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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATPUniRule annotation
Binding sitei54 – 541ATPUniRule annotation
Binding sitei81 – 811ATPUniRule annotation
Binding sitei353 – 3531ATPUniRule annotation
Metal bindingi439 – 4391Magnesium 1; catalyticUniRule annotation
Metal bindingi512 – 5121Magnesium 1; catalyticUniRule annotation
Metal bindingi512 – 5121Magnesium 2UniRule annotation
Metal bindingi514 – 5141Magnesium 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1297ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)Imported
Taxonomic identifieri1061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei464 – 4641Interaction with DNAUniRule annotation
Sitei467 – 4671Interaction with DNAUniRule annotation
Sitei519 – 5191Interaction with DNAUniRule annotation
Sitei636 – 6361Interaction with DNAUniRule annotation

Protein-protein interaction databases

STRINGi272942.RCAP_rcc02079.

Structurei

3D structure databases

ProteinModelPortaliO68071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini433 – 547115ToprimUniRule annotationInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
HOGENOMiHOG000075154.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O68071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDLLSSTP SETYDASSIE VLEGLEPVRK RPGMYIGGTD ERALHHMVAE
60 70 80 90 100
ILDNAMDEAV AGHANRIEVE VNADGSVTVR DNGRGIPVDP HPKFPGKSAL
110 120 130 140 150
EVILCTLHAG GKFSNKAYST SGGLNGVGSS VVNALSDLMV VQVARNKELY
160 170 180 190 200
EQRFSRGVPQ GPVAKLGAAP NRRGTTVTFH PDPEIFGSLS LKPARLFKMT
210 220 230 240 250
RSKAYLFSGV EIRWKTAIAD GDTPTEATFH FPNGLADYLA EALTGASTYS
260 270 280 290 300
DKPFAGRVSF EEKYNIPGSV EWAINWTPVR DGFIHSYCNT VPTPEGGTHE
310 320 330 340 350
AGFWAAILKG IRAYGERINN KKAAQITRED MLTGGCALIS IFIREPSFVG
360 370 380 390 400
QTKDRLSTEE AARWVENAVR DYFDTWLTTD TKAAGAILDF LVLRAEERLR
410 420 430 440 450
RKAEKETSRK SATKKLRLPG KLVDCSATNR EGTELFIVEG DSAGGSAKMA
460 470 480 490 500
RDRTMQALLP LRGKILNVLG AASSKLGQNQ EINDLAQALG VGLGTKFRVD
510 520 530 540 550
DLRYDKIIIM TDADVDGAHI ASLLMTFFFT QMRGLIDKGH LYLACPPLYR
560 570 580 590 600
LTQGAKRIYV ADDAEKDVWM AKGLGGRGKI DVQRFKGLGE MDAKDLKDTT
610 620 630 640 650
MNPATRKLIR VTIDDEFAGE TDDLVERLMG KKPELRFQYI TENARFVEEL

DV
Length:652
Mass (Da):71,524
Last modified:August 1, 1998 - v1
Checksum:i4CE2687B47F4289D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010496 Genomic DNA. Translation: AAC16157.1.
PIRiT03504.

Genome annotation databases

PATRICi35504328. VBIRhoCap134200_2116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010496 Genomic DNA. Translation: AAC16157.1.
PIRiT03504.

3D structure databases

ProteinModelPortaliO68071.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272942.RCAP_rcc02079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi35504328. VBIRhoCap134200_2116.

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
HOGENOMiHOG000075154.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003."
    Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.
    Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: SB1003Imported.

Entry informationi

Entry nameiO68071_RHOCA
AccessioniPrimary (citable) accession number: O68071
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: March 16, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.