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O68141

- TRMFO_RHOCB

UniProt

O68141 - TRMFO_RHOCB

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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 136FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciRCAP272942:GJIY-1991-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:RCAP_rcc01962
OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Taxonomic identifieri272942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002361: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117256Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO68141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiWEMRAAG.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68141-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDRLHIIGAG LAGSEAAWQA AQAGVPVVLH EMRPRVGTFA HRSGDFAEMV
60 70 80 90 100
CSNSFRSDDD ERNAVGLLHW EMRAAGGLIM ATADRHALPA GGALAVDREA
110 120 130 140 150
FSGAVTAALR AHPLISVAEE EITELPAEGH WIIATGPLTS GALAESIRAV
160 170 180 190 200
TGAESLAFFD AIAPIVHAET TDIRCLRQSR YDKGETEEER TAYINCPMTR
210 220 230 240 250
DQYEAFIDAL LAAEKTEFHA GETAGYFDGC LPIEVMAERG RETLRHGPMK
260 270 280 290 300
PVGLTNAHDP ATKAYAVVQL RRDNALGTLY NIVGFQTKMK YGAQTAVFKM
310 320 330 340 350
IPGLEQASFA RLGGIHRNTF LNSPTLLDDQ MRLRARPNLR FAGQVTGVEG
360 370 380 390 400
YVESAAMGLL AGRMAAAGIL GRSLPPPGPE TAMGALVNHI TGGAVAKTFQ
410 420 430 440
PMNVNFGLFP PLDDARGGRR GRKDRYKGYT DRAKEVFTAW LAAQA
Length:445
Mass (Da):47,855
Last modified:May 31, 2011 - v2
Checksum:iEEBB2AA207113AB7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 21811DALLAAEKTEF → GRAARGREDRV in AAC16231. (PubMed:9256491)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010496 Genomic DNA. Translation: AAC16231.1.
CP001312 Genomic DNA. Translation: ADE85706.1.
PIRiT03578.
RefSeqiWP_013067685.1. NC_014034.1.
YP_003578113.1. NC_014034.1.

Genome annotation databases

EnsemblBacteriaiADE85706; ADE85706; RCAP_rcc01962.
GeneIDi9004785.
KEGGircp:RCAP_rcc01962.
PATRICi35504086. VBIRhoCap134200_1997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010496 Genomic DNA. Translation: AAC16231.1 .
CP001312 Genomic DNA. Translation: ADE85706.1 .
PIRi T03578.
RefSeqi WP_013067685.1. NC_014034.1.
YP_003578113.1. NC_014034.1.

3D structure databases

ProteinModelPortali O68141.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE85706 ; ADE85706 ; RCAP_rcc01962 .
GeneIDi 9004785.
KEGGi rcp:RCAP_rcc01962.
PATRICi 35504086. VBIRhoCap134200_1997.

Phylogenomic databases

HOGENOMi HOG000252054.
KOi K04094.
OMAi WEMRAAG.

Enzyme and pathway databases

BioCyci RCAP272942:GJIY-1991-MONOMER.

Family and domain databases

HAMAPi MF_01037. TrmFO.
InterProi IPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view ]
Pfami PF01134. GIDA. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00137. gid_trmFO. 1 hit.
PROSITEi PS01281. GIDA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003."
    Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.
    Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
  2. "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
    Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
    J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

Entry informationi

Entry nameiTRMFO_RHOCB
AccessioniPrimary (citable) accession number: O68141
Secondary accession number(s): D5AUR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 31, 2011
Last modified: November 26, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3