O68584 (T2H3_HAEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HaeIII
Short name=R.HaeIII
EC=3.1.21.4

Alternative name(s):
Endonuclease HaeIII
Type II restriction enzyme HaeIII

Gene names

Name:

haeIIIR

Organism

Haemophilus aegyptius

Taxonomic identifier

197575 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGCC and cleaves after G-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Type-2 restriction enzyme HaeIII		PRO_0000077317

Sequences

	Sequence		Length	Mass (Da)
	O68584 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 10E35B4B51F977DD	FASTA	318	37,129
	10 20 30 40 50 60 MSNKSNDNGR AYEFAFINEL GRIATQNQNI NIEKNSSYYV VEKSWSTLSD LEKEKYTKSA 70 80 90 100 110 120 IAGINLITSL EPIIEDGNGV LNLKIQADNK GELGDIRDIL IQRENINWEI GLSLKHNHFA 130 140 150 160 170 180 VKHSRLSHKI DFSEKWFQLP SSQNYWDNIL PIFEKLEIYK KDKIKWRELS NKEDCIYYPI 190 200 210 220 230 240 LKSFIAEIKE KYDKYNSIVP QRMVEYLLGY FDFYKIISQD NKKLTSIQSF NLRGTLNKPS 250 260 270 280 290 300 KKRKADIFIP VANLPTRIID IDFKPNSKNT VELYLDKGWQ FSFRIHNAST IIEPSLKFDI 310 KLIGVPATII CLETPWEE

References

[1]	Zhang B.-H., Wilson G.G. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF051375 Genomic DNA. Translation: AAC05697.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1089. HaeIII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019059. Restrct_endonuc_II_HaeIII. [Graphical view]
Pfam	PF09556. RE_HaeIII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2H3_HAEAE

Accession

Primary (citable) accession number: O68584

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	July 11, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program