Reviewed, UniProtKB/Swiss-Prot O68427 (TRPC_BUCDN)

Names and origin

Protein names

Recommended name:
    Tryptophan biosynthesis protein trpCF
Including the following 2 domains:
    1- Recommended name:
            Indole-3-glycerol phosphate synthase
                Short name=IGPS
              EC=4.1.1.48
    2- Recommended name:
            N-(5'-phospho-ribosyl)anthranilate isomerase
                Short name=PRAI
              EC=5.3.1.24

Gene names

Name:	trpC
Synonyms:	trpC/F

Organism

Buchnera aphidicola subsp. Diuraphis noxia

Taxonomic identifier

118101 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain. HAMAP MF_00134
Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP MF_00134 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP MF_00134
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP MF_00134 Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.
Sequence similarities	In the N-terminal section; belongs to the trpC family. In the C-terminal section; belongs to the trpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Decarboxylase Isomerase Lyase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	indole-3-glycerol-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Tryptophan biosynthesis protein trpCF HAMAP MF_00134		PRO_0000154274
Regions
	Region	1 – 257	257	Indole-3-glycerol phosphate synthase HAMAP MF_00134
	Region	258 – 453	196	N-(5'-phosphoribosyl)anthranilate isomerase HAMAP MF_00134

Sequences

	Sequence		Length	Mass (Da)
	O68427-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 4ED27374695024A5	FASTA	453	52,108
	10 20 30 40 50 60 MKETILEKIV KNKYEWIKFR KKKQPLITFK NHINTKTRNF YNSLKEKNPV FILECKKKSP 70 80 90 100 110 120 SLGIIKKNFN LIDIAKIYNK YASAISVLTD EKYFDGKLEF INIVRERVSQ PILCKDFFID 130 140 150 160 170 180 PFQIYLARYY NADAILLMLS ILDDFQYQKL SKIAKELNMG ILTEVNNTSE LKRAIKLNAN 190 200 210 220 230 240 IIGINNRNLH DLSINLNRTR ILSSLIPKNI IIISESGITK YKQIRYLSQF VNGFLIGSHL 250 260 270 280 290 300 MSEKQLEIGV RSLILGENKI CGLTRSCDIE IAEKYGAVYG GLIFAPSSLR KISKNTAKKI 310 320 330 340 350 360 IFNNTLRNVG VFQNENIEIV KNIAEELNLY AVQLHGQEDQ KYVKKLRQTL SINIQIWKAF 370 380 390 400 410 420 SIDSKIPDLN WDHIHKYVLD SQFGGSNKCF NWSILKHQIL ENVILAGGIN SNNCIKASKL 430 440 450 NCSGLDLNSG IEVSPGIKDH KKIKSVFQKL RYY

References

[1]	"The endosymbiont (Buchnera) of the aphid Diuraphis noxia contains all the genes of the tryptophan biosynthetic pathway." Baumann L., Baumann P., Moran N.A. Curr. Microbiol. 37:58-59(1998) [PubMed: 9625791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038565 Genomic DNA. Translation: AAC27734.1.
3D structure databases
HSSP	HSSP built from PDB template 1JCM based on UniProtKB P00909.
ModBase	Search...
Family and domain databases
HAMAP	MF_00134. Fused. [Tree] MF_00135. Fused. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR013798. Indole-3-glycerol_P_synth. IPR001468. Indole-3-GPS_central. IPR001240. PRAI. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00218. IGPS. 1 hit. PF00697. PRAI. 1 hit. [Graphical view]
ProDom	PD001511. IGPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00614. IGPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPC_BUCDN

Accession

Primary (citable) accession number: O68427

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	March 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)