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O68186 (PUR5_LACLM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine cyclo-ligase

EC=6.3.3.1
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:purM
Ordered Locus Names:llmg_0987
OrganismLactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]
Taxonomic identifier416870 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00741.

Sequence similarities

Belongs to the AIR synthase family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine cyclo-ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741
PRO_0000148217

Sequences

Sequence LengthMass (Da)Tools
O68186 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E220C4C8780CBBE0

FASTA34036,292
        10         20         30         40         50         60 
MTVSENAYAK SGVDVEAGYE VVSRIKKHVA KTERLGVLGA LGGFGGSFDL SVLDVKEPVL 

        70         80         90        100        110        120 
ISGTDGVGTK LMLAIRADKH DTIGIDCVAM CVNDIIAAGA EPLYFLDYIA TGKNIPEKLE 

       130        140        150        160        170        180 
QVVAGVAEGC LQAGAALIGG ETAEMPGMYD EDDYDLAGFA VGVAEKSQLI DGEKDVEAGD 

       190        200        210        220        230        240 
VLLGLASSGI HSNGYSLVRK VFADFDLNES LPELDQSLID TLLTPTKIYV KELLPLIKQN 

       250        260        270        280        290        300 
KIKGIAHITG GGFHENLPRM FGNSLSAEIV EGSWDVLPIF KALEKYGSIK HEEMYEIFNM 

       310        320        330        340 
GIGMVIAVAP ENAAALKKEL NAFEIGQMVN RQEAPVVIKK 

« Hide

References

« Hide 'large scale' references
[1]"Chaperones and ATP-dependent proteases of Lactococcus lactis."
Coward C.
Thesis (1998), University of Cambridge, United Kingdom
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MG1363.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF016634 Genomic DNA. Translation: AAC16901.1.
AM406671 Genomic DNA. Translation: CAL97580.1.
RefSeqYP_001032308.1. NC_009004.1.

3D structure databases

ProteinModelPortalO68186.
SMRO68186. Positions 16-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416870.llmg_0987.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL97580; CAL97580; llmg_0987.
GeneID4797128.
KEGGllm:llmg_0987.
PATRIC22283100. VBILacLac4574_1008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0150.
HOGENOMHOG000229090.
KOK01933.
OMAHCVNDIL.
OrthoDBEOG61CM1V.
ProtClustDBPRK05385.

Enzyme and pathway databases

BioCycLLAC416870:GCDT-1012-MONOMER.
UniPathwayUPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPMF_00741_B. AIRS_B.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsTIGR00878. purM. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR5_LACLM
AccessionPrimary (citable) accession number: O68186
Secondary accession number(s): A2RJX4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways