Reviewed, UniProtKB/Swiss-Prot O68249 (RIBB_DEHMU)

Names and origin

Protein names

Recommended name:
    3,4-dihydroxy-2-butanone 4-phosphate synthase
      Short name=DHBP synthase
    EC=4.1.99.12

Gene names

Name:

ribB

Organism

Dehalospirillum multivorans

Taxonomic identifier

66821 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Sulfurospirillum

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP cyclohydrolase II activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_0000151796
Regions
	Region	1 – 211	211	DHBP synthase HAMAP MF_00180
	Region	38 – 39	2	Substrate binding By similarity
	Region	150 – 154	5	Substrate binding By similarity
	Region	212 – 356	145	GTP cyclohydrolase II-like HAMAP MF_00180
Sites
	Metal binding	39	1	Magnesium or manganese 1 By similarity
	Metal binding	39	1	Magnesium or manganese 2 By similarity
	Metal binding	153	1	Magnesium or manganese 2 By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Site	136	1	Essential for catalytic activity By similarity
	Site	174	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O68249-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 851685138C2F6512	FASTA	356	39,358
	10 20 30 40 50 60 MNAILSDQKT FQAIIRVNQA IEDIRQGKMV VMVDDEDREN EGDLVYAASF STPQKVNFMA 70 80 90 100 110 120 SHAKGLICVA ISKKIANRLQ LEPMVKKNDS SYETAFTITV DARTAATGIS AGERDMTIKI 130 140 150 160 170 180 LADGGSHESE LVRPGHIFPL IAKEGGALVR IGHTEGSVDL CRLAGQGDSA VICEIMKEDG 190 200 210 220 230 240 TMARRPDLDI FCAKHELNIV YISDIVEYRM MNESLIRVIA ESTTQFLGKD ARRYDFVDHN 250 260 270 280 290 300 DNHHIAYAFG NIKNRSAVKF HSIMPDNELL ADTKKYNSLI QAIHYLQKSG GVLIFMDNGT 310 320 330 340 350 QDMSKIREYG IGAQIIKHLG IENIELLSDS KNKEFVGISG FGLSVIKSTN VNETVA

References

[1]

"Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli." Neumann A., Wohlfarth G., Diekert G. J. Bacteriol. 180:4140-4145(1998) [PubMed: 9696761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022812 Genomic DNA. Translation: AAC60785.1.
3D structure databases
HSSP	HSSP built from PDB template 1G58 based on UniProtKB P24199.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.99.12. 229899.
Family and domain databases
HAMAP	MF_00180. Fused. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
ProDom	PD003034. DHBP_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_DEHMU

Accession

Primary (citable) accession number: O68249

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	September 22, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)