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O68556

- MTB1_BACIU

UniProt

O68556 - MTB1_BACIU

Protein

Modification methylase BglI

Gene

bglIM

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GCCNNNNNGGC, causes specific methylation on a C on both strands, and protects the DNA from cleavage by the BglI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (cytosine-N4-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3303. M.BglI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BglI (EC:2.1.1.113)
    Short name:
    M.BglI
    Alternative name(s):
    BglI modification methyltransferase
    N(4)- cytosine-specific methyltransferase BglI
    Gene namesi
    Name:bglIM
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Modification methylase BglIPRO_0000087923Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO68556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O68556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNHSYLKKN SFHEGDAREL LKCIEEESIA LSVWSPPYHV GKKYEEGQTY    50
    EQWSSLLTKV IALHYPILKP GGFLVINIDD ILAFPDPRMP RFQAVNLKKH 100
    RVSVTREDIL NALKLEPELT KYQLAKKFNC SEQTIERRLK GNNIRGGKYN 150
    VQTKVKLAGP VLEKAAEEAG LYLYDRRIWA KDPAWQNSQW HSNSYKAVSE 200
    FEHLYIFWKP GETVIDRNKL SKEEWASWAS RGIWYIPSVR KNDDHEAKFP 250
    LLLPQRLIKL LTQKGDTVLD CFMGSGTTAV AALSESRNFI GIEKEPKYIQ 300
    LSNKNVETFY ISRNKEASKI KETNHSVTDE KKKAEQLELL LEENENSL 348
    Length:348
    Mass (Da):40,242
    Last modified:August 1, 1998 - v1
    Checksum:i7B08004E0DB7E707
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF050216 Genomic DNA. Translation: AAC63974.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF050216 Genomic DNA. Translation: AAC63974.1 .

    3D structure databases

    ProteinModelPortali O68556.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3303. M.BglI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
      Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
      EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTB1_BACIU
    AccessioniPrimary (citable) accession number: O68556
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3