O68283 (ALKD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxy-phosphogluconate aldolase
EC=4.1.2.14

Alternative name(s):
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase

Gene names

Name:	eda
Ordered Locus Names:	PA3181

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	2-dehydro-3-deoxy-phosphogluconate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	2-dehydro-3-deoxy-phosphogluconate aldolase		PRO_0000287743
Sites
	Active site	52	1	By similarity
	Active site	136	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O68283 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: E130639230FBA58F	FASTA	220	23,954
	10 20 30 40 50 60 MHNLEQKTAR IDTLCREARI LPVITIDREA DILPMADALA AGGLTALEIT LRTAHGLTAI 70 80 90 100 110 120 RRLSEERPHL RIGAGTVLDP RTFAAAEKAG ASFVVTPGCT DELLRFALDS EVPLLPGVAS 130 140 150 160 170 180 ASEIMLAYRH GYRRFKLFPA EVSGGPAALK AFSGPFPDIR FCPTGGVSLN NLADYLAVPN 190 200 210 220 VMCVGGTWML PKAVVDRGDW AQVERLSREA LERFAEHRRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"HexR, zwf, and eda genes." Hager P.W., Dail M.B., Phibbs P.V. Jr. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029673 Genomic DNA. Translation: AAC38312.2. AE004091 Genomic DNA. Translation: AAG06569.1.
PIR	G83247.
RefSeq	NP_251871.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O68283.
SMR	O68283. Positions 3-211.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA3181.
Protocols and materials databases
DNASU	882580.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882580.
KEGG	pae:PA3181.
PATRIC	19840929. VBIPseAer58763_3325.
Organism-specific databases
PseudoCAP	PA3181.
Phylogenomic databases
eggNOG	COG0800.
HOGENOM	HOG000233114.
KO	K01625.
OMA	PELCVGA.
ProtClustDB	PRK05718.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000887. Aldlse_KDPG_KHG. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF01081. Aldolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01182. eda. 1 hit.
PROSITE	PS00159. ALDOLASE_KDPG_KHG_1. 1 hit. PS00160. ALDOLASE_KDPG_KHG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKD_PSEAE

Accession

Primary (citable) accession number: O68283
Secondary accession number(s): Q7DCB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program