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Protein

Cytosol aminopeptidase

Gene

pepA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi266 – 2661Manganese 2By similarity
Metal bindingi271 – 2711Manganese 1By similarity
Metal bindingi271 – 2711Manganese 2By similarity
Active sitei278 – 2781Sequence analysis
Metal bindingi289 – 2891Manganese 2By similarity
Metal bindingi348 – 3481Manganese 1By similarity
Metal bindingi350 – 3501Manganese 1By similarity
Metal bindingi350 – 3501Manganese 2By similarity
Active sitei352 – 3521Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • RNA metabolic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Synonyms:phpA
Ordered Locus Names:PA3831
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3831.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Cytosol aminopeptidasePRO_0000165782Add
BLAST

Proteomic databases

PaxDbiO68822.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3831.

Structurei

3D structure databases

ProteinModelPortaliO68822.
SMRiO68822. Positions 1-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243132.
InParanoidiO68822.
KOiK01255.
OMAiFIGACEN.
PhylomeDBiO68822.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFLVKSVRP ETLKTATLVL AVGEGRKLGA SAKAVDDATG GAISAVLKRG
60 70 80 90 100
DLAGKVGQTL LLQSLPNLKA ERVLLVGAGK ERELGDRQYR KLASAVLSTL
110 120 130 140 150
KGLAGADAAL ALGDLAVKGR DAHAKARLLV ETLADGLYVF DRYKSQKAEP
160 170 180 190 200
LKLKKLTLLA DKADSAAVEQ GSKEAQAIAN GMALTRDLGN LPPNVCHPTF
210 220 230 240 250
LGEQAKGLAK EFKGLKVEVL DEKKLRELGM GSFLAVAQGS DQPPRLIVLQ
260 270 280 290 300
YNGAKKDQAP HVLVGKGITF DTGGISLKPG LGMDEMKFDM CGAASVFGTF
310 320 330 340 350
RAVLELQLPI NLVGLLACAE NMPSGGATRP GDIVTTMSGQ TVEILNTDAE
360 370 380 390 400
GRLVLCDALT YAERFKPQSV IDIATLTGAC IVALGSNTSG LMGNNEALVR
410 420 430 440 450
QLLKAGEFAD DRAWQLPLFD EYQEQLDSPF ADIANIGGPK AGTITAGCFL
460 470 480 490
SRFAKKYHWA HLDIAGTAWI SGGKDKGATG RPVPLLTQYL LERAK
Length:495
Mass (Da):52,332
Last modified:August 1, 1998 - v1
Checksum:iE08C7928E7A0FCDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054622 Genomic DNA. Translation: AAD04821.1.
AE004091 Genomic DNA. Translation: AAG07218.1.
PIRiD83167.
RefSeqiNP_252520.1. NC_002516.2.
WP_003092867.1. NZ_ASJY01000612.1.

Genome annotation databases

EnsemblBacteriaiAAG07218; AAG07218; PA3831.
GeneIDi879865.
KEGGipae:PA3831.
PATRICi19842309. VBIPseAer58763_4011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054622 Genomic DNA. Translation: AAD04821.1.
AE004091 Genomic DNA. Translation: AAG07218.1.
PIRiD83167.
RefSeqiNP_252520.1. NC_002516.2.
WP_003092867.1. NZ_ASJY01000612.1.

3D structure databases

ProteinModelPortaliO68822.
SMRiO68822. Positions 1-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3831.

Proteomic databases

PaxDbiO68822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07218; AAG07218; PA3831.
GeneIDi879865.
KEGGipae:PA3831.
PATRICi19842309. VBIPseAer58763_4011.

Organism-specific databases

PseudoCAPiPA3831.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243132.
InParanoidiO68822.
KOiK01255.
OMAiFIGACEN.
PhylomeDBiO68822.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_PSEAE
AccessioniPrimary (citable) accession number: O68822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.