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O68556

- MTB1_BACIU

UniProt

O68556 - MTB1_BACIU

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Protein

Modification methylase BglI

Gene

bglIM

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCCNNNNNGGC, causes specific methylation on a C on both strands, and protects the DNA from cleavage by the BglI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (cytosine-N4-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3303. M.BglI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BglI (EC:2.1.1.113)
Short name:
M.BglI
Alternative name(s):
BglI modification methyltransferase
N(4)- cytosine-specific methyltransferase BglI
Gene namesi
Name:bglIM
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Modification methylase BglIPRO_0000087923Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO68556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequencei

Sequence statusi: Complete.

O68556 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNHSYLKKN SFHEGDAREL LKCIEEESIA LSVWSPPYHV GKKYEEGQTY
60 70 80 90 100
EQWSSLLTKV IALHYPILKP GGFLVINIDD ILAFPDPRMP RFQAVNLKKH
110 120 130 140 150
RVSVTREDIL NALKLEPELT KYQLAKKFNC SEQTIERRLK GNNIRGGKYN
160 170 180 190 200
VQTKVKLAGP VLEKAAEEAG LYLYDRRIWA KDPAWQNSQW HSNSYKAVSE
210 220 230 240 250
FEHLYIFWKP GETVIDRNKL SKEEWASWAS RGIWYIPSVR KNDDHEAKFP
260 270 280 290 300
LLLPQRLIKL LTQKGDTVLD CFMGSGTTAV AALSESRNFI GIEKEPKYIQ
310 320 330 340
LSNKNVETFY ISRNKEASKI KETNHSVTDE KKKAEQLELL LEENENSL
Length:348
Mass (Da):40,242
Last modified:August 1, 1998 - v1
Checksum:i7B08004E0DB7E707
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF050216 Genomic DNA. Translation: AAC63974.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF050216 Genomic DNA. Translation: AAC63974.1 .

3D structure databases

ProteinModelPortali O68556.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3303. M.BglI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence."
    Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V.
    EMBO J. 17:5466-5476(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTB1_BACIU
AccessioniPrimary (citable) accession number: O68556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3