O68799 (MOBA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Molybdenum cofactor guanylyltransferase
Short name=MoCo guanylyltransferase
EC=2.7.7.77

Alternative name(s):
GTP:molybdopterin guanylyltransferase
Mo-MPT guanylyltransferase
Molybdopterin guanylyltransferase
Molybdopterin-guanine dinucleotide synthase
Short name=MGD synthase

Gene names

Name:	mobA
Ordered Locus Names:	PA3030

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor By similarity. HAMAP-Rule MF_00316
Catalytic activity	GTP + molybdenum cofactor = diphosphate + guanylyl molybdenum cofactor. HAMAP-Rule MF_00316
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00316
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00316
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00316.
Domain	The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein By similarity. HAMAP-Rule MF_00316
Sequence similarities	Belongs to the MobA family.

Ontologies

Keywords
Biological process	Molybdenum cofactor biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP guanylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Molybdenum cofactor guanylyltransferase HAMAP-Rule MF_00316		PRO_0000134899
Regions
	Nucleotide binding	14 – 16	3	GTP By similarity
Sites
	Metal binding	103	1	Magnesium By similarity
	Binding site	27	1	GTP By similarity
	Binding site	73	1	GTP By similarity
	Binding site	103	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O68799 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6E2AE65FE3464BBA	FASTA	198	21,870
	10 20 30 40 50 60 MPDSALPPCS ILLLAGGRGQ RMGGRDKGLI EWQGLPLIAH LHRLVRPLTD DLIVSCNRNQ 70 80 90 100 110 120 ERYAAYADRV VSDDSRDFPG PLAGIRAGLA VARHPWLLVL PCDAPRIDRA LLETLLQAAG 130 140 150 160 170 180 RTPARPWMLR CGGQWEPLFS LIPTHLAEEI EHAWRQGDRS PRHVLLPLGA EAIELAAGDP 190 RLANLNTPEL LANHRELK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cytochrome c precursor gene in Pseudomonas aeruginosa." Kerschen J., Hassett D.J., Rowe J.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053982 Genomic DNA. Translation: AAC15714.1. AE004091 Genomic DNA. Translation: AAG06418.1.
PIR	E83266.
RefSeq	NP_251720.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O68799.
SMR	O68799. Positions 12-192.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA3030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882980.
KEGG	pae:PA3030.
PATRIC	19840619. VBIPseAer58763_3179.
Organism-specific databases
PseudoCAP	PA3030.
Phylogenomic databases
eggNOG	COG0746.
HOGENOM	HOG000280423.
KO	K03752.
OMA	NTPELLS.
ProtClustDB	PRK00317.
Family and domain databases
HAMAP	MF_00316. MobA.
InterPro	IPR025877. MobA-like_NTP_Trfase_dom. IPR013482. Molybde_CF_guanTrfase. [Graphical view]
Pfam	PF12804. NTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02665. molyb_mobA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MOBA_PSEAE

Accession

Primary (citable) accession number: O68799

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program