58955; CSB58955; ERS013136_00923.
CSB72863; CSB72863; ERS013206_00688.
CSB75532; CSB75532; ERS013189_02824.
CSB79533; CSB79533; ERS013218_01450.
CSB87687; CSB87687; ERS013146_01570.
CSB93943; CSB93943; ERS013147_01313.
CSC00627; CSC00627; ERS013188_02210.
CSC01594; CSC01594; ERS013196_02933.
CSC09737; CSC09737; ERS013139_01797.
CSC10040; CSC10040; ERS013213_01207.
CSC18276; CSC18276; ERS013145_02746.
CSC20765; CSC20765; ERS013205_02087.
CSC25959; CSC25959; ERS013210_01819.
CSC32527; CSC32527; ERS013190_03122.
CSC35517; CSC35517; ERS013217_02128.
CSC39074; CSC39074; ERS013137_01956.
CSC44798; CSC44798; ERS013143_02633.
CSC49178; CSC49178; ERS013216_01683.
CSC49541; CSC49541; ERS013142_02013.
CSC51491; CSC51491; ERS013140_00632.
CSC60610; CSC60610; ERS013199_02129.
CSC63685; CSC63685; ERS013226_01612.
CSC67161; CSC67161; ERS013212_03144.
CSC70280; CSC70280; ERS013141_02343.
CSC72253; CSC72253; ERS013209_03458.
CSC73292; CSC73292; ERS013221_02046.
CSC78293; CSC78293; ERS013225_01523.
CSC79050; CSC79050; ERS013224_01464.
CSC80486; CSC80486; ERS013211_02436.
CSC85245; CSC85245; ERS013273_00069.
CSC90829; CSC90829; ERS013265_01298.
CSC93773; CSC93773; ERS013266_00799.
CSC97242; CSC97242; ERS013268_00799.
CSC99627; CSC99627; ERS013241_00614.
CSD00868; CSD00868; ERS013232_00618.
CSD12146; CSD12146; ERS013267_01347.
CSD18905; CSD18905; ERS013244_00069.
CSD20797; CSD20797; ERS013251_00069.
CSD24090; CSD24090; ERS013233_00897.
CSD24753; CSD24753; ERS013245_00069.
CSD32873; CSD32873; ERS013275_00069.
CSD34221; CSD34221; ERS013271_01555.
CSD35635; CSD35635; ERS013138_03101.
CSD38125; CSD38125; ERS013234_01845.
CSD44068; CSD44068; ERS013243_00753.
CSD50530; CSD50530; ERS013269_02140.
CSD50606; CSD50606; ERS013274_00610.
CSD50840; CSD50840; ERS013238_01818.
CSD51364; CSD51364; ERS013236_02042.
CSD51602; CSD51602; ERS013246_00813.
CSD56074; CSD56074; ERS013237_02159.
CSD58129; CSD58129; ERS013282_00164.
CSD60612; CSD60612; ERS013235_02295.
CSD62634; CSD62634; ERS013248_01036.
CSD63333; CSD63333; ERS013247_01363.
CSD63824; CSD63824; ERS013278_00657.
CSD64515; CSD64515; ERS013270_02549.
CSD73334; CSD73334; ERS013239_01913.
CSD75644; CSD75644; ERS013240_01786.
CSD78708; CSD78708; ERS013249_01475.
CSD97552; CSD97552; ERS013279_01759.
CSE16924; CSE16924; ERS013281_02499.
CSI44827; CSI44827; ERS013208_00947.
KEGGivcq:EN18_15165.
PATRICi20083869. VBIVibCho83274_2309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055371 Genomic DNA. Translation: AAC63504.1.
AF109904 Genomic DNA. Translation: AAD21032.1.
CP007653 Genomic DNA. Translation: AKO75893.1.
CWQG01000001 Genomic DNA. Translation: CSB54571.1.
CWQD01000002 Genomic DNA. Translation: CSB72863.1.
CWRE01000011 Genomic DNA. Translation: CSC51491.1.
CWQX01000025 Genomic DNA. Translation: CSC60610.1.
CWRC01000094 Genomic DNA. Translation: CSD35635.1.
CWSS01000144 Genomic DNA. Translation: CSI44827.1.
PIRiB82078.
RefSeqiWP_000418747.1. NZ_LRBH01000003.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A2002.

Protocols and materials databases

DNASUi2612968.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAKO75893; AKO75893; EN12_11765.
CRZ38046; CRZ38046; ERS013242_00069.
CRZ40169; CRZ40169; ERS013276_00607.
CRZ44002; CRZ44002; ERS013128_01120.
CRZ45410; CRZ45410; ERS013250_01449.
CRZ62522; CRZ62522; ERS013182_00640.
CRZ62531; CRZ62531; ERS013125_00599.
CRZ63426; CRZ63426; ERS013130_00609.
CRZ70638; CRZ70638; ERS013171_00626.
CRZ71363; CRZ71363; ERS013180_00663.
CRZ75930; CRZ75930; ERS013135_00881.
CRZ78833; CRZ78833; ERS013163_00761.
CRZ79783; CRZ79783; ERS013162_00758.
CRZ81275; CRZ81275; ERS013179_00732.
CRZ82356; CRZ82356; ERS013160_00923.
CRZ85196; CRZ85196; ERS013178_00717.
CRZ86621; CRZ86621; ERS013181_01088.
CRZ88592; CRZ88592; ERS013129_01134.
CRZ92147; CRZ92147; ERS013172_00729.
CRZ92302; CRZ92302; ERS013131_00846.
CRZ98406; CRZ98406; ERS013132_00069.
CSA08160; CSA08160; ERS013126_01339.
CSA08800; CSA08800; ERS013254_01103.
CSA11740; CSA11740; ERS013175_01278.
CSA12391; CSA12391; ERS013170_01140.
CSA14000; CSA14000; ERS013169_01459.
CSA17709; CSA17709; ERS013177_01690.
CSA18172; CSA18172; ERS013127_02123.
CSA18513; CSA18513; ERS013124_01635.
CSA26822; CSA26822; ERS013134_01932.
CSA39382; CSA39382; ERS013168_01283.
CSA42327; CSA42327; ERS013176_01899.
CSA49723; CSA49723; ERS013252_01780.
CSA51688; CSA51688; ERS013253_01680.
CSA60666; CSA60666; ERS013263_01761.
CSA62752; CSA62752; ERS013259_02191.
CSA62880; CSA62880; ERS013255_02287.
CSA64753; CSA64753; ERS013260_02531.
CSA73790; CSA73790; ERS013257_02467.
CSA73793; CSA73793; ERS013261_02495.
CSA75397; CSA75397; ERS013167_02545.
CSA75910; CSA75910; ERS013164_02255.
CSA75962; CSA75962; ERS013258_02383.
CSA81026; CSA81026; ERS013133_02474.
CSA85942; CSA85942; ERS013174_02629.
CSB25142; CSB25142; ERS013185_01023.
CSB25237; CSB25237; ERS013195_00662.
CSB25570; CSB25570; ERS013161_03274.
CSB30548; CSB30548; ERS013256_02565.
CSB41730; CSB41730; ERS013191_01360.
CSB54571; CSB54571; ERS013202_00444.
CSB58955; CSB58955; ERS013136_00923.
CSB72863; CSB72863; ERS013206_00688.
CSB75532; CSB75532; ERS013189_02824.
CSB79533; CSB79533; ERS013218_01450.
CSB87687; CSB87687; ERS013146_01570.
CSB93943; CSB93943; ERS013147_01313.
CSC00627; CSC00627; ERS013188_02210.
CSC01594; CSC01594; ERS013196_02933.
CSC09737; CSC09737; ERS013139_01797.
CSC10040; CSC10040; ERS013213_01207.
CSC18276; CSC18276; ERS013145_02746.
CSC20765; CSC20765; ERS013205_02087.
CSC25959; CSC25959; ERS013210_01819.
CSC32527; CSC32527; ERS013190_03122.
CSC35517; CSC35517; ERS013217_02128.
CSC39074; CSC39074; ERS013137_01956.
CSC44798; CSC44798; ERS013143_02633.
CSC49178; CSC49178; ERS013216_01683.
CSC49541; CSC49541; ERS013142_02013.
CSC51491; CSC51491; ERS013140_00632.
CSC60610; CSC60610; ERS013199_02129.
CSC63685; CSC63685; ERS013226_01612.
CSC67161; CSC67161; ERS013212_03144.
CSC70280; CSC70280; ERS013141_02343.
CSC72253; CSC72253; ERS013209_03458.
CSC73292; CSC73292; ERS013221_02046.
CSC78293; CSC78293; ERS013225_01523.
CSC79050; CSC79050; ERS013224_01464.
CSC80486; CSC80486; ERS013211_02436.
CSC85245; CSC85245; ERS013273_00069.
CSC90829; CSC90829; ERS013265_01298.
CSC93773; CSC93773; ERS013266_00799.
CSC97242; CSC97242; ERS013268_00799.
CSC99627; CSC99627; ERS013241_00614.
CSD00868; CSD00868; ERS013232_00618.
CSD12146; CSD12146; ERS013267_01347.
CSD18905; CSD18905; ERS013244_00069.
CSD20797; CSD20797; ERS013251_00069.
CSD24090; CSD24090; ERS013233_00897.
CSD24753; CSD24753; ERS013245_00069.
CSD32873; CSD32873; ERS013275_00069.
CSD34221; CSD34221; ERS013271_01555.
CSD35635; CSD35635; ERS013138_03101.
CSD38125; CSD38125; ERS013234_01845.
CSD44068; CSD44068; ERS013243_00753.
CSD50530; CSD50530; ERS013269_02140.
CSD50606; CSD50606; ERS013274_00610.
CSD50840; CSD50840; ERS013238_01818.
CSD51364; CSD51364; ERS013236_02042.
CSD51602; CSD51602; ERS013246_00813.
CSD56074; CSD56074; ERS013237_02159.
CSD58129; CSD58129; ERS013282_00164.
CSD60612; CSD60612; ERS013235_02295.
CSD62634; CSD62634; ERS013248_01036.
CSD63333; CSD63333; ERS013247_01363.
CSD63824; CSD63824; ERS013278_00657.
CSD64515; CSD64515; ERS013270_02549.
CSD73334; CSD73334; ERS013239_01913.
CSD75644; CSD75644; ERS013240_01786.
CSD78708; CSD78708; ERS013249_01475.
CSD97552; CSD97552; ERS013279_01759.
CSE16924; CSE16924; ERS013281_02499.
CSI44827; CSI44827; ERS013208_00947.
KEGGivcq:EN18_15165.
PATRICi20083869. VBIVibCho83274_2309.

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
KOiK02654.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the Vibrio cholerae type 4 prepilin peptidase required for cholera toxin secretion and pilus formation."
    Marsh J.W., Taylor R.K.
    Mol. Microbiol. 29:1481-1492(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C6706str2Imported.
  2. "Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae."
    Fullner K.J., Mekalanos J.J.
    Infect. Immun. 67:1393-1404(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TSY216Imported.
  4. Informatics Pathogen
    Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 6180Imported, A346Imported, A346(2)Imported, A481Imported, A487Imported, A487(2)Imported, GP106Imported and PRL18Imported.

Entry informationi

Entry nameiO68839_VIBCL
AccessioniPrimary (citable) accession number: O68839
Secondary accession number(s): Q7DCT3
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.