ID   G6PD_PSEAE              Reviewed;         489 AA.
AC   O68282;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Glucose-6-phosphate 1-dehydrogenase;
DE            Short=G6PD;
DE            EC=1.1.1.49;
GN   Name=zwf; OrderedLocusNames=PA3183;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=98196716; PubMed=9537370;
RA   Ma J.-F., Hager P.W., Howell M.L., Phibbs P.V. Jr., Hassett D.J.;
RT   "Cloning and characterization of the Pseudomonas aeruginosa zwf gene
RT   encoding glucose-6-phosphate dehydrogenase, an enzyme important in
RT   resistance to methyl viologen (paraquat).";
RL   J. Bacteriol. 180:1741-1749(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY: D-glucose 6-phosphate + NADP(+) = D-glucono-
CC       1,5-lactone 6-phosphate + NADPH.
CC   -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-
CC       ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage):
CC       step 1/3.
CC   -!- SIMILARITY: Belongs to the glucose-6-phosphate dehydrogenase
CC       family.
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DR   EMBL; AF029673; AAC38311.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG06571.1; -; Genomic_DNA.
DR   PIR; A83248; A83248.
DR   RefSeq; NP_251873.1; -.
DR   HSSP; P11413; 1QKI.
DR   GeneID; 882681; -.
DR   GenomeReviews; AE004091_GR; PA3183.
DR   KEGG; pae:PA3183; -.
DR   PseudoCAP; PA3183; -.
DR   HOGENOM; O68282; -.
DR   OMA; O68282; DGRSWYG.
DR   BioCyc; PAER208964:PA3183-MON; -.
DR   BRENDA; 1.1.1.49; 354.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004345; F:glucose-6-phosphate dehydrogenase activity; IEA:EC.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001282; Glc-6-P_DH.
DR   InterPro; IPR019796; Glc-6-P_DH_AS.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR23429; G6PDH; 1.
DR   Pfam; PF02781; G6PD_C; 1.
DR   Pfam; PF00479; G6PD_N; 1.
DR   PIRSF; PIRSF000110; G6PD; 1.
DR   PRINTS; PR00079; G6PDHDRGNASE.
DR   ProDom; PD001129; G6PD; 1.
DR   TIGRFAMs; TIGR00871; zwf; 1.
DR   PROSITE; PS00069; G6P_DEHYDROGENASE; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Complete proteome; Glucose metabolism; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    489       Glucose-6-phosphate 1-dehydrogenase.
FT                                /FTId=PRO_0000068131.
FT   ACT_SITE    237    237       Proton acceptor (By similarity).
FT   BINDING      17     17       NADP (By similarity).
FT   BINDING      49     49       NADP (By similarity).
FT   BINDING     175    175       Substrate (By similarity).
FT   BINDING     179    179       Substrate (By similarity).
FT   BINDING     342    342       Substrate (By similarity).
SQ   SEQUENCE   489 AA;  55620 MW;  755849EB33BF9486 CRC64;
     MPDVRVLPCT LALFGALGDL ALRKLFPALY QLDRENLLHR DTRVLALARD EGAPAEHLAT
     LEQRLRLAVP AKEWDDVVWQ RFRERLDYLS MDFLDPQAYV GLREAVDDEL PLVAYFATPA
     SVFGGICENL AAAGLAERTR VVLEKPIGHD LESSREVNEA VARFFPESRI YRIDHYLGKE
     TVQNLIALRF ANSLFETQWN QNHISHVEIT VAEKVGIEGR WGYFDQAGQL RDMVQNHLLQ
     LLCLIAMDPP SDLSADSIRD EKVKVLRALE PIPAEQLASR VVRGQYTAGF SDGKAVPGYL
     EEEHANRDSD AETFVALRVD IRNWRWSGVP FYLRTGKRMP QKLSQIVIHF KEPPHYIFAP
     EQRSLISNRL IIRLQPDEGI SLQVMTKDQG LGKGMQLRTG PLQLSFSETY HAARIPDAYE
     RLLLEVTQGN QYLFVRKDEV EFAWKWCDQL IAGWERLSEA PKPYPAGSWG PVASVALVAR
     DGRSWYGDF
//