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Protein

Molybdopterin synthase catalytic subunit

Gene

moaE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD (By similarity).By similarity

Catalytic activityi

Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H2O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein].

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1563-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Molybdopterin synthase catalytic subunit (EC:2.8.1.12)
Alternative name(s):
MPT synthase subunit 2
Molybdenum cofactor biosynthesis protein E
Molybdopterin-converting factor large subunit
Molybdopterin-converting factor subunit 2
Gene namesi
Name:moaE
Ordered Locus Names:aq_2181
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Molybdopterin synthase catalytic subunitPRO_0000163076Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis (By similarity).By similarity

Protein-protein interaction databases

STRINGi224324.aq_2181.

Structurei

Secondary structure

1
154
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147Combined sources
Helixi18 – 247Combined sources
Beta strandi33 – 397Combined sources
Helixi45 – 473Combined sources
Beta strandi51 – 588Combined sources
Helixi60 – 7819Combined sources
Beta strandi81 – 888Combined sources
Beta strandi90 – 934Combined sources
Beta strandi99 – 1079Combined sources
Helixi108 – 12518Combined sources
Beta strandi128 – 1347Combined sources
Helixi138 – 1403Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OMDX-ray2.00A/B1-154[»]
ProteinModelPortaliO67928.
SMRiO67928. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67928.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 423Substrate bindingBy similarity
Regioni107 – 1082Substrate bindingBy similarity
Regioni130 – 1323Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the MoaE family.Curated

Phylogenomic databases

eggNOGiENOG4108ZAF. Bacteria.
COG0314. LUCA.
HOGENOMiHOG000280877.
InParanoidiO67928.
KOiK03635.
OMAiGEICLFV.
OrthoDBiPOG091H0631.

Family and domain databases

CDDicd00756. MoaE. 1 hit.
Gene3Di3.90.1170.40. 1 hit.
InterProiIPR003448. Mopterin_biosynth_MoaE.
[Graphical view]
PANTHERiPTHR23404:SF2. PTHR23404:SF2. 1 hit.
PfamiPF02391. MoaE. 1 hit.
[Graphical view]
SUPFAMiSSF54690. SSF54690. 1 hit.

Sequencei

Sequence statusi: Complete.

O67928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVGMIPRVY LGHEWFGAER ILSEYQVPED CGAQVLFLGI PRNAPEDGGN
60 70 80 90 100
IEALEYEAYP EMAIKEMEKI RQETIEKFGV KEVFIHHRLG LVKIGEPSFL
110 120 130 140 150
VLAVGGHREE TFKACRYAVD ETKKRVPIWK KEIFKEGKGE WVLGEKKNAS

GQTK
Length:154
Mass (Da):17,579
Last modified:August 1, 1998 - v1
Checksum:i84BDCBD4FD54DF34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07878.1.
PIRiD70487.
RefSeqiNP_214497.1. NC_000918.1.
WP_010881433.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07878; AAC07878; aq_2181.
GeneIDi1193917.
KEGGiaae:aq_2181.
PATRICi20961194. VBIAquAeo85532_1687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07878.1.
PIRiD70487.
RefSeqiNP_214497.1. NC_000918.1.
WP_010881433.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OMDX-ray2.00A/B1-154[»]
ProteinModelPortaliO67928.
SMRiO67928. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07878; AAC07878; aq_2181.
GeneIDi1193917.
KEGGiaae:aq_2181.
PATRICi20961194. VBIAquAeo85532_1687.

Phylogenomic databases

eggNOGiENOG4108ZAF. Bacteria.
COG0314. LUCA.
HOGENOMiHOG000280877.
InParanoidiO67928.
KOiK03635.
OMAiGEICLFV.
OrthoDBiPOG091H0631.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciAAEO224324:GJBH-1563-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO67928.

Family and domain databases

CDDicd00756. MoaE. 1 hit.
Gene3Di3.90.1170.40. 1 hit.
InterProiIPR003448. Mopterin_biosynth_MoaE.
[Graphical view]
PANTHERiPTHR23404:SF2. PTHR23404:SF2. 1 hit.
PfamiPF02391. MoaE. 1 hit.
[Graphical view]
SUPFAMiSSF54690. SSF54690. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOAE_AQUAE
AccessioniPrimary (citable) accession number: O67928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.