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Protein

K(+)-insensitive pyrophosphate-energized proton pump

Gene

hppA

Organism
Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei176 – 1761Important for ion transportBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Metal bindingi187 – 1871Magnesium 1By similarity
Metal bindingi191 – 1911Magnesium 1By similarity
Metal bindingi214 – 2141Magnesium 2By similarity
Metal bindingi217 – 2171Magnesium 2By similarity
Sitei221 – 2211Important for ion transportBy similarity
Sitei228 – 2281Important for ion transportBy similarity
Metal bindingi435 – 4351Magnesium 2By similarity
Sitei465 – 4651Important for potassium independenceBy similarity
Metal bindingi642 – 6421Calcium or magnesiumBy similarity
Metal bindingi668 – 6681Calcium or magnesiumBy similarity
Metal bindingi672 – 6721Calcium or magnesiumBy similarity
Binding sitei675 – 6751SubstrateBy similarity
Sitei676 – 6761Important for ion transportBy similarity
Sitei687 – 6871Important for ion transportBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRRUB269796:GCN1-1850-MONOMER.
BRENDAi3.6.1.1. 5420.

Protein family/group databases

TCDBi3.A.10.2.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-insensitive pyrophosphate-energized proton pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound proton-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
H(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Synonyms:rrpP, vppA
Ordered Locus Names:Rru_A1818
OrganismiRhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
Taxonomic identifieri269796 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeRhodospirillum
Proteomesi
  • UP000001929 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalUniRule annotationAdd
BLAST
Transmembranei63 – 8321HelicalUniRule annotationAdd
BLAST
Transmembranei130 – 15021HelicalUniRule annotationAdd
BLAST
Transmembranei162 – 18221HelicalUniRule annotationAdd
BLAST
Transmembranei234 – 25421HelicalUniRule annotationAdd
BLAST
Transmembranei255 – 27521HelicalUniRule annotationAdd
BLAST
Transmembranei294 – 31421HelicalUniRule annotationAdd
BLAST
Transmembranei329 – 34921HelicalUniRule annotationAdd
BLAST
Transmembranei379 – 39921HelicalUniRule annotationAdd
BLAST
Transmembranei407 – 42721HelicalUniRule annotationAdd
BLAST
Transmembranei466 – 48621HelicalUniRule annotationAdd
BLAST
Transmembranei517 – 53721HelicalUniRule annotationAdd
BLAST
Transmembranei586 – 60621HelicalUniRule annotationAdd
BLAST
Transmembranei612 – 63221HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi185 – 1851G → A: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. 1 Publication
Mutagenesisi222 – 2221C → V: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. 1 Publication
Mutagenesisi573 – 5731C → A: No effect on activity; decreased sensitivity to the sulfhydryl modifying reagent mersalyl. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702K(+)-insensitive pyrophosphate-energized proton pumpPRO_0000217030Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi269796.Rru_A1818.

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

O68460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIYLFVVA AALAALGYGA LTIKTIMAAD AGTARMQEIS GAVQEGASAF
60 70 80 90 100
LNRQYKTIAV VGAVVFVILT ALLGISVGFG FLIGAVCSGI AGYVGMYISV
110 120 130 140 150
RANVRVAAGA QQGLARGLEL AFQSGAVTGM LVAGLALLSV AFYYILLVGI
160 170 180 190 200
GATGRALIDP LVALGFGASL ISIFARLGGG IFTKGADVGA DLVGKVEAGI
210 220 230 240 250
PEDDPRNPAV IADNVGDNVG DCAGMAADLF ETYAVTVVAT MVLASIFFAG
260 270 280 290 300
VPAMTSMMAY PLAIGGVCIL ASILGTKFVK LGPKNNIMGA LYRGFLVSAG
310 320 330 340 350
ASFVGIILAT AIVPGFGDIQ GANGVLYSGF DLFLCAVIGL LVTGLLIWVT
360 370 380 390 400
EYYTGTNFRP VRSVAKASTT GHGTNVIQGL AISMEATALP ALIICAAIIT
410 420 430 440 450
TYQLSGLFGI AITVTSMLAL AGMVVALDAY GPVTDNAGGI AEMANLPEDV
460 470 480 490 500
RKTTDALDAV GNTTKAVTKG YAIGSAGLGA LVLFAAYTED LAFFKANVDA
510 520 530 540 550
YPAFAGVDVN FSLSSPYVVV GLFIGGLLPY LFGSMGMTAV GRAAGSVVEE
560 570 580 590 600
VRRQFREIPG IMEGTAKPEY GRCVDMLTKA AIKEMIIPSL LPVLAPIVLY
610 620 630 640 650
FVILGIADKS AAFSALGAML LGVIVTGLFV AISMTAGGGA WDNAKKYIED
660 670 680 690 700
GHYGGKGSEA HKAAVTGDTV GDPYKDTAGP AVNPMIKITN IVALLLLAVL

AH
Length:702
Mass (Da):71,547
Last modified:March 21, 2006 - v3
Checksum:i6F0D50BEA919F0F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721L → V in CAD70568 (PubMed:15292143).Curated
Sequence conflicti78 – 781G → V in CAD70568 (PubMed:15292143).Curated
Sequence conflicti185 – 1851G → C in AAC38615 (PubMed:9630689).Curated
Sequence conflicti476 – 4761A → S in AAC38615 (PubMed:9630689).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044912 mRNA. Translation: AAC38615.2.
CP000230 Genomic DNA. Translation: ABC22618.1.
AJ549291 Genomic DNA. Translation: CAD70568.1.
RefSeqiWP_011389571.1. NC_007643.1.
YP_426905.1. NC_007643.1.

Genome annotation databases

EnsemblBacteriaiABC22618; ABC22618; Rru_A1818.
GeneIDi3835242.
KEGGirru:Rru_A1818.
PATRICi23327298. VBIRhoRub82919_1896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044912 mRNA. Translation: AAC38615.2.
CP000230 Genomic DNA. Translation: ABC22618.1.
AJ549291 Genomic DNA. Translation: CAD70568.1.
RefSeqiWP_011389571.1. NC_007643.1.
YP_426905.1. NC_007643.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269796.Rru_A1818.

Protein family/group databases

TCDBi3.A.10.2.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC22618; ABC22618; Rru_A1818.
GeneIDi3835242.
KEGGirru:Rru_A1818.
PATRICi23327298. VBIRhoRub82919_1896.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Enzyme and pathway databases

BioCyciRRUB269796:GCN1-1850-MONOMER.
BRENDAi3.6.1.1. 5420.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA_RHORT
AccessioniPrimary (citable) accession number: O68460
Secondary accession number(s): Q2RTC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 21, 2006
Last modified: September 7, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.