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Protein

Shikimate kinase

Gene

aroK

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14MagnesiumUniRule annotation1
Binding sitei32SubstrateUniRule annotation1
Binding sitei56SubstrateUniRule annotation1
Binding sitei78Substrate; via amide nitrogenUniRule annotation1
Binding sitei116ATPUniRule annotation1
Binding sitei133SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:aq_2177
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001923661 – 168Shikimate kinaseAdd BLAST168

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_2177.

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi13 – 24Combined sources12
Beta strandi28 – 30Combined sources3
Helixi31 – 39Combined sources9
Helixi43 – 49Combined sources7
Helixi52 – 66Combined sources15
Beta strandi69 – 75Combined sources7
Helixi78 – 81Combined sources4
Helixi84 – 91Combined sources8
Beta strandi94 – 100Combined sources7
Helixi103 – 109Combined sources7
Helixi118 – 120Combined sources3
Helixi123 – 125Combined sources3
Helixi127 – 137Combined sources11
Beta strandi140 – 145Combined sources6
Helixi150 – 162Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PT5X-ray2.10A/B/C/D1-168[»]
ProteinModelPortaliO67925.
SMRiO67925.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67925.

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiO67925.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIYLIGFMC SGKSTVGSLL SRSLNIPFYD VDEEVQKREG LSIPQIFEKK
60 70 80 90 100
GEAYFRKLEF EVLKDLSEKE NVVISTGGGL GANEEALNFM KSRGTTVFID
110 120 130 140 150
IPFEVFLERC KDSKERPLLK RPLDEIKNLF EERRKIYSKA DIKVKGEKPP
160
EEVVKEILLS LEGNALGG
Length:168
Mass (Da):19,080
Last modified:August 1, 1998 - v1
Checksum:iAB1500BC484DEDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07875.1.
PIRiA70487.
RefSeqiNP_214494.1. NC_000918.1.
WP_010881430.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07875; AAC07875; aq_2177.
GeneIDi1193914.
KEGGiaae:aq_2177.
PATRICi20961186. VBIAquAeo85532_1683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07875.1.
PIRiA70487.
RefSeqiNP_214494.1. NC_000918.1.
WP_010881430.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PT5X-ray2.10A/B/C/D1-168[»]
ProteinModelPortaliO67925.
SMRiO67925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07875; AAC07875; aq_2177.
GeneIDi1193914.
KEGGiaae:aq_2177.
PATRICi20961186. VBIAquAeo85532_1683.

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiO67925.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Miscellaneous databases

EvolutionaryTraceiO67925.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROK_AQUAE
AccessioniPrimary (citable) accession number: O67925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.