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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi208 – 2158ATP 1By similarity
Nucleotide bindingi608 – 6158ATP 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-1011-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:llmg_0986
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 867867Chaperone protein ClpBPRO_0000005493Add
BLAST

Proteomic databases

PRIDEiO68185.

Expressioni

Inductioni

By heat shock.1 Publication

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi416870.llmg_0986.

Structurei

3D structure databases

ProteinModelPortaliO68185.
SMRiO68185. Positions 161-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 145145N-terminalBy similarityAdd
BLAST
Regioni161 – 342182NBD1By similarityAdd
BLAST
Regioni343 – 548206LinkerBy similarityAdd
BLAST
Regioni558 – 770213NBD2By similarityAdd
BLAST
Regioni771 – 86797C-terminalBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili393 – 527135By similarityAdd
BLAST

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218211.
KOiK03695.
OMAiQQEKGAI.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform ClpB (identifier: O68185-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIEKMTTTM QEALGSAQQI AQVRHHQVIE VPHLWRIFVQ PNSFGANFYK
60 70 80 90 100
DLGIDLDDFT NLIEKEIDKI NSVEGSNITY GQNLSPDLFQ VFTEADKIAQ
110 120 130 140 150
KMGDEYLSTE IILLALFELK QNPLTEYLVS HGLTKAKAQA AIEKLRGGDK
160 170 180 190 200
VTSQNAEETY KALEKYGVDL VAQVKSGNQD PVIGRDEEIR DVIRVLSRKT
210 220 230 240 250
KNNPVLIGEP GVGKTAIVEG LAQRIVRKDV PENLKDKTIF SLDMGALIAG
260 270 280 290 300
AKYRGEFEER LKAVLNEVKK ADGQIILFID ELHTIVGAGK TEGSMDAGNL
310 320 330 340 350
LKPMLARGEL HLIGATTLDE YRKYMETDKA LERRFQKVLV TEPTVEDTIS
360 370 380 390 400
ILRGLKERFE IHHGVTIHDN ALVAAATLSN RYITDRFLPD KAIDLIDEAS
410 420 430 440 450
ATIRVEMNSL PTELDQANRR LMQLEIEEAA LKKERDDASK KRLEIIRGEI
460 470 480 490 500
AELREENNQL KAQWEAEKKE VGNISEKRNE LEHARHELEE AQNEGNLEKA
510 520 530 540 550
AALRYGKIPE IEKELKAIEE KAKSDDLSLV QESVTEEQIA EVVGRMTGIP
560 570 580 590 600
ITKLVEGERE KLLHLPETLH QRVVGQDEAV EAVSDAIIRA RAGIQDPNRP
610 620 630 640 650
LGSFLFLGPT GVGKTELAKA LAENLFDSEE HMVRIDMSEY MEKHSVSRLV
660 670 680 690 700
GAPPGYVGYD EGGQLTEAVR RNPYTIILLD EIEKAHPDVF NILLQVLDDG
710 720 730 740 750
RLTDSKGVLV DFKNTVLIMT SNVGSQYLLD NVGENGEISE ETTENVMSQL
760 770 780 790 800
RAHFKPEFLN RIDDTILFKP LALEDIKNII LKMTSQLAHR LEEMEVELEL
810 820 830 840 850
SEEVKVWIAE NAYEPAYGAR PLKRYLTKVI ENPLAKLIIG GKIPPKSKVI
860
VRLIDNKVDF DVQSIAE
Length:867
Mass (Da):97,327
Last modified:May 1, 2007 - v3
Checksum:iC2A3E907F0BAC573
GO
Isoform ClpB' (identifier: O68185-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.
     151-151: V → M

Show »
Length:717
Mass (Da):80,451
Checksum:i5B911B803B55403C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti358 – 3581R → C in AAD01784 (PubMed:10094684).Curated
Sequence conflicti373 – 3731V → I in AAC16900 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 150150Missing in isoform ClpB'. CuratedVSP_018704Add
BLAST
Alternative sequencei151 – 1511V → M in isoform ClpB'. CuratedVSP_018705

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016634 Genomic DNA. Translation: AAC16900.1.
AM406671 Genomic DNA. Translation: CAL97579.1.
AF023423 Genomic DNA. Translation: AAD01784.1.
RefSeqiWP_011834922.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL97579; CAL97579; llmg_0986.
KEGGillm:llmg_0986.
PATRICi22283098. VBILacLac4574_1007.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016634 Genomic DNA. Translation: AAC16900.1.
AM406671 Genomic DNA. Translation: CAL97579.1.
AF023423 Genomic DNA. Translation: AAD01784.1.
RefSeqiWP_011834922.1. NC_009004.1.

3D structure databases

ProteinModelPortaliO68185.
SMRiO68185. Positions 161-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_0986.

Proteomic databases

PRIDEiO68185.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL97579; CAL97579; llmg_0986.
KEGGillm:llmg_0986.
PATRICi22283098. VBILacLac4574_1007.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218211.
KOiK03695.
OMAiQQEKGAI.

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-1011-MONOMER.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_LACLM
AccessioniPrimary (citable) accession number: O68185
Secondary accession number(s): A2RJX3, Q9S5Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.