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O68556 (MTB1_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BglI

Short name=M.BglI
EC=2.1.1.113
Alternative name(s):
BglI modification methyltransferase
N(4)- cytosine-specific methyltransferase BglI
Gene names
Name:bglIM
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCCNNNNNGGC, causes specific methylation on a C on both strands, and protects the DNA from cleavage by the BglI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Modification methylase BglI
PRO_0000087923

Sequences

Sequence LengthMass (Da)Tools
O68556 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 7B08004E0DB7E707

FASTA34840,242
        10         20         30         40         50         60 
MNNHSYLKKN SFHEGDAREL LKCIEEESIA LSVWSPPYHV GKKYEEGQTY EQWSSLLTKV 

        70         80         90        100        110        120 
IALHYPILKP GGFLVINIDD ILAFPDPRMP RFQAVNLKKH RVSVTREDIL NALKLEPELT 

       130        140        150        160        170        180 
KYQLAKKFNC SEQTIERRLK GNNIRGGKYN VQTKVKLAGP VLEKAAEEAG LYLYDRRIWA 

       190        200        210        220        230        240 
KDPAWQNSQW HSNSYKAVSE FEHLYIFWKP GETVIDRNKL SKEEWASWAS RGIWYIPSVR 

       250        260        270        280        290        300 
KNDDHEAKFP LLLPQRLIKL LTQKGDTVLD CFMGSGTTAV AALSESRNFI GIEKEPKYIQ 

       310        320        330        340 
LSNKNVETFY ISRNKEASKI KETNHSVTDE KKKAEQLELL LEENENSL 

« Hide

References

[1]"Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF050216 Genomic DNA. Translation: AAC63974.1.

3D structure databases

ProteinModelPortalO68556.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3303. M.BglI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR002941. DNA_methylase_N4/N6.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
ProtoNetSearch...

Entry information

Entry nameMTB1_BACIU
AccessionPrimary (citable) accession number: O68556
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries