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O68557 (T2B1_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BglI

Short name=R.BglI
EC=3.1.21.4
Alternative name(s):
Endonuclease BglI
Type II restriction enzyme BglI
Gene names
Name:bglIR
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GCCNNNNNGGC and cleaves before N-8.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Binds 2 magnesium ions per subunit.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Type-2 restriction enzyme BglI
PRO_0000077285

Sites

Metal binding1161Magnesium 1
Metal binding1161Magnesium 2
Metal binding1421Magnesium 1
Metal binding1431Magnesium 1; via carbonyl oxygen

Secondary structure

....................................................... 299
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O68557 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 40DF210E1173B02B

FASTA29934,014
        10         20         30         40         50         60 
MYNLHREKIF MSYNQNKQYL EDNPEIQEKI ELYGLNLLNE VISDNEEEIR ADYNEANFLH 

        70         80         90        100        110        120 
PFWMNYPPLD RGKMPKGDQI PWIEVGEKAV GSKLTRLVSQ REDITVREIG LPTGPDERYL 

       130        140        150        160        170        180 
LTSPTIYSLT NGFTDSIMMF VDIKSVGPRD SDYDLVLSPN QVSGNGDWAQ LEGGIQNNQQ 

       190        200        210        220        230        240 
TIQGPRSSQI FLPTIPPLYI LSDGTIAPVV HLFIKPIYAM RSLTKGDTGQ SLYKIKLASV 

       250        260        270        280        290 
PNGLGLFCNP GYAFDSAYKF LFRPGKDDRT KSLLQKRVRV DLRVLDKIGP RVMTIDMDK 

« Hide

References

[1]"Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
Strain: Globigii / RUB562.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF050216 Genomic DNA. Translation: AAC63973.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DMUX-ray2.20A1-299[»]
ProteinModelPortalO68557.
SMRO68557. Positions 1-299.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE260. BglI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.600.20. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR011543. Restrct_endonuc_II_BglI.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO68557.

Entry information

Entry nameT2B1_BACIU
AccessionPrimary (citable) accession number: O68557
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references