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O68460 (HPPA_RHORT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Gene names
Name:hppA
Synonyms:rrpP, vppA
Ordered Locus Names:Rru_A1818
OrganismRhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)
Taxonomic identifier269796 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01129

Cofactor

Magnesium By similarity. HAMAP MF_01129

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 702702K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129
PRO_0000217030

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane63 – 8321Helical; Potential
Transmembrane130 – 15021Helical; Potential
Transmembrane162 – 18221Helical; Potential
Transmembrane234 – 25421Helical; Potential
Transmembrane255 – 27521Helical; Potential
Transmembrane294 – 31421Helical; Potential
Transmembrane329 – 34921Helical; Potential
Transmembrane379 – 39921Helical; Potential
Transmembrane407 – 42721Helical; Potential
Transmembrane466 – 48621Helical; Potential
Transmembrane517 – 53721Helical; Potential
Transmembrane586 – 60621Helical; Potential
Transmembrane612 – 63221Helical; Potential

Sites

Site4651Determinant of potassium independence By similarity

Experimental info

Mutagenesis1851G → A: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. Ref.4
Mutagenesis2221C → V: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. Ref.4
Mutagenesis5731C → A: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. Ref.4
Sequence conflict721L → V in CAD70568. Ref.3
Sequence conflict781G → V in CAD70568. Ref.3
Sequence conflict1851G → C in AAC38615. Ref.1
Sequence conflict4761A → S in AAC38615. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O68460 [UniParc].

Last modified March 21, 2006. Version 3.
Checksum: 6F0D50BEA919F0F2

FASTA70271,547
        10         20         30         40         50         60 
MAGIYLFVVA AALAALGYGA LTIKTIMAAD AGTARMQEIS GAVQEGASAF LNRQYKTIAV 

        70         80         90        100        110        120 
VGAVVFVILT ALLGISVGFG FLIGAVCSGI AGYVGMYISV RANVRVAAGA QQGLARGLEL 

       130        140        150        160        170        180 
AFQSGAVTGM LVAGLALLSV AFYYILLVGI GATGRALIDP LVALGFGASL ISIFARLGGG 

       190        200        210        220        230        240 
IFTKGADVGA DLVGKVEAGI PEDDPRNPAV IADNVGDNVG DCAGMAADLF ETYAVTVVAT 

       250        260        270        280        290        300 
MVLASIFFAG VPAMTSMMAY PLAIGGVCIL ASILGTKFVK LGPKNNIMGA LYRGFLVSAG 

       310        320        330        340        350        360 
ASFVGIILAT AIVPGFGDIQ GANGVLYSGF DLFLCAVIGL LVTGLLIWVT EYYTGTNFRP 

       370        380        390        400        410        420 
VRSVAKASTT GHGTNVIQGL AISMEATALP ALIICAAIIT TYQLSGLFGI AITVTSMLAL 

       430        440        450        460        470        480 
AGMVVALDAY GPVTDNAGGI AEMANLPEDV RKTTDALDAV GNTTKAVTKG YAIGSAGLGA 

       490        500        510        520        530        540 
LVLFAAYTED LAFFKANVDA YPAFAGVDVN FSLSSPYVVV GLFIGGLLPY LFGSMGMTAV 

       550        560        570        580        590        600 
GRAAGSVVEE VRRQFREIPG IMEGTAKPEY GRCVDMLTKA AIKEMIIPSL LPVLAPIVLY 

       610        620        630        640        650        660 
FVILGIADKS AAFSALGAML LGVIVTGLFV AISMTAGGGA WDNAKKYIED GHYGGKGSEA 

       670        680        690        700 
HKAAVTGDTV GDPYKDTAGP AVNPMIKITN IVALLLLAVL AH

« Hide

References

« Hide 'large scale' references
[1]"A pyrophosphate synthase gene: molecular cloning and sequencing of the cDNA encoding the inorganic pyrophosphate synthase from Rhodospirillum rubrum."
Baltscheffsky M., Nadanaciva S., Schultz A.
Biochim. Biophys. Acta 1364:301-306(1998) [PubMed: 9630689] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of the chromosome of Rhodospirillum rubrum ATCC 11170."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Mavrommatis K., Richardson P., Zhang Y., Roberts G., Reslewic S., Zhou S., Schwartz D.C.
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 11170 / NCIB 8255.
[3]"Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics."
Lopez-Marques R.L., Perez-Castineira J.R., Losada M., Serrano A.
J. Bacteriol. 186:5418-5426(2004) [PubMed: 15292143] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81.
[4]"H+-pyrophosphatase of Rhodospirillum rubrum. High yield expression in Escherichia coli and identification of the Cys residues responsible for inactivation my mersalyl."
Belogurov G.A., Turkina M.V., Penttinen A., Huopalahti S., Baykov A.A., Lahti R.
J. Biol. Chem. 277:22209-22214(2002) [PubMed: 11956221] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF GLY-185; CYS-222 AND CYS-573.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF044912 mRNA. Translation: AAC38615.2.
CP000230 Genomic DNA. Translation: ABC22618.1.
AJ549291 Genomic DNA. Translation: CAD70568.1.
RefSeqYP_426905.1. NC_007643.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO68460.

Protein family/group databases

TCDB3.A.10.2.1. H+-translocating pyrophosphatase (H+-PPase) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3835242.
GenomeReviewsGene locus Rru_A1818 in contig CP000230_GR.
KEGGrru:Rru_A1818.
NMPDRfig|1085.1.peg.2393.
PATRIC23327298. VBIRhoRub82919_1896.

Phylogenomic databases

eggNOGCOG3808.
HOGENOMHBG593668.
OMAAINRGFF.
PhylomeDBO68460.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycRRUB269796:RRU_A1818-MONOMER.
BRENDA3.6.1.1. 5420.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA_RHORT
AccessionPrimary (citable) accession number: O68460
Secondary accession number(s): Q2RTC7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 21, 2006
Last modified: January 25, 2012
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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