O68008 (BACC_BACLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 83.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bacitracin synthase 3 Short name=BA3 Including the following 7 domains:
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| Gene names |
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| Organism | Bacillus licheniformis | ||
| Taxonomic identifier | 1402 [NCBI] | ||
| Taxonomic lineage | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›  |
Protein attributes
| Sequence length | 6359 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Induces peptide synthesis, activates and incorporates five amino acids, forms a thiazoline ring between the first two amino acids and incoporates a D-glutamine in the fourth position. |
| Catalytic activity | L-aspartate = D-aspartate. ATP + L-phenylalanine + H2O = AMP + diphosphate + D-phenylalanine. |
| Cofactor | Binds 5 phosphopantetheines covalently Potential. |
| Pathway | |
| Subunit structure | Large multienzyme complex of BA1, BA2 and BA3. |
| Domain | Consists of five modules with two epimerization domains in the second and fourth modules, and a putative C-terminal thioesterase domain. Each module incorporates one amino acid into the peptide product and can be further subdivided into domains responsible for substrate adenylation, thiolation, condensation (not for the initiation module), and epimerization (optional), and N methylation (optional). |
| Miscellaneous | Bacitracin is a mixture of at least ten cyclic dodecapeptides, that differ by one or two amino acids. The most abundant is bacitracin A, a branched cyclic dodecapeptide. It contains an N-terminal linear pentapeptide moiety (Ile-Cys-Leu-D-Glu-Ile) with an isoleucine-cysteine thiazoline condensation product and a C-terminal heptapeptide ring (Lys-D-Orn-Ile-D-Phe-His-D-Asp-Asn), in which the free alpha-carboxy group of the C-terminal Asn is bound to the epsilon-amino group of Lys. |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. Contains 5 acyl carrier domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antibiotic biosynthesis |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding Phosphopantetheine |
| Molecular function | Hydrolase Isomerase Ligase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological_process | antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate racemase activityInferred from electronic annotation. Source: EC hydrolase activity, acting on ester bondsInferred from electronic annotation. Source: InterPro ligase activityInferred from electronic annotation. Source: UniProtKB-KW phenylalanine racemase (ATP-hydrolyzing) activityInferred from electronic annotation. Source: EC phosphopantetheine bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 6359 | 6359 | Bacitracin synthase 3 | PRO_0000193084 | |||||
Regions | |||||||||
| Domain | 966 – 1033 | 68 | Acyl carrier 1 | ||||||
| Domain | 1998 – 2064 | 67 | Acyl carrier 2 | ||||||
| Domain | 3502 – 3569 | 68 | Acyl carrier 3 | ||||||
| Domain | 4544 – 4610 | 67 | Acyl carrier 4 | ||||||
| Domain | 6052 – 6119 | 68 | Acyl carrier 5 | ||||||
| Region | 461 – 1034 | 574 | Domain 1 (isoleucine-activating) | ||||||
| Region | 1517 – 2064 | 548 | Domain 2 (D-phenylalanine-activating) | ||||||
| Region | 2999 – 3570 | 572 | Domain 3 (histidine-activating) | ||||||
| Region | 4047 – 4612 | 566 | Domain 4 (D-aspartic acid-activating) | ||||||
| Region | 5549 – 6129 | 581 | Domain 5 (asparagine-activating) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 996 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
| Modified residue | 2028 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
| Modified residue | 3532 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
| Modified residue | 4574 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
| Modified residue | 6082 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
Sequences
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References
| [1] | "The bacitracin biosynthesis operon of Bacillus licheniformis ATCC 10716: molecular characterization of three multi-modular peptide synthetases." Konz D., Klens A., Schoergendorfer K., Marahiel M.A. Chem. Biol. 4:927-937(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10716 / DSM 603 / NBRC 12199 / NCIMB 8874. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF007865 Genomic DNA. Translation: AAC06348.1. |
| PIR | T31679. |
3D structure databases | |
| ProteinModelPortal | O68008. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Enzyme and pathway databases | |
| UniPathway | UPA00179. |
Family and domain databases | |
| Gene3D | 1.10.1200.10. 5 hits. |
| InterPro | IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR001242. Condensatn. IPR010060. NRPS_synth. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR001031. Thioesterase. [Graphical view] |
| Pfam | PF00501. AMP-binding. 5 hits. PF00668. Condensation. 7 hits. PF00550. PP-binding. 5 hits. PF00975. Thioesterase. 1 hit. [Graphical view] |
| SMART | SM00823. PKS_PP. 5 hits. [Graphical view] |
| SUPFAM | SSF47336. ACP_like. 5 hits. |
| TIGRFAMs | TIGR01733. AA-adenyl-dom. 5 hits. TIGR01720. NRPS-para261. 2 hits. |
| PROSITE | PS50075. ACP_DOMAIN. 5 hits. PS00455. AMP_BINDING. 5 hits. PS00012. PHOSPHOPANTETHEINE. 4 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BACC_BACLI | ||||||||
| Accession | Primary (citable) accession number: O68008 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |