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O67911

- O67911_AQUAE

UniProt

O67911 - O67911_AQUAE

Protein
Submitted name:

Poly A polymerase

Gene

pcnB2

Organism
Aquifex aeolicus (strain VF5)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451CTPImported
    Binding sitei58 – 581CTPImported
    Binding sitei137 – 1371CTP; via carbonyl oxygenImported
    Binding sitei222 – 2221CTPImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi132 – 1332CTPImported
    Nucleotide bindingi182 – 1887CTPImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: InterPro
    3. nucleotidyltransferase activity Source: UniProtKB-KW
    4. phosphoric diester hydrolase activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    NucleotidyltransferaseSAAS annotation, Transferase

    Keywords - Ligandi

    ATP-bindingSAAS annotation, Nucleotide-binding, RNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1545-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Poly A polymeraseImported
    Gene namesi
    Name:pcnB2Imported
    Ordered Locus Names:aq_2158Imported
    OrganismiAquifex aeolicus (strain VF5)Imported
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi224324.aq_2158.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WFOX-ray3.40A/B/C1-512[»]
    3WFPX-ray4.00A/B/C/D/E/F/G/H17-450[»]
    3WFQX-ray3.62E/F/G/H16-448[»]
    3WFRX-ray3.50E/F/G/H16-453[»]
    3WFSX-ray3.31C/D16-448[»]
    ProteinModelPortaliO67911.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000062915.
    KOiK00970.
    OMAiKDVYAHS.
    OrthoDBiEOG64R655.

    Family and domain databases

    InterProiIPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002646. PolA_pol_head_dom.
    IPR006675. Uncharacterised_HDIG.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    PF01743. PolyA_pol. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00277. HDIG. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O67911-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENIEIVSSG KHTLHGLNFY LSYFDDVAKV LPREHYCFIV GGWVRDRILG    50
    EPVGYNIDVD FLTTADPVEL AKNFAKRIGG HFFVFEKRGF LIKRPTIASV 100
    VLHLPPYRYR FDFSPLKGKD LEKALIEDLK ERDFTANAIA VNLDDVLSIG 150
    AKQTIVYDPT GGIKDLEQGL LRPVSIENLK RDPVRVLRGF RIAIEKNLQL 200
    TEDFYEFVKE DPRIVLKSAV ERITHELFKI MKEKTAHKVI RELYEYGVLE 250
    AIIPEIGRLR EVKDQGEHHI YPLDEHTLKT LEYLEQVIED RAKYLSAELL 300
    ENFGKKRVLG EFTDVELLKW GALFHDIGKP QTFAVREGKV TFYEHDKVGA 350
    QIVREIGERL RWGDEATEFV AKLVRHHLRP FFLREAFKKG ELKRRGMANF 400
    WRECGDIAPH LFLLSIADAM ASGDEEEDIK ALMETIAELE SFNRNEMKEE 450
    IQKPLLNGDE IMEILGIKPG KIVGILKKAL LEAQIDGKVE TKEEAIEFIK 500
    RSTKNLKPLD EG 512
    Length:512
    Mass (Da):58,913
    Last modified:August 1, 1998 - v1
    Checksum:i58CDB8FD1A1B6F0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07883.1.
    PIRiC70485.
    RefSeqiNP_214480.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07883; AAC07883; aq_2158.
    GeneIDi1193900.
    KEGGiaae:aq_2158.
    PATRICi20961154. VBIAquAeo85532_1668.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07883.1 .
    PIRi C70485.
    RefSeqi NP_214480.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WFO X-ray 3.40 A/B/C 1-512 [» ]
    3WFP X-ray 4.00 A/B/C/D/E/F/G/H 17-450 [» ]
    3WFQ X-ray 3.62 E/F/G/H 16-448 [» ]
    3WFR X-ray 3.50 E/F/G/H 16-453 [» ]
    3WFS X-ray 3.31 C/D 16-448 [» ]
    ProteinModelPortali O67911.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_2158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07883 ; AAC07883 ; aq_2158 .
    GeneIDi 1193900.
    KEGGi aae:aq_2158.
    PATRICi 20961154. VBIAquAeo85532_1668.

    Phylogenomic databases

    HOGENOMi HOG000062915.
    KOi K00970.
    OMAi KDVYAHS.
    OrthoDBi EOG64R655.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-1545-MONOMER.

    Family and domain databases

    InterProi IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002646. PolA_pol_head_dom.
    IPR006675. Uncharacterised_HDIG.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    PF01743. PolyA_pol. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00277. HDIG. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5Imported.
    2. "Translocation and Rotation of tRNA during Template-Independent RNA Polymerization by tRNA Nucleotidyltransferase."
      Yamashita S., Takeshita D., Tomita K.
      Submitted (JUL-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 16-453 IN COMPLEX WITH CTP.

    Entry informationi

    Entry nameiO67911_AQUAE
    AccessioniPrimary (citable) accession number: O67911
    Entry historyi
    Integrated into UniProtKB/TrEMBL: August 1, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3