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O68557

- T2B1_BACIU

UniProt

O68557 - T2B1_BACIU

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Protein

Type-2 restriction enzyme BglI

Gene

bglIR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GCCNNNNNGGC and cleaves before N-8.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Magnesium 1
Metal bindingi116 – 1161Magnesium 2
Metal bindingi142 – 1421Magnesium 1
Metal bindingi143 – 1431Magnesium 1; via carbonyl oxygen

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi260. BglI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BglI (EC:3.1.21.4)
Short name:
R.BglI
Alternative name(s):
Endonuclease BglI
Type II restriction enzyme BglI
Gene namesi
Name:bglIR
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Type-2 restriction enzyme BglIPRO_0000077285Add
BLAST

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni2 – 54
Helixi6 – 2217
Helixi24 – 4421
Helixi46 – 5813
Helixi60 – 634
Beta strandi72 – 743
Helixi83 – 897
Helixi91 – 999
Beta strandi105 – 1073
Beta strandi116 – 1227
Helixi124 – 1296
Turni130 – 1323
Beta strandi135 – 1439
Beta strandi154 – 1574
Helixi159 – 1613
Beta strandi180 – 1834
Beta strandi188 – 1914
Beta strandi197 – 2004
Beta strandi206 – 22015
Helixi221 – 2233
Beta strandi230 – 24011
Helixi243 – 2475
Helixi252 – 2543
Helixi256 – 2583
Beta strandi261 – 2644
Beta strandi278 – 2814
Helixi282 – 2854
Beta strandi288 – 2903
Beta strandi292 – 2965

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DMUX-ray2.20A1-299[»]
ProteinModelPortaliO68557.
SMRiO68557. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO68557.

Family & Domainsi

Family and domain databases

Gene3Di3.40.600.20. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011543. Restrct_endonuc_II_BglI.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

O68557-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNLHREKIF MSYNQNKQYL EDNPEIQEKI ELYGLNLLNE VISDNEEEIR
60 70 80 90 100
ADYNEANFLH PFWMNYPPLD RGKMPKGDQI PWIEVGEKAV GSKLTRLVSQ
110 120 130 140 150
REDITVREIG LPTGPDERYL LTSPTIYSLT NGFTDSIMMF VDIKSVGPRD
160 170 180 190 200
SDYDLVLSPN QVSGNGDWAQ LEGGIQNNQQ TIQGPRSSQI FLPTIPPLYI
210 220 230 240 250
LSDGTIAPVV HLFIKPIYAM RSLTKGDTGQ SLYKIKLASV PNGLGLFCNP
260 270 280 290
GYAFDSAYKF LFRPGKDDRT KSLLQKRVRV DLRVLDKIGP RVMTIDMDK
Length:299
Mass (Da):34,014
Last modified:August 1, 1998 - v1
Checksum:i40DF210E1173B02B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF050216 Genomic DNA. Translation: AAC63973.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF050216 Genomic DNA. Translation: AAC63973.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DMU X-ray 2.20 A 1-299 [» ]
ProteinModelPortali O68557.
SMRi O68557. Positions 1-299.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 260. BglI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei O68557.

Family and domain databases

Gene3Di 3.40.600.20. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR011543. Restrct_endonuc_II_BglI.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
    Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
    EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
    Strain: Globigii / RUB562.

Entry informationi

Entry nameiT2B1_BACIU
AccessioniPrimary (citable) accession number: O68557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3