Chlorocatechol 1,2-dioxygenase - Rhodococcus opacus

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Reviewed, UniProtKB/Swiss-Prot O67987 (CLCA_RHOOP)

Last modified February 9, 2010. Version 50. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Chlorocatechol 1,2-dioxygenase
EC=1.13.11.-

Gene names

Name:

clcA

Organism

Rhodococcus opacus (Nocardia opaca)

Taxonomic identifier

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity

4-chlorocatechol + O₂ = 3-chloro-muconate.

3,5-dichlorocatechol + O₂ = 2,4-dichloro -muconate.

Cofactor

Binds 1 Fe³⁺ ion per subunit.

Sequence similarities

Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro chlorocatechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

257

Chlorocatechol 1,2-dioxygenase

PRO_0000085088

Sites

Metal binding

1

Iron By similarity

Metal binding

1

Iron By similarity

Metal binding

1

Iron By similarity

Metal binding

1

Iron By similarity

Secondary structure

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257

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

O67987-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D834A96E85A15777
Show »

257

28,953

        10         20         30         40         50         60 
MANTRVIELF DEFTDLIRDF IVRHEITTPE YETIMQYMIS VGEAGEWPLW LDAFFETTVD 

        70         80         90        100        110        120 
SVSYGKGNWT SSAIQGPFFK EGAPLLTGKP ATLPMRADEP GDRMRFTGSV RDTSGTPITG 

       130        140        150        160        170        180 
AVIDVWHSTN DGNYSFFSPA LPDQYLLRGR VVPAEDGSIE FHSIRPVPYE IPKAGPTGQL 

       190        200        210        220        230        240 
MNSYLGRHSW RPAHIHIRIT ADGYRPLITQ LYFEGDPYLD SDSCSAVKSE LVLPVNKIDI 

       250 
DGETWQLVDF NFILQHN

References

[1]	"Evolutionary relationship between chlorocatechol catabolic enzymes from Rhodococcus opacus 1CP and their counterparts in proteobacteria: sequence divergence and functional convergence." Eulberg D., Kourbatova E.M., Golovleva L.A., Schloemann M. J. Bacteriol. 180:1082-1094(1998) [PubMed: 9495745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1CP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF003948 Genomic DNA. Translation: AAC38251.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S9A	X-ray	2.47	A/B	1-257	[»]

ModBase

Family and domain databases

InterPro

IPR007535. Catechol_dOase_N.
IPR012817. Chlorcchol_dOase.
IPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
[Graphical view]

Gene3D

G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.

Pfam

PF00775. Dioxygenase_C. 1 hit.
PF04444. Dioxygenase_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02465. chlorocat_1_2. 1 hit.

PROSITE

PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

CLCA_RHOOP

Accession

Primary (citable) accession number: O67987

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents