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Protein

Type-2 restriction enzyme BglI

Gene

bglIR

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GCCNNNNNGGC and cleaves before N-8.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Magnesium 11
Metal bindingi116Magnesium 21
Metal bindingi142Magnesium 11
Metal bindingi143Magnesium 1; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi260. BglI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BglI (EC:3.1.21.4)
Short name:
R.BglI
Alternative name(s):
Endonuclease BglI
Type II restriction enzyme BglI
Gene namesi
Name:bglIR
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772851 – 299Type-2 restriction enzyme BglIAdd BLAST299

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni2 – 5Combined sources4
Helixi6 – 22Combined sources17
Helixi24 – 44Combined sources21
Helixi46 – 58Combined sources13
Helixi60 – 63Combined sources4
Beta strandi72 – 74Combined sources3
Helixi83 – 89Combined sources7
Helixi91 – 99Combined sources9
Beta strandi105 – 107Combined sources3
Beta strandi116 – 122Combined sources7
Helixi124 – 129Combined sources6
Turni130 – 132Combined sources3
Beta strandi135 – 143Combined sources9
Beta strandi154 – 157Combined sources4
Helixi159 – 161Combined sources3
Beta strandi180 – 183Combined sources4
Beta strandi188 – 191Combined sources4
Beta strandi197 – 200Combined sources4
Beta strandi206 – 220Combined sources15
Helixi221 – 223Combined sources3
Beta strandi230 – 240Combined sources11
Helixi243 – 247Combined sources5
Helixi252 – 254Combined sources3
Helixi256 – 258Combined sources3
Beta strandi261 – 264Combined sources4
Beta strandi278 – 281Combined sources4
Helixi282 – 285Combined sources4
Beta strandi288 – 290Combined sources3
Beta strandi292 – 296Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DMUX-ray2.20A1-299[»]
ProteinModelPortaliO68557.
SMRiO68557.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO68557.

Family & Domainsi

Family and domain databases

Gene3Di3.40.600.20. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011543. Restrct_endonuc_II_BglI.
[Graphical view]
PfamiPF14562. Endonuc_BglI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

O68557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNLHREKIF MSYNQNKQYL EDNPEIQEKI ELYGLNLLNE VISDNEEEIR
60 70 80 90 100
ADYNEANFLH PFWMNYPPLD RGKMPKGDQI PWIEVGEKAV GSKLTRLVSQ
110 120 130 140 150
REDITVREIG LPTGPDERYL LTSPTIYSLT NGFTDSIMMF VDIKSVGPRD
160 170 180 190 200
SDYDLVLSPN QVSGNGDWAQ LEGGIQNNQQ TIQGPRSSQI FLPTIPPLYI
210 220 230 240 250
LSDGTIAPVV HLFIKPIYAM RSLTKGDTGQ SLYKIKLASV PNGLGLFCNP
260 270 280 290
GYAFDSAYKF LFRPGKDDRT KSLLQKRVRV DLRVLDKIGP RVMTIDMDK
Length:299
Mass (Da):34,014
Last modified:August 1, 1998 - v1
Checksum:i40DF210E1173B02B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF050216 Genomic DNA. Translation: AAC63973.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF050216 Genomic DNA. Translation: AAC63973.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DMUX-ray2.20A1-299[»]
ProteinModelPortaliO68557.
SMRiO68557.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi260. BglI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO68557.

Family and domain databases

Gene3Di3.40.600.20. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011543. Restrct_endonuc_II_BglI.
[Graphical view]
PfamiPF14562. Endonuc_BglI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2B1_BACIU
AccessioniPrimary (citable) accession number: O68557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.