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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP+ or NAD+.UniRule annotation1 Publication

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation1 Publication

Kineticsi

  1. KM=530 µM for glucose 6-phosphate (with NADP)1 Publication
  2. KM=57 µM for NADP1 Publication
  3. KM=333 µM for NAD1 Publication

    Pathway:ipentose phosphate pathway

    This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).UniRule annotation
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Glucose-6-phosphate 1-dehydrogenase (zwf), Glucose-6-phosphate 1-dehydrogenase (zwf)
    2. 6-phosphogluconolactonase (pgl)
    3. no protein annotated in this organism
    This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491NADPUniRule annotation
    Binding sitei145 – 1451NADPUniRule annotation
    Binding sitei175 – 1751SubstrateUniRule annotation
    Binding sitei179 – 1791SubstrateUniRule annotation
    Binding sitei213 – 2131SubstrateUniRule annotation
    Binding sitei232 – 2321SubstrateUniRule annotation
    Active sitei237 – 2371Proton acceptorUniRule annotation
    Binding sitei337 – 3371SubstrateUniRule annotation
    Binding sitei342 – 3421SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi92 – 932NADPUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
    Short name:
    G6PDUniRule annotation
    Gene namesi
    Name:zwfUniRule annotation
    Ordered Locus Names:PA3183
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438 Componenti: Chromosome

    Organism-specific databases

    PseudoCAPiPA3183.

    Pathology & Biotechi

    Disruption phenotypei

    Mutant is unable to grow on minimal medium supplemented with mannitol and shows increased sensitivity to the redox-active superoxide-generating agent methyl viologen (paraquat).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489Glucose-6-phosphate 1-dehydrogenasePRO_0000068131Add
    BLAST

    Expressioni

    Inductioni

    Transcription is maximal during early logarithmic phase when inducing substrates such as glycerol, glucose or gluconate are abundant. Transcription decreases during stationary phase.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3183.

    Structurei

    3D structure databases

    ProteinModelPortaliO68282.
    SMRiO68282. Positions 11-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046191.
    InParanoidiO68282.
    KOiK00036.
    OMAiIDDWRWS.
    OrthoDBiEOG61308Z.
    PhylomeDBiO68282.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O68282-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPDVRVLPCT LALFGALGDL ALRKLFPALY QLDRENLLHR DTRVLALARD
    60 70 80 90 100
    EGAPAEHLAT LEQRLRLAVP AKEWDDVVWQ RFRERLDYLS MDFLDPQAYV
    110 120 130 140 150
    GLREAVDDEL PLVAYFATPA SVFGGICENL AAAGLAERTR VVLEKPIGHD
    160 170 180 190 200
    LESSREVNEA VARFFPESRI YRIDHYLGKE TVQNLIALRF ANSLFETQWN
    210 220 230 240 250
    QNHISHVEIT VAEKVGIEGR WGYFDQAGQL RDMVQNHLLQ LLCLIAMDPP
    260 270 280 290 300
    SDLSADSIRD EKVKVLRALE PIPAEQLASR VVRGQYTAGF SDGKAVPGYL
    310 320 330 340 350
    EEEHANRDSD AETFVALRVD IRNWRWSGVP FYLRTGKRMP QKLSQIVIHF
    360 370 380 390 400
    KEPPHYIFAP EQRSLISNRL IIRLQPDEGI SLQVMTKDQG LGKGMQLRTG
    410 420 430 440 450
    PLQLSFSETY HAARIPDAYE RLLLEVTQGN QYLFVRKDEV EFAWKWCDQL
    460 470 480
    IAGWERLSEA PKPYPAGSWG PVASVALVAR DGRSWYGDF
    Length:489
    Mass (Da):55,620
    Last modified:August 1, 1998 - v1
    Checksum:i755849EB33BF9486
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF029673 Genomic DNA. Translation: AAC38311.1.
    AE004091 Genomic DNA. Translation: AAG06571.1.
    PIRiA83248.
    RefSeqiNP_251873.1. NC_002516.2.
    WP_003113442.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06571; AAG06571; PA3183.
    GeneIDi882681.
    KEGGipae:PA3183.
    PATRICi19840933. VBIPseAer58763_3327.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF029673 Genomic DNA. Translation: AAC38311.1.
    AE004091 Genomic DNA. Translation: AAG06571.1.
    PIRiA83248.
    RefSeqiNP_251873.1. NC_002516.2.
    WP_003113442.1. NC_002516.2.

    3D structure databases

    ProteinModelPortaliO68282.
    SMRiO68282. Positions 11-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi208964.PA3183.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAG06571; AAG06571; PA3183.
    GeneIDi882681.
    KEGGipae:PA3183.
    PATRICi19840933. VBIPseAer58763_3327.

    Organism-specific databases

    PseudoCAPiPA3183.

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046191.
    InParanoidiO68282.
    KOiK00036.
    OMAiIDDWRWS.
    OrthoDBiEOG61308Z.
    PhylomeDBiO68282.

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat)."
      Ma J.-F., Hager P.W., Howell M.L., Phibbs P.V. Jr., Hassett D.J.
      J. Bacteriol. 180:1741-1749(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiG6PD_PSEAE
    AccessioniPrimary (citable) accession number: O68282
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: July 22, 2015
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.