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Protein

Pyruvate formate-lyase-activating enzyme

Gene

act

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi41Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi44Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei87S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei215S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme
Gene namesi
Name:act
Synonyms:pflA, pflC
Ordered Locus Names:SMU_1692
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002512 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005331 – 263Pyruvate formate-lyase-activating enzymeAdd BLAST263

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_1692.

Structurei

3D structure databases

ProteinModelPortaliO68575.
SMRiO68575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 45S-adenosyl-L-methionine bindingBy similarity3
Regioni142 – 144S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
KOiK04069.
OMAiIGVPNKR.
PhylomeDBiO68575.

Family and domain databases

InterProiView protein in InterPro
IPR034457. Organic_radical-activating.
IPR012839. Organic_radical_activase.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
SFLDiSFLDG01066. organic_radical-activating_enz. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiView protein in PROSITE
PS01087. RADICAL_ACTIVATING. 1 hit.

Sequencei

Sequence statusi: Complete.

O68575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKVDYEKV TGLVNSTESF GSVDGPGIRF VVFMQGCQMR CQYCHNPDTW
60 70 80 90 100
AMKNDRATER TAGDVFKEAL RFKDFWGDTG GITVSGGEAT LQMDFLIALF
110 120 130 140 150
SLAKEKGIHT TLDTCALTFR NTPKYLEKYE KLMAVTDLVL LDIKEINPDQ
160 170 180 190 200
HKIVTGHSNK TILACARYLS DIGKPVWIRH VLVPGLTDRD EDLIKLGEYV
210 220 230 240 250
KTLKNVQRFE ILPYHTMGEF KWRELGIPYP LEGVKPPTPD RVRNAKKLMH
260
TETYEEYKKR INH
Length:263
Mass (Da):30,150
Last modified:August 1, 1998 - v1
Checksum:i575405F3EE248EFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051356 Genomic DNA. Translation: AAC05773.1.
AB018417 Genomic DNA. Translation: BAA34998.1.
AE014133 Genomic DNA. Translation: AAN59329.1.
RefSeqiNP_722023.1. NC_004350.2.
WP_002262580.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN59329; AAN59329; SMU_1692.
GeneIDi1028925.
KEGGismu:SMU_1692.
PATRICifig|210007.7.peg.1512.

Similar proteinsi

Entry informationi

Entry nameiPFLA_STRMU
AccessioniPrimary (citable) accession number: O68575
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: August 30, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families