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O68584 (T2H3_HAEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HaeIII

Short name=R.HaeIII
EC=3.1.21.4
Alternative name(s):
Endonuclease HaeIII
Type II restriction enzyme HaeIII
Gene names
Name:haeIIIR
OrganismHaemophilus aegyptius
Taxonomic identifier197575 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGCC and cleaves after G-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Type-2 restriction enzyme HaeIII
PRO_0000077317

Sequences

Sequence LengthMass (Da)Tools
O68584 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 10E35B4B51F977DD

FASTA31837,129
        10         20         30         40         50         60 
MSNKSNDNGR AYEFAFINEL GRIATQNQNI NIEKNSSYYV VEKSWSTLSD LEKEKYTKSA 

        70         80         90        100        110        120 
IAGINLITSL EPIIEDGNGV LNLKIQADNK GELGDIRDIL IQRENINWEI GLSLKHNHFA 

       130        140        150        160        170        180 
VKHSRLSHKI DFSEKWFQLP SSQNYWDNIL PIFEKLEIYK KDKIKWRELS NKEDCIYYPI 

       190        200        210        220        230        240 
LKSFIAEIKE KYDKYNSIVP QRMVEYLLGY FDFYKIISQD NKKLTSIQSF NLRGTLNKPS 

       250        260        270        280        290        300 
KKRKADIFIP VANLPTRIID IDFKPNSKNT VELYLDKGWQ FSFRIHNAST IIEPSLKFDI 

       310 
KLIGVPATII CLETPWEE 

« Hide

References

[1]Zhang B.-H., Wilson G.G.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF051375 Genomic DNA. Translation: AAC05697.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1089. HaeIII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019059. Restrct_endonuc_II_HaeIII.
[Graphical view]
PfamPF09556. RE_HaeIII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2H3_HAEAE
AccessionPrimary (citable) accession number: O68584
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries