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Protein

Shikimate kinase

Gene

aroK

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141MagnesiumUniRule annotation
Binding sitei32 – 321SubstrateUniRule annotation
Binding sitei56 – 561SubstrateUniRule annotation
Binding sitei78 – 781Substrate; via amide nitrogenUniRule annotation
Binding sitei116 – 1161ATPUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1560-MONOMER.
UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:aq_2177
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Shikimate kinasePRO_0000192366Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_2177.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi13 – 2412Combined sources
Beta strandi28 – 303Combined sources
Helixi31 – 399Combined sources
Helixi43 – 497Combined sources
Helixi52 – 6615Combined sources
Beta strandi69 – 757Combined sources
Helixi78 – 814Combined sources
Helixi84 – 918Combined sources
Beta strandi94 – 1007Combined sources
Helixi103 – 1097Combined sources
Helixi118 – 1203Combined sources
Helixi123 – 1253Combined sources
Helixi127 – 13711Combined sources
Beta strandi140 – 1456Combined sources
Helixi150 – 16213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PT5X-ray2.10A/B/C/D1-168[»]
ProteinModelPortaliO67925.
SMRiO67925. Positions 1-168.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67925.

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiO67925.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIYLIGFMC SGKSTVGSLL SRSLNIPFYD VDEEVQKREG LSIPQIFEKK
60 70 80 90 100
GEAYFRKLEF EVLKDLSEKE NVVISTGGGL GANEEALNFM KSRGTTVFID
110 120 130 140 150
IPFEVFLERC KDSKERPLLK RPLDEIKNLF EERRKIYSKA DIKVKGEKPP
160
EEVVKEILLS LEGNALGG
Length:168
Mass (Da):19,080
Last modified:August 1, 1998 - v1
Checksum:iAB1500BC484DEDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07875.1.
PIRiA70487.
RefSeqiNP_214494.1. NC_000918.1.
WP_010881430.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07875; AAC07875; aq_2177.
GeneIDi1193914.
KEGGiaae:aq_2177.
PATRICi20961186. VBIAquAeo85532_1683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07875.1.
PIRiA70487.
RefSeqiNP_214494.1. NC_000918.1.
WP_010881430.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PT5X-ray2.10A/B/C/D1-168[»]
ProteinModelPortaliO67925.
SMRiO67925. Positions 1-168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07875; AAC07875; aq_2177.
GeneIDi1193914.
KEGGiaae:aq_2177.
PATRICi20961186. VBIAquAeo85532_1683.

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiO67925.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.
BioCyciAAEO224324:GJBH-1560-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO67925.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROK_AQUAE
AccessioniPrimary (citable) accession number: O67925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.