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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP+ or NAD+.1 PublicationUniRule annotation

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.1 PublicationUniRule annotation

Kineticsi

  1. KM=530 µM for glucose 6-phosphate (with NADP)1 Publication
  2. KM=57 µM for NADP1 Publication
  3. KM=333 µM for NAD1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491NADPUniRule annotation
Binding sitei145 – 1451NADPUniRule annotation
Binding sitei175 – 1751SubstrateUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Binding sitei213 – 2131SubstrateUniRule annotation
Binding sitei232 – 2321SubstrateUniRule annotation
Active sitei237 – 2371Proton acceptorUniRule annotation
Binding sitei337 – 3371SubstrateUniRule annotation
Binding sitei342 – 3421SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 932NADPUniRule annotation

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. NADP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
Short name:
G6PDUniRule annotation
Gene namesi
Name:zwfUniRule annotation
Ordered Locus Names:PA3183
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3183.

Pathology & Biotechi

Disruption phenotypei

Mutant is unable to grow on minimal medium supplemented with mannitol and shows increased sensitivity to the redox-active superoxide-generating agent methyl viologen (paraquat).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Glucose-6-phosphate 1-dehydrogenasePRO_0000068131Add
BLAST

Expressioni

Inductioni

Transcription is maximal during early logarithmic phase when inducing substrates such as glycerol, glucose or gluconate are abundant. Transcription decreases during stationary phase.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA3183.

Structurei

3D structure databases

ProteinModelPortaliO68282.
SMRiO68282. Positions 11-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
InParanoidiO68282.
KOiK00036.
OMAiKSHIKTP.
OrthoDBiEOG61308Z.
PhylomeDBiO68282.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68282-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPDVRVLPCT LALFGALGDL ALRKLFPALY QLDRENLLHR DTRVLALARD
60 70 80 90 100
EGAPAEHLAT LEQRLRLAVP AKEWDDVVWQ RFRERLDYLS MDFLDPQAYV
110 120 130 140 150
GLREAVDDEL PLVAYFATPA SVFGGICENL AAAGLAERTR VVLEKPIGHD
160 170 180 190 200
LESSREVNEA VARFFPESRI YRIDHYLGKE TVQNLIALRF ANSLFETQWN
210 220 230 240 250
QNHISHVEIT VAEKVGIEGR WGYFDQAGQL RDMVQNHLLQ LLCLIAMDPP
260 270 280 290 300
SDLSADSIRD EKVKVLRALE PIPAEQLASR VVRGQYTAGF SDGKAVPGYL
310 320 330 340 350
EEEHANRDSD AETFVALRVD IRNWRWSGVP FYLRTGKRMP QKLSQIVIHF
360 370 380 390 400
KEPPHYIFAP EQRSLISNRL IIRLQPDEGI SLQVMTKDQG LGKGMQLRTG
410 420 430 440 450
PLQLSFSETY HAARIPDAYE RLLLEVTQGN QYLFVRKDEV EFAWKWCDQL
460 470 480
IAGWERLSEA PKPYPAGSWG PVASVALVAR DGRSWYGDF
Length:489
Mass (Da):55,620
Last modified:August 1, 1998 - v1
Checksum:i755849EB33BF9486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029673 Genomic DNA. Translation: AAC38311.1.
AE004091 Genomic DNA. Translation: AAG06571.1.
PIRiA83248.
RefSeqiNP_251873.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06571; AAG06571; PA3183.
GeneIDi882681.
KEGGipae:PA3183.
PATRICi19840933. VBIPseAer58763_3327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029673 Genomic DNA. Translation: AAC38311.1.
AE004091 Genomic DNA. Translation: AAG06571.1.
PIRiA83248.
RefSeqiNP_251873.1. NC_002516.2.

3D structure databases

ProteinModelPortaliO68282.
SMRiO68282. Positions 11-478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06571; AAG06571; PA3183.
GeneIDi882681.
KEGGipae:PA3183.
PATRICi19840933. VBIPseAer58763_3327.

Organism-specific databases

PseudoCAPiPA3183.

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
InParanoidiO68282.
KOiK00036.
OMAiKSHIKTP.
OrthoDBiEOG61308Z.
PhylomeDBiO68282.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat)."
    Ma J.-F., Hager P.W., Howell M.L., Phibbs P.V. Jr., Hassett D.J.
    J. Bacteriol. 180:1741-1749(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiG6PD_PSEAE
AccessioniPrimary (citable) accession number: O68282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.