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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

pilD

Organism
Legionella pneumophila
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the TapA pilin precursor during membrane translocation.

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

TCDBi3.A.15.3.1. the outer membrane protein secreting main terminal branch (mtb) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:pilD
OrganismiLegionella pneumophila
Taxonomic identifieri446 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001926221 – 287Type 4 prepilin-like proteins leader peptide-processing enzymeAdd BLAST287

Interactioni

Protein-protein interaction databases

STRINGi297246.lpp1481.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

O68433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINALIINYP WFMYLVVGLF SLAVGSLLNV IIYRLPIILQ EEWKEQCCEL
60 70 80 90 100
FHFEQRKEKI KLNLFLPRSF CPHCKAMVKA WQNIPLLAIL VLRGRCYQCD
110 120 130 140 150
SPFSIRYPFV ETLTTVLSLY ASWHFGFTIQ LLFALLAIWI LISLVFIDLD
160 170 180 190 200
HQLLPDSLTL GLLWIGLIAN TQNVFVSLDV AVLSCAGAYL ALWLFINLFY
210 220 230 240 250
LMTCKVCMGH GDFKLFAAFG AWLGWMYLPI ILLISSITGA IIGLIYLKIN
260 270 280
GKSRDTAIPF GPFLCISGLI AMFWGDSIIN WYIGYWM
Length:287
Mass (Da):32,850
Last modified:August 1, 1998 - v1
Checksum:i3DA1B1968498EB66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038655 Genomic DNA. Translation: AAC12718.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038655 Genomic DNA. Translation: AAC12718.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi297246.lpp1481.

Protein family/group databases

TCDBi3.A.15.3.1. the outer membrane protein secreting main terminal branch (mtb) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiLEP4_LEGPN
AccessioniPrimary (citable) accession number: O68433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.