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O68141

- TRMFO_RHOCB

UniProt

O68141 - TRMFO_RHOCB

Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

    Cofactori

    FAD.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 136FADUniRule annotation

    GO - Molecular functioni

    1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
    2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
    3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciRCAP272942:GJIY-1991-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
    Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
    Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmFOUniRule annotation
    Synonyms:gid
    Ordered Locus Names:RCAP_rcc01962
    OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
    Taxonomic identifieri272942 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000002361: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117256Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO68141.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000252054.
    KOiK04094.
    OMAiWEMRAAG.

    Family and domain databases

    HAMAPiMF_01037. TrmFO.
    InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view]
    PfamiPF01134. GIDA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
    PROSITEiPS01281. GIDA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O68141-1 [UniParc]FASTAAdd to Basket

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    MDRLHIIGAG LAGSEAAWQA AQAGVPVVLH EMRPRVGTFA HRSGDFAEMV    50
    CSNSFRSDDD ERNAVGLLHW EMRAAGGLIM ATADRHALPA GGALAVDREA 100
    FSGAVTAALR AHPLISVAEE EITELPAEGH WIIATGPLTS GALAESIRAV 150
    TGAESLAFFD AIAPIVHAET TDIRCLRQSR YDKGETEEER TAYINCPMTR 200
    DQYEAFIDAL LAAEKTEFHA GETAGYFDGC LPIEVMAERG RETLRHGPMK 250
    PVGLTNAHDP ATKAYAVVQL RRDNALGTLY NIVGFQTKMK YGAQTAVFKM 300
    IPGLEQASFA RLGGIHRNTF LNSPTLLDDQ MRLRARPNLR FAGQVTGVEG 350
    YVESAAMGLL AGRMAAAGIL GRSLPPPGPE TAMGALVNHI TGGAVAKTFQ 400
    PMNVNFGLFP PLDDARGGRR GRKDRYKGYT DRAKEVFTAW LAAQA 445
    Length:445
    Mass (Da):47,855
    Last modified:May 31, 2011 - v2
    Checksum:iEEBB2AA207113AB7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti208 – 21811DALLAAEKTEF → GRAARGREDRV in AAC16231. (PubMed:9256491)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010496 Genomic DNA. Translation: AAC16231.1.
    CP001312 Genomic DNA. Translation: ADE85706.1.
    PIRiT03578.
    RefSeqiYP_003578113.1. NC_014034.1.

    Genome annotation databases

    EnsemblBacteriaiADE85706; ADE85706; RCAP_rcc01962.
    GeneIDi9004785.
    KEGGircp:RCAP_rcc01962.
    PATRICi35504086. VBIRhoCap134200_1997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010496 Genomic DNA. Translation: AAC16231.1 .
    CP001312 Genomic DNA. Translation: ADE85706.1 .
    PIRi T03578.
    RefSeqi YP_003578113.1. NC_014034.1.

    3D structure databases

    ProteinModelPortali O68141.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE85706 ; ADE85706 ; RCAP_rcc01962 .
    GeneIDi 9004785.
    KEGGi rcp:RCAP_rcc01962.
    PATRICi 35504086. VBIRhoCap134200_1997.

    Phylogenomic databases

    HOGENOMi HOG000252054.
    KOi K04094.
    OMAi WEMRAAG.

    Enzyme and pathway databases

    BioCyci RCAP272942:GJIY-1991-MONOMER.

    Family and domain databases

    HAMAPi MF_01037. TrmFO.
    InterProi IPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view ]
    Pfami PF01134. GIDA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00137. gid_trmFO. 1 hit.
    PROSITEi PS01281. GIDA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003."
      Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.
      Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
    2. "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
      Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
      J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

    Entry informationi

    Entry nameiTRMFO_RHOCB
    AccessioniPrimary (citable) accession number: O68141
    Secondary accession number(s): D5AUR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3