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O68282

- G6PD_PSEAE

UniProt

O68282 - G6PD_PSEAE

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Protein
Glucose-6-phosphate 1-dehydrogenase
Gene
zwf, PA3183
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP+ or NAD+.1 Publication

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.1 Publication

Kineticsi

  1. KM=530 µM for glucose 6-phosphate (with NADP)1 Publication
  2. KM=57 µM for NADP
  3. KM=333 µM for NAD

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491NADP By similarity
Binding sitei145 – 1451NADP By similarity
Binding sitei175 – 1751Substrate By similarity
Binding sitei179 – 1791Substrate By similarity
Binding sitei213 – 2131Substrate By similarity
Binding sitei232 – 2321Substrate By similarity
Active sitei237 – 2371Proton acceptor By similarity
Binding sitei337 – 3371Substrate By similarity
Binding sitei342 – 3421Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 932NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: UniProtKB-HAMAP
  2. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:zwf
Ordered Locus Names:PA3183
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3183.

Pathology & Biotechi

Disruption phenotypei

Mutant is unable to grow on minimal medium supplemented with mannitol and shows increased sensitivity to the redox-active superoxide-generating agent methyl viologen (paraquat).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Glucose-6-phosphate 1-dehydrogenaseUniRule annotation
PRO_0000068131Add
BLAST

Expressioni

Inductioni

Transcription is maximal during early logarithmic phase when inducing substrates such as glycerol, glucose or gluconate are abundant. Transcription decreases during stationary phase.1 Publication

Interactioni

Subunit structurei

Homotetramer Inferred.1 Publication

Protein-protein interaction databases

STRINGi208964.PA3183.

Structurei

3D structure databases

ProteinModelPortaliO68282.
SMRiO68282. Positions 11-478.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
KOiK00036.
OMAiDSIMEAW.
OrthoDBiEOG61308Z.
PhylomeDBiO68282.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68282-1 [UniParc]FASTAAdd to Basket

« Hide

MPDVRVLPCT LALFGALGDL ALRKLFPALY QLDRENLLHR DTRVLALARD    50
EGAPAEHLAT LEQRLRLAVP AKEWDDVVWQ RFRERLDYLS MDFLDPQAYV 100
GLREAVDDEL PLVAYFATPA SVFGGICENL AAAGLAERTR VVLEKPIGHD 150
LESSREVNEA VARFFPESRI YRIDHYLGKE TVQNLIALRF ANSLFETQWN 200
QNHISHVEIT VAEKVGIEGR WGYFDQAGQL RDMVQNHLLQ LLCLIAMDPP 250
SDLSADSIRD EKVKVLRALE PIPAEQLASR VVRGQYTAGF SDGKAVPGYL 300
EEEHANRDSD AETFVALRVD IRNWRWSGVP FYLRTGKRMP QKLSQIVIHF 350
KEPPHYIFAP EQRSLISNRL IIRLQPDEGI SLQVMTKDQG LGKGMQLRTG 400
PLQLSFSETY HAARIPDAYE RLLLEVTQGN QYLFVRKDEV EFAWKWCDQL 450
IAGWERLSEA PKPYPAGSWG PVASVALVAR DGRSWYGDF 489
Length:489
Mass (Da):55,620
Last modified:August 1, 1998 - v1
Checksum:i755849EB33BF9486
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029673 Genomic DNA. Translation: AAC38311.1.
AE004091 Genomic DNA. Translation: AAG06571.1.
PIRiA83248.
RefSeqiNP_251873.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06571; AAG06571; PA3183.
GeneIDi882681.
KEGGipae:PA3183.
PATRICi19840933. VBIPseAer58763_3327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029673 Genomic DNA. Translation: AAC38311.1 .
AE004091 Genomic DNA. Translation: AAG06571.1 .
PIRi A83248.
RefSeqi NP_251873.1. NC_002516.2.

3D structure databases

ProteinModelPortali O68282.
SMRi O68282. Positions 11-478.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06571 ; AAG06571 ; PA3183 .
GeneIDi 882681.
KEGGi pae:PA3183.
PATRICi 19840933. VBIPseAer58763_3327.

Organism-specific databases

PseudoCAPi PA3183.

Phylogenomic databases

eggNOGi COG0364.
HOGENOMi HOG000046191.
KOi K00036.
OMAi DSIMEAW.
OrthoDBi EOG61308Z.
PhylomeDBi O68282.

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000110. G6PD. 1 hit.
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat)."
    Ma J.-F., Hager P.W., Howell M.L., Phibbs P.V. Jr., Hassett D.J.
    J. Bacteriol. 180:1741-1749(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiG6PD_PSEAE
AccessioniPrimary (citable) accession number: O68282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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