Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O68282

- G6PD_PSEAE

UniProt

O68282 - G6PD_PSEAE

Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP+ or NAD+.1 PublicationUniRule annotation

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.1 PublicationUniRule annotation

    Kineticsi

    1. KM=530 µM for glucose 6-phosphate (with NADP)1 Publication
    2. KM=57 µM for NADP1 Publication
    3. KM=333 µM for NAD1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491NADPUniRule annotation
    Binding sitei145 – 1451NADPUniRule annotation
    Binding sitei175 – 1751SubstrateUniRule annotation
    Binding sitei179 – 1791SubstrateUniRule annotation
    Binding sitei213 – 2131SubstrateUniRule annotation
    Binding sitei232 – 2321SubstrateUniRule annotation
    Active sitei237 – 2371Proton acceptorUniRule annotation
    Binding sitei337 – 3371SubstrateUniRule annotation
    Binding sitei342 – 3421SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi92 – 932NADPUniRule annotation

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. NADP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pentose-phosphate shunt Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
    Short name:
    G6PDUniRule annotation
    Gene namesi
    Name:zwfUniRule annotation
    Ordered Locus Names:PA3183
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3183.

    Pathology & Biotechi

    Disruption phenotypei

    Mutant is unable to grow on minimal medium supplemented with mannitol and shows increased sensitivity to the redox-active superoxide-generating agent methyl viologen (paraquat).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489Glucose-6-phosphate 1-dehydrogenasePRO_0000068131Add
    BLAST

    Expressioni

    Inductioni

    Transcription is maximal during early logarithmic phase when inducing substrates such as glycerol, glucose or gluconate are abundant. Transcription decreases during stationary phase.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3183.

    Structurei

    3D structure databases

    ProteinModelPortaliO68282.
    SMRiO68282. Positions 11-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046191.
    KOiK00036.
    OMAiDSIMEAW.
    OrthoDBiEOG61308Z.
    PhylomeDBiO68282.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O68282-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDVRVLPCT LALFGALGDL ALRKLFPALY QLDRENLLHR DTRVLALARD    50
    EGAPAEHLAT LEQRLRLAVP AKEWDDVVWQ RFRERLDYLS MDFLDPQAYV 100
    GLREAVDDEL PLVAYFATPA SVFGGICENL AAAGLAERTR VVLEKPIGHD 150
    LESSREVNEA VARFFPESRI YRIDHYLGKE TVQNLIALRF ANSLFETQWN 200
    QNHISHVEIT VAEKVGIEGR WGYFDQAGQL RDMVQNHLLQ LLCLIAMDPP 250
    SDLSADSIRD EKVKVLRALE PIPAEQLASR VVRGQYTAGF SDGKAVPGYL 300
    EEEHANRDSD AETFVALRVD IRNWRWSGVP FYLRTGKRMP QKLSQIVIHF 350
    KEPPHYIFAP EQRSLISNRL IIRLQPDEGI SLQVMTKDQG LGKGMQLRTG 400
    PLQLSFSETY HAARIPDAYE RLLLEVTQGN QYLFVRKDEV EFAWKWCDQL 450
    IAGWERLSEA PKPYPAGSWG PVASVALVAR DGRSWYGDF 489
    Length:489
    Mass (Da):55,620
    Last modified:August 1, 1998 - v1
    Checksum:i755849EB33BF9486
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029673 Genomic DNA. Translation: AAC38311.1.
    AE004091 Genomic DNA. Translation: AAG06571.1.
    PIRiA83248.
    RefSeqiNP_251873.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06571; AAG06571; PA3183.
    GeneIDi882681.
    KEGGipae:PA3183.
    PATRICi19840933. VBIPseAer58763_3327.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029673 Genomic DNA. Translation: AAC38311.1 .
    AE004091 Genomic DNA. Translation: AAG06571.1 .
    PIRi A83248.
    RefSeqi NP_251873.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali O68282.
    SMRi O68282. Positions 11-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06571 ; AAG06571 ; PA3183 .
    GeneIDi 882681.
    KEGGi pae:PA3183.
    PATRICi 19840933. VBIPseAer58763_3327.

    Organism-specific databases

    PseudoCAPi PA3183.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046191.
    KOi K00036.
    OMAi DSIMEAW.
    OrthoDBi EOG61308Z.
    PhylomeDBi O68282.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat)."
      Ma J.-F., Hager P.W., Howell M.L., Phibbs P.V. Jr., Hassett D.J.
      J. Bacteriol. 180:1741-1749(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiG6PD_PSEAE
    AccessioniPrimary (citable) accession number: O68282
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3