O68006 (BACA_BACLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bacitracin synthase 1
Short name=BA1

Including the following 6 domains:

1. ATP-dependent isoleucine adenylase
   Short name=IleA
   Alternative name(s):
   Isoleucine activase
2. ATP-dependent cysteine adenylase
   Short name=CysA
   Alternative name(s):
   Cysteine activase
3. ATP-dependent leucine adenylase
   Short name=LeuA
   Alternative name(s):
   Leucine activase
4. ATP-dependent glutamate adenylase
   Short name=GluA
   Alternative name(s):
   Glutamate activase
5. ATP-dependent isoleucine adenylase
   Short name=IleA
   Alternative name(s):
   Isoleucine activase
6. Glutamate racemase
   EC=5.1.1.3

Gene names

Name:

bacA

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	5255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin.
Catalytic activity	L-glutamate = D-glutamate.
Cofactor	Binds 5 phosphopantetheines covalently Potential.
Pathway	Antibiotic biosynthesis; bacitracin biosynthesis.
Subunit structure	Large multienzyme complex of BA1, BA2 and BA3.
Domain	Consists of five modules and one epimerization domain in the fourth module. Each module incorporates one amino acid into the peptide product and can be further subdivided into domains responsible for substrate adenylation, thiolation, condensation (not for the initiation module), and epimerization (optional), and N methylation (optional).
Miscellaneous	Bacitracin is a mixture of at least ten cyclic dodecapeptides, that differ by one or two amino acids. The most abundant is bacitracin A, a branched cyclic dodecapeptide. It contains an N-terminal linear pentapeptide moiety (Ile-Cys-Leu-D-Glu-Ile) with an isoleucine-cysteine thiazoline condensation product and a C-terminal heptapeptide ring (Lys-D-Orn-Ile-D-Phe-His-D-Asp-Asn), in which the free alpha-carboxy group of the C-terminal Asn is bound to the epsilon-amino group of Lys.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Contains 5 acyl carrier domains.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Domain	Repeat
Ligand	Phosphopantetheine
Molecular function	Isomerase Ligase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro glutamate racemase activity Inferred from electronic annotation. Source: EC ligase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 5255	5255	Bacitracin synthase 1		PRO_0000193082
Regions
	Domain	544 – 611	68	Acyl carrier 1
	Domain	1585 – 1652	68	Acyl carrier 2
	Domain	2621 – 2688	68	Acyl carrier 3
	Domain	3664 – 3730	67	Acyl carrier 4
	Domain	5171 – 5238	68	Acyl carrier 5
	Region	39 – 612	574	Domain 1 (isoleucine-activating)
	Region	621 – 1037	417	Cyclization Potential
	Region	1109 – 1648	540	Domain 2 (cysteine-activating)
	Region	2124 – 2689	566	Domain 3 (leucine-activating)
	Region	3164 – 3732	569	Domain 4 (glutamine-activating)
	Region	4668 – 5249	582	Domain 5 (isoleucine-activating)
Amino acid modifications
	Modified residue	574	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	1615	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	2651	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	3694	1	O-(pantetheine 4'-phosphoryl)serine By similarity
	Modified residue	5201	1	O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O68006 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 906E8DD68450F85B	FASTA	5,255	598,269
	10 20 30 40 50 60 MVAKHSLENG VFHKMTENEK ELILHFNNTK TDYPKNKTLH ELFEEQAMKT PDHTALVFGA 70 80 90 100 110 120 QRMTYRELNE KANQTARLLR EKGIGRGSIA AIIADRSFEM IIGIIGILKA GGAYLPIDPE 130 140 150 160 170 180 TPKDRIAFML SDTKAAVLLT QGKAADGIDC EADIVQLDRE ASDGFSKEPL SSVNDSGDTA 190 200 210 220 230 240 YIIYTSGSTG TPKGVITPHY SVIRVVQNTN YIDITEDNVI LQLSNYSFDG SVFDIFGALL 250 260 270 280 290 300 NGASLVMIEK EALLNINRLG SAINEEKVSV MFITTALFNM IADIHVDCLS NLRKILFGGE 310 320 330 340 350 360 RASIPHVRKV LNHVGRDKLI HVYGPTESTV YATYYFINEI DDEAETIPIG SPLANTSVLI 370 380 390 400 410 420 MDEAGKLVPI GVPGELCIAG DGLSKGYLNR EELTAEKFIP HPFIPGERLY KTGDLAKWLP 430 440 450 460 470 480 DGNIEFIGRI DHQVKIRGFR IELGEIESRL EMHEDINETI VTVREDEESR PYICAYITAN 490 500 510 520 530 540 REISLDELKG FLGEKLPEYM IPAYFVKLDK LPLTKNGKVD RKALPEPDRT AGAENEYEAP 550 560 570 580 590 600 RNETEEKLAA VWQDVLHVEK AGIHDHFAQM GGHSLHAMEL IAKIKEKMNV EIPLHQLFKL 610 620 630 640 650 660 ATIKELSAFI EANHQEDKGD TLVTRAADPE NIHEIFPLTG IQLAYLVGRD ETFEIGGVAT 670 680 690 700 710 720 NLTVEFEADV DLNRFQLTLQ KLIDRHPILR TIVFENGTQK ILEATQRYTI ETQDLRGFTE 730 740 750 760 770 780 EEINVRILEQ REKMTSKIID PSVWPLFELK TFMLPGEKKY FFLNVDPLIC DDSSMKRLIR 790 800 810 820 830 840 EFKQLYENPG LQLPSLEYSF RDYVLASINF KQTSRYQKDQ QYWLDKLDHF PSAPELPLKS 850 860 870 880 890 900 DPAHVAKPSF KKFSTFLDGH TWNELKKKAR HHHLTPTSVL CAAYAYILAY WSRQNHFAIN 910 920 930 940 950 960 LTVFNRIPFH PDVKNMIGDF TSLMLLDIHA EENMSSFWRF ALNVQDTLLE ALEHRHYDGV 970 980 990 1000 1010 1020 DVIRNIAKKN GMNKKAVMPI VFTSVLSENP DDSFDSLVDF DNIHFFSTRT SQVYIDNQVY 1030 1040 1050 1060 1070 1080 EINGGLYITW DYVEQIFEHE VIESMFDQYI AVIQKAVSGE DVSTIQMNEK SRQMISAYND 1090 1100 1110 1120 1130 1140 TDQSFDAKPL HELFTGQVKH GPDRMALKHH DEVMTYQELD EKSNQVARFL IGKGVEKGDY 1150 1160 1170 1180 1190 1200 IGVIGKRSLG TIVNLLAVLK TGAAYIPLDP DYPEERKAYI QSKSNCKFFI SHDVYDKEHI 1210 1220 1230 1240 1250 1260 ERFSKAPVDR KVDLDDMAYV IFTSGSTGKP KGVQITHVPQ RNTILDINEK FNVTEQDNIM 1270 1280 1290 1300 1310 1320 GISSLCFDLS VYDVFGALSS GASLVIIDDQ RDVFSLKETA EKERITIWNS VPAIMGMTAD 1330 1340 1350 1360 1370 1380 VYPDNELNHH LRLILLSGDW IPLQLPATIK KTFKNAEVIS LGGATEGSIW SIYYPIQKVE 1390 1400 1410 1420 1430 1440 EDWKSIPYGK PLANQKIYVL NQNKQLCPVG VEGELYIGGA GVASGYIHDQ EKTEHSFIQH 1450 1460 1470 1480 1490 1500 QELGYIYKTG DYGVLKEDGY VEFLGRKDSQ VKIRGYRVEM GEIENTLVSH QEITKASVID 1510 1520 1530 1540 1550 1560 YTSPDGIKNL YAFVVAENAI SQLDVKEFLQ KTLPDYMIPA KFVQIEEIPL TVNGKVDKRT 1570 1580 1590 1600 1610 1620 LHDLAEQHTA DEGQRGGRML PENETQAMLL EIWKDIFGLD SINLDVSYYE IGGDSLKAIS 1630 1640 1650 1660 1670 1680 IITEINKRMN VEMPISEIFK NDTIIALDHY LKNREESDME HPIQKAREKE YYPTSPAQQR 1690 1700 1710 1720 1730 1740 MYMLSMLENE RGAYHIPMAL LVEGRINAMQ LENALKTFLQ RHEILRTGFE IQNNELIQKI 1750 1760 1770 1780 1790 1800 YENVDFRLEY ECLDASITDQ HALMEITSRY CKESIKPFDL SRPPLMRAKL IKIDDIRHIL 1810 1820 1830 1840 1850 1860 VINFHHIISD GVSQGILMNE ILELYSNVPL PEVNVQYKDY VEWNHTFNQS AAMKKQEAYW 1870 1880 1890 1900 1910 1920 LDVYRDIPSK LDFPYDYKRH HIDTFEGSSV FLEMERELSD HIRKLAKHNG TTLYTVMLSA 1930 1940 1950 1960 1970 1980 YYVLLNKYTN QTDIVVGTAA AGRLHPDLQD VFGVFVNTLA LRNEVDTSYS FKEFLQQTKE 1990 2000 2010 2020 2030 2040 RTIAAFDNSE YPFDDLIRKL NGVRESNRNP LFDTMFVLED ARMFTKQKGD VKLSPIIFEL 2050 2060 2070 2080 2090 2100 DNAKFDMIFN VLDFEQKIVL NIEYSTSLFK DETIQKIAED YFRILEEVSE NLDVALHQID 2110 2120 2130 2140 2150 2160 MISRQEKRTL LESFNHTKTA YPKGKAIHQL FEEQAKRIPD HTAVVFEDQK LTYRQLNEKA 2170 2180 2190 2200 2210 2220 NQVARLLREK GVKPDTLVGI MMERSSDMIA AILGVLKAGG AYLPIDPEYP PERMRYMAFD 2230 2240 2250 2260 2270 2280 SEVKVIISDV PLAEELTAES IELIHMDDER IAGQDRSDID NVNQSGDLAY VIYTSGSTGK 2290 2300 2310 2320 2330 2340 PKGVMIEHQS LINLCSWHQS CFEVGQNDNS SIYASISFDA FVWELFPYIT AGATVHVLNQ 2350 2360 2370 2380 2390 2400 ETRLDVEKLN RYFHDHHITI SFLPTPVCEQ FTALDNHSLR TLLTGGDKLN VFKEKSYQIV 2410 2420 2430 2440 2450 2460 NNYGPTENTV VATSFPIDKS HQNIPIGKPI DNVKVYILNK DLQLCPLGAS GELCIAGEGL 2470 2480 2490 2500 2510 2520 ARGYVNRPEL TREKFIGNPF VPGERMYRTG DLAKMLPDGN IQFLGRVDQQ VKIRGYRIEP 2530 2540 2550 2560 2570 2580 GEIENRLLKY EKIEEAAVIA REDGDHDPYL CAYVTVKKEV EPEKIRAFLK KSLPDYMIPQ 2590 2600 2610 2620 2630 2640 YFVQLDGLPL TVNGKVDKKS LPVPERSVTM DRRYEAPRDQ MEEKLVSIWE EALGINKIGI 2650 2660 2670 2680 2690 2700 NSHFFEAGGH SLKAAALVST IHKELNVKLP LRQIFETPTI KGLRDISVRR RKCFYIDRKT 2710 2720 2730 2740 2750 2760 EEKPYYRLSS AQKRLYILSQ TGSHVAYNMP FAMTLEGDFD IRRFENTLKN MVKRHESFRT 2770 2780 2790 2800 2810 2820 SFVMIDGEVM QQIEKEIDFQ VAYSDIGKES AEEKIKSFIR PFHLEKAPLL RAEVVKLNER 2830 2840 2850 2860 2870 2880 EHLLMFDMHH IISDGVSTDI FIQELGALYE GKSLKPFHIQ YKDYAEWENS HARSEELKRQ 2890 2900 2910 2920 2930 2940 EEYWLKTYKG DIPVLDLPID HKRPLTKSSE GDTVTAAIES ETFRKLQHMA KENGVTMYML 2950 2960 2970 2980 2990 3000 LLAGYTALLS KYTGQEDIIV GTPAAGRNHE DIQHLIGMFV NTLAIRNHPE GKKTFRDYLQ 3010 3020 3030 3040 3050 3060 EVKENTLQAY ENQDYPFEEL VEKVNIKRDM ARNPLFDTML VYHNTDVKPF EAEGLRSRLV 3070 3080 3090 3100 3110 3120 EIKRGISKFD ITVTASEAAD GLRLEVEYST TLFNKERMER LSEHLISLLE QAADHPDIAI 3130 3140 3150 3160 3170 3180 NQIDVLTKGE RHRVLYDFNR TDGVFCKEMT IPELFEKQAE KTPDHPAVAF GDETISYREL 3190 3200 3210 3220 3230 3240 NERANSLAFT LRQKGVGPDV IAGILTERSI EMIVGIMGIL KAGGAYLPID PAYPQERISY 3250 3260 3270 3280 3290 3300 IVKDSDVSVL CAAGDVDPGE AYTGDIIRID QTGQNDHVEN LKHDIKPQHL AYVIYTSGST 3310 3320 3330 3340 3350 3360 GKPKGVMIEH HSVNNLVHGL NERIYQHLDA HLNVALVAPY IFDASVKQIF AALLFGHTLC 3370 3380 3390 3400 3410 3420 IVPRETAWDA MSLIEYYSKN NINVSDMTPA HLNMLAYVDK TELEFDVKEL IVGGDALTPD 3430 3440 3450 3460 3470 3480 VIGGLFHKFP NLSCNITNVY GPTECCVDAA SHQIESGKVP QTPSIPIGRP LLNTSIYIVD 3490 3500 3510 3520 3530 3540 KELRPLPVGI AGELCIAGEG VARGYVNRPE LTAEKFVDHP FEPGKKMYKT GDLAMWLPDG 3550 3560 3570 3580 3590 3600 QIEFLGRADH QVKIRGYRIE LGEVEQQLLT HEKIKEAAVI AGKDQNGNSY LCAYIASDKE 3610 3620 3630 3640 3650 3660 LPAADVRQFL EREMPDYMIP SYFVKLDRLP RTPSGKVDRS ALPEADGNVN VMEGTGYDPP 3670 3680 3690 3700 3710 3720 RNEIERKLVQ VWREILGAED IGISHHFFAA GGDSIKALQI VSRLAKMNLK LEMKALFANP 3730 3740 3750 3760 3770 3780 KIKDLSRFIT EETRHRKHNK PVTGETELLP IQKRYFANNK EELDHFNQSF MLFRKDGYDE 3790 3800 3810 3820 3830 3840 NIVRTAFNKI LEQHDALRMI YEEKDGDIIQ YNRGYRENLF DLDVYDVRGF DSQEEKVFEL 3850 3860 3870 3880 3890 3900 ATGIQKKSSI RKGKLVHLGI FRADEGDHLL IAIHHLVVDG VSWRILFEDF ETLYLQALKG 3910 3920 3930 3940 3950 3960 EPLDIGYKTD SYQEFARQLK KYAQSRRLLK EREYWQKALE ADVPFIPAEK LERDTFEHSA 3970 3980 3990 4000 4010 4020 TLSIRIGPDV TAKLLRNAFK AYNTEINDIL LTALIAAVRD ITGENKLKVM MEGHGREDIL 4030 4040 4050 4060 4070 4080 DGVDITRTIG WFTTVYPVFI DLGEEKEISQ NIKMVKEALR KIPNKGIGYG VLKYMTEELQ 4090 4100 4110 4120 4130 4140 KIQTQAPLSF NYFGEMNNDM NRKVFSQSPF SPGESIGGKI VRHCAIEMNA ISLNGELTIY 4150 4160 4170 4180 4190 4200 TTFNQDQYQT STIEQLNQSF KENLEKIVDH CVDKEGSDMT PSDYGDVSLG LEELELIKDK 4210 4220 4230 4240 4250 4260 YSAFQIEKIY PLANMQKGML FHNAMDQTSG AYFQQIVIKL KGRVHPDILE ESFHEIVKRH 4270 4280 4290 4300 4310 4320 EILRASFEYE ITAEPRQIIA RDRKTPFTSI DLTGENRTRQ HRFIETYLKE DQEKGFDLSS 4330 4340 4350 4360 4370 4380 EALMRVCLIK MSDESYRLIW SHHHILLDGW CLGIVLSELF SLYGKIMKGE SRRLKEPKPY 4390 4400 4410 4420 4430 4440 GDYIKWLEKQ DQEEAVAYWK DYLKGYESRS ELPAFNRGAT SEEYCGKEKV ISFSKELTTK 4450 4460 4470 4480 4490 4500 ITRIAKQHHV TINTVLQGIW GMILAKYKNT DEVVFGTVVS GREAPVDGIE EMVGLFIHTI 4510 4520 4530 4540 4550 4560 PTRISFEGAR SFKEVLKKTQ AESIESNRYS YMNLSEIQVL SEMKRELITH VMAFQNYAFD 4570 4580 4590 4600 4610 4620 EELFRSQSGE TGFELEGVHG KERTNYNFNL TGVLEDEQLK LKLTFNENVY DNTIIETLEK 4630 4640 4650 4660 4670 4680 HIITVAEQVA EDETQTLRDI NLVSKEEQHR ILHTFNDTKT GYPKDKPLHE LFEEQAMKTP 4690 4700 4710 4720 4730 4740 DHTALVFGAQ RMTYRELNEK ANQTARLLRE KGIGRGSIAA IIADRSFEMI IGIIGILKAG 4750 4760 4770 4780 4790 4800 GTYLPIDPET PRDRIDYMLK NSGAALLVTT DSLLKPFDIK TVDLCSDELH LLSEENLPRV 4810 4820 4830 4840 4850 4860 NRSSDTAYIV YTSGSTGTPK GVVIPHYSAA RVVKNTNYID ITGNDVILQL SNYSFDGSVF 4870 4880 4890 4900 4910 4920 DIFGALLNGA SLVLIEKETV LNTHELAEVI KKEQVSVMFI TTALFNTLAD INIGCLAKLR 4930 4940 4950 4960 4970 4980 KILFGGERAS IPHVRKVLDH VGRDKLIHVY GPTESTVYAT YYFINEIDDE AETIPIGSPL 4990 5000 5010 5020 5030 5040 ANTSVLIMDE AGKLLPIGVP GELCIAGDGL SKGYLNREEL TAEKFIPHPF IPGERLYKTG 5050 5060 5070 5080 5090 5100 DLAKWLPDGN IEFIGRIDHQ VKIRGFRIEL GEIESRLEMH EDINETIVTV REDEESRPYI 5110 5120 5130 5140 5150 5160 CAYITANREI SLDELKGFLG EKLPDYMIPA YFVKMDKLPL TKNGKVDRKA LPEPDRSAGT 5170 5180 5190 5200 5210 5220 EAEYEAPRNY VEQRIISILE DVLGTERMGI SCHFFDKGGN SLKAMQAVHS INKTFGIDMR 5230 5240 5250 ISTFFKHPTA KSLARFVLTA EAESAVSEEY AEEEV

References

[1]	"The bacitracin biosynthesis operon of Bacillus licheniformis ATCC 10716: molecular characterization of three multi-modular peptide synthetases." Konz D., Klens A., Schoergendorfer K., Marahiel M.A. Chem. Biol. 4:927-937(1997) [PubMed: 9427658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10716 / DSM 603 / NBRC 12199 / NCIMB 8874.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007865 Genomic DNA. Translation: AAC06346.1.
PIR	T31677.
3D structure databases
ProteinModelPortal	O68006.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR001242. Condensatn. IPR013624. NRPS. IPR010060. NRPS_synth. IPR006163. Phsphopanteth-bd. IPR006162. PPantetheine_attach_site. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 5 hits.
Pfam	PF00501. AMP-binding. 5 hits. PF00668. Condensation. 5 hits. PF08415. NRPS. 1 hit. PF00550. PP-binding. 5 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 5 hits.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 5 hits. TIGR01720. NRPS-para261. 1 hit.
PROSITE	PS50075. ACP_DOMAIN. 5 hits. PS00455. AMP_BINDING. 5 hits. PS00012. PHOSPHOPANTETHEINE. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BACA_BACLI

Accession

Primary (citable) accession number: O68006

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	September 21, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program