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Protein

Cysteine protease YopT

Gene

yopT

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cysteine protease, which is translocated into infected cells and plays a central role in pathogenesis by cleaving the C-terminus end of the human small GTPase RhoA/ARHA, a regulator of cytoskeleton. Once cleaved, ARHA loses its lipid modification, and is released from the cell membrane, leading to the subsequent disruption of actin cytoskeleton of the host cell.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391
Active sitei258 – 2581
Active sitei274 – 2741

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciYPES214092:GKDD-4223-MONOMER.

Protein family/group databases

MEROPSiC58.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine protease YopT (EC:3.4.22.-)
Gene namesi
Name:yopT
Ordered Locus Names:YPCD1.20, y5059, y0065, YP_pCD67
Encoded oniPlasmid pCD10 Publication
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Plasmid pCD1
  • UP000001019 Componenti: Plasmid pCD1

Subcellular locationi

  • Secreted

  • Note: In infected cells, it is cytoplasmic. Translocated into the host cell by the type III secretion apparatus with the help of the SycT chaperone.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391C → S: Loss of function; abolishes the cleavage of ARHA. 1 Publication
Mutagenesisi146 – 1461W → A: Abolishes cytotoxicity. 1 Publication
Mutagenesisi165 – 1651R → A: No effect. 1 Publication
Mutagenesisi258 – 2581H → A: Loss of function; abolishes the cleavage of ARHA. 1 Publication
Mutagenesisi274 – 2741D → A: Loss of function; abolishes the cleavage of ARHA. 1 Publication
Mutagenesisi279 – 2791E → A: No effect. 1 Publication
Mutagenesisi300 – 3001S → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Cysteine protease YopTPRO_0000192513Add
BLAST

Interactioni

Subunit structurei

Interacts with human ARHA.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO68703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C58 family.Curated

Phylogenomic databases

HOGENOMiHOG000266867.
KOiK08599.
OMAiWLEVHAN.
OrthoDBiEOG6SR8Z4.

Family and domain databases

InterProiIPR003951. Peptidase_C58.
IPR006473. Peptidase_C58_Yopt.
[Graphical view]
PfamiPF03543. Peptidase_C58. 1 hit.
[Graphical view]
PRINTSiPR01376. BACSURFANTGN.
TIGRFAMsiTIGR01586. yopT_cys_prot. 1 hit.

Sequencei

Sequence statusi: Complete.

O68703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIHGHYHI QLSNYSAGEN LQSATLTEGV IGAHRVKVET ALSHSNLQKK
60 70 80 90 100
LSATIKHNQS GRSMLDRKLT SDGKANQRSS FTFSMIMYRM IHFVLSTRVP
110 120 130 140 150
AVRESVANYG GNINFKFAQT KGAFLHKIIK HSDTASGVCE ALCAHWIRSH
160 170 180 190 200
AQGQSLFDQL YVGGRKGKFQ IDTLYSIKQL QIDGCKADVD QDEVTLDWFK
210 220 230 240 250
KNGISERMIE RHCLLRPVDV TGTTESEGLD QLLNAILDTH GIGYGYKKIH
260 270 280 290 300
LSGQMSAHAI AAYVNEKSGV TFFDPNFGEF HFSDKEKFRK WFTNSFWGNS
310 320
MYHYPLGVGQ RFRVLTFDSK EV
Length:322
Mass (Da):36,308
Last modified:August 1, 1998 - v1
Checksum:i2B964F437FBC8A63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074612 Genomic DNA. Translation: AAC69833.1.
AF053946 Genomic DNA. Translation: AAC62582.1.
AL117189 Genomic DNA. Translation: CAB54897.1.
AE017043 Genomic DNA. Translation: AAS58582.1.
PIRiT43601.
RefSeqiNP_395155.1. NC_003131.1.
NP_857758.1. NC_004836.1.
NP_857958.1. NC_004839.1.
WP_002213006.1. NZ_LIXY01000017.1.

Genome annotation databases

EnsemblBacteriaiAAS58582; AAS58582; YP_pCD67.
GeneIDi1149322.
1172665.
15151811.
KEGGipg:1149322.
pg:15151811.
ype:YPCD1.20.
ypm:YP_pCD67.
PATRICi18588558. VBIYerPes7843_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074612 Genomic DNA. Translation: AAC69833.1.
AF053946 Genomic DNA. Translation: AAC62582.1.
AL117189 Genomic DNA. Translation: CAB54897.1.
AE017043 Genomic DNA. Translation: AAS58582.1.
PIRiT43601.
RefSeqiNP_395155.1. NC_003131.1.
NP_857758.1. NC_004836.1.
NP_857958.1. NC_004839.1.
WP_002213006.1. NZ_LIXY01000017.1.

3D structure databases

ProteinModelPortaliO68703.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC58.001.

Protocols and materials databases

DNASUi1149322.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS58582; AAS58582; YP_pCD67.
GeneIDi1149322.
1172665.
15151811.
KEGGipg:1149322.
pg:15151811.
ype:YPCD1.20.
ypm:YP_pCD67.
PATRICi18588558. VBIYerPes7843_0024.

Phylogenomic databases

HOGENOMiHOG000266867.
KOiK08599.
OMAiWLEVHAN.
OrthoDBiEOG6SR8Z4.

Enzyme and pathway databases

BioCyciYPES214092:GKDD-4223-MONOMER.

Family and domain databases

InterProiIPR003951. Peptidase_C58.
IPR006473. Peptidase_C58_Yopt.
[Graphical view]
PfamiPF03543. Peptidase_C58. 1 hit.
[Graphical view]
PRINTSiPR01376. BACSURFANTGN.
TIGRFAMsiTIGR01586. yopT_cys_prot. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing and analysis of the low-Ca2+-response plasmid pCD1 of Yersinia pestis KIM5."
    Perry R.D., Straley S.C., Fetherston J.D., Rose D.J., Gregor J., Blattner F.R.
    Infect. Immun. 66:4611-4623(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KIM5 / Biovar Mediaevalis.
  2. "Structural organization of virulence-associated plasmids of Yersinia pestis."
    Hu P., Elliott J., McCready P., Skowronski E., Garnes J., Kobayashi A., Brubaker R.R., Garcia E.
    J. Bacteriol. 180:5192-5202(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KIM5 / Biovar Mediaevalis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  4. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.
  5. "A Yersinia effector and a Pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis."
    Shao F., Merritt P.M., Bao Z., Innes R.W., Dixon J.E.
    Cell 109:575-588(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, FUNCTION, INTERACTION WITH HUMAN ARHA, MUTAGENESIS OF CYS-139; TRP-146; ARG-165; HIS-258; ASP-274; GLU-279 AND SER-300.

Entry informationi

Entry nameiYOPT_YERPE
AccessioniPrimary (citable) accession number: O68703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.