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O68141 (TRMFO_RHOCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:RCAP_rcc01962
OrganismRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) [Complete proteome] [HAMAP]
Taxonomic identifier272942 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000117256

Regions

Nucleotide binding8 – 136FAD By similarity

Experimental info

Sequence conflict208 – 21811DALLAAEKTEF → GRAARGREDRV in AAC16231. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O68141 [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: EEBB2AA207113AB7

FASTA44547,855
        10         20         30         40         50         60 
MDRLHIIGAG LAGSEAAWQA AQAGVPVVLH EMRPRVGTFA HRSGDFAEMV CSNSFRSDDD 

        70         80         90        100        110        120 
ERNAVGLLHW EMRAAGGLIM ATADRHALPA GGALAVDREA FSGAVTAALR AHPLISVAEE 

       130        140        150        160        170        180 
EITELPAEGH WIIATGPLTS GALAESIRAV TGAESLAFFD AIAPIVHAET TDIRCLRQSR 

       190        200        210        220        230        240 
YDKGETEEER TAYINCPMTR DQYEAFIDAL LAAEKTEFHA GETAGYFDGC LPIEVMAERG 

       250        260        270        280        290        300 
RETLRHGPMK PVGLTNAHDP ATKAYAVVQL RRDNALGTLY NIVGFQTKMK YGAQTAVFKM 

       310        320        330        340        350        360 
IPGLEQASFA RLGGIHRNTF LNSPTLLDDQ MRLRARPNLR FAGQVTGVEG YVESAAMGLL 

       370        380        390        400        410        420 
AGRMAAAGIL GRSLPPPGPE TAMGALVNHI TGGAVAKTFQ PMNVNFGLFP PLDDARGGRR 

       430        440 
GRKDRYKGYT DRAKEVFTAW LAAQA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003."
Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.
Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[2]"Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF010496 Genomic DNA. Translation: AAC16231.1.
CP001312 Genomic DNA. Translation: ADE85706.1.
PIRT03578.
RefSeqYP_003578113.1. NC_014034.1.

3D structure databases

ProteinModelPortalO68141.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE85706; ADE85706; RCAP_rcc01962.
GeneID9004785.
KEGGrcp:RCAP_rcc01962.
PATRIC35504086. VBIRhoCap134200_1997.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000252054.
KOK04094.
OMAWEMRAAG.

Enzyme and pathway databases

BioCycRCAP272942:GJIY-1991-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_RHOCB
AccessionPrimary (citable) accession number: O68141
Secondary accession number(s): D5AUR7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 31, 2011
Last modified: May 14, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families