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O68557

- T2B1_BACIU

UniProt

O68557 - T2B1_BACIU

Protein

Type-2 restriction enzyme BglI

Gene

bglIR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (16 Apr 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GCCNNNNNGGC and cleaves before N-8.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi116 – 1161Magnesium 1
    Metal bindingi116 – 1161Magnesium 2
    Metal bindingi142 – 1421Magnesium 1
    Metal bindingi143 – 1431Magnesium 1; via carbonyl oxygen

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium, Metal-binding

    Protein family/group databases

    REBASEi260. BglI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BglI (EC:3.1.21.4)
    Short name:
    R.BglI
    Alternative name(s):
    Endonuclease BglI
    Type II restriction enzyme BglI
    Gene namesi
    Name:bglIR
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299Type-2 restriction enzyme BglIPRO_0000077285Add
    BLAST

    Structurei

    Secondary structure

    1
    299
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni2 – 54
    Helixi6 – 2217
    Helixi24 – 4421
    Helixi46 – 5813
    Helixi60 – 634
    Beta strandi72 – 743
    Helixi83 – 897
    Helixi91 – 999
    Beta strandi105 – 1073
    Beta strandi116 – 1227
    Helixi124 – 1296
    Turni130 – 1323
    Beta strandi135 – 1439
    Beta strandi154 – 1574
    Helixi159 – 1613
    Beta strandi180 – 1834
    Beta strandi188 – 1914
    Beta strandi197 – 2004
    Beta strandi206 – 22015
    Helixi221 – 2233
    Beta strandi230 – 24011
    Helixi243 – 2475
    Helixi252 – 2543
    Helixi256 – 2583
    Beta strandi261 – 2644
    Beta strandi278 – 2814
    Helixi282 – 2854
    Beta strandi288 – 2903
    Beta strandi292 – 2965

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DMUX-ray2.20A1-299[»]
    ProteinModelPortaliO68557.
    SMRiO68557. Positions 1-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO68557.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.600.20. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR011543. Restrct_endonuc_II_BglI.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O68557-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNLHREKIF MSYNQNKQYL EDNPEIQEKI ELYGLNLLNE VISDNEEEIR    50
    ADYNEANFLH PFWMNYPPLD RGKMPKGDQI PWIEVGEKAV GSKLTRLVSQ 100
    REDITVREIG LPTGPDERYL LTSPTIYSLT NGFTDSIMMF VDIKSVGPRD 150
    SDYDLVLSPN QVSGNGDWAQ LEGGIQNNQQ TIQGPRSSQI FLPTIPPLYI 200
    LSDGTIAPVV HLFIKPIYAM RSLTKGDTGQ SLYKIKLASV PNGLGLFCNP 250
    GYAFDSAYKF LFRPGKDDRT KSLLQKRVRV DLRVLDKIGP RVMTIDMDK 299
    Length:299
    Mass (Da):34,014
    Last modified:August 1, 1998 - v1
    Checksum:i40DF210E1173B02B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF050216 Genomic DNA. Translation: AAC63973.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF050216 Genomic DNA. Translation: AAC63973.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DMU X-ray 2.20 A 1-299 [» ]
    ProteinModelPortali O68557.
    SMRi O68557. Positions 1-299.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 260. BglI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O68557.

    Family and domain databases

    Gene3Di 3.40.600.20. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR011543. Restrct_endonuc_II_BglI.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
      Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
      EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
      Strain: Globigii / RUB562.

    Entry informationi

    Entry nameiT2B1_BACIU
    AccessioniPrimary (citable) accession number: O68557
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: August 1, 1998
    Last modified: April 16, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3