O68602 (HIS4_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	Cgl2096, cg2299

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.
Caution	Ala-129 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP tryptophan biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000142001
Sites
	Active site	10	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	82	1	G → S in AAC05575. Ref.1
	Sequence conflict	90 – 92	3	ERA → DTQ in AAC05575. Ref.1
	Sequence conflict	105	1	A → L in AAC05575. Ref.1
	Sequence conflict	119 – 127	9	RYGEKIAVD → AMARRLLS in AAC05575. Ref.1
	Sequence conflict	157	1	R → C in AAC05575. Ref.1
	Sequence conflict	195 – 198	4	PIVA → TYLT in AAC05575. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O68602 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: 6A35CF9153C32B1E	FASTA	246	26,609
	10 20 30 40 50 60 MTFTILPAVD VVNGQAVRLD QGEAGTEKSY GTPLESALKW QEQGAKWLHF VDLDAAFNRG 70 80 90 100 110 120 SNHEMMAEIV GKLDVDVELT GGIRDDESLE RALATGARRV NIGTAALEKP EWIASAIQRY 130 140 150 160 170 180 GEKIAVDIAV RLEDGEWRTR GNGWVSDGGD LWEVLERLDS QGCARFVVTD VSKDGTLSGP 190 200 210 220 230 240 NVELLREVAA ATDAPIVASG GISVLEDVLE LAKYQDEGID SVIIGKALYE HKFTLEEALA AVEKLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the histidine biosynthetic genes of Corynebacterium glutamicum: organization and sequencing analysis of the hisA, impA, and hisF gene cluster." Jung S.-I., Han M.-S., Kwon J.-H., Cheon C.-I., Min K.-H., Lee M.-S. Biochem. Biophys. Res. Commun. 247:741-745(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF051846 Genomic DNA. Translation: AAC05575.1. BA000036 Genomic DNA. Translation: BAB99489.1. BX927154 Genomic DNA. Translation: CAF20432.1.
PIR	JE0213.
RefSeq	NP_601295.1. NC_003450.3. YP_226333.1. NC_006958.1.
3D structure databases
ProteinModelPortal	O68602.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg2299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB99489; BAB99489; BAB99489. CAF20432; CAF20432; cg2299.
GeneID	1020047. 3344802.
KEGG	cgb:cg2299. cgl:NCgl2015.
PATRIC	21496192. VBICorGlu203724_2032.
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	CARYVVT.
ProtClustDB	PRK14024.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-2069-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA. Divergent sequence.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR010188. HisA_TrpF. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR01919. hisA-trpF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_CORGL

Accession

Primary (citable) accession number: O68602

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	July 26, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program