O67899 (PPSA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate synthase
Short name=PEP synthase
EC=2.7.9.2

Alternative name(s):
Pyruvate, water dikinase

Gene names

Name:	ppsA
Ordered Locus Names:	aq_2142

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Phosphoenolpyruvate synthase		PRO_0000147032
Sites
	Active site	433	1	Tele-phosphohistidine intermediate By similarity
	Active site	809	1	Proton donor By similarity
	Metal binding	738	1	Magnesium By similarity
	Metal binding	762	1	Magnesium By similarity
	Binding site	523	1	Substrate By similarity
	Binding site	636	1	Substrate By similarity
	Binding site	738	1	Substrate By similarity
	Binding site	759	1	Substrate; via carbonyl oxygen By similarity
	Binding site	760	1	Substrate; via amide nitrogen By similarity
	Binding site	761	1	Substrate By similarity
	Binding site	762	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67899 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 0CB001DABE289912	FASTA	856	96,407
	10 20 30 40 50 60 MSVDKSKALV LWLDEVTIED IPIAGGKNAS LGEMIRNLSP LGVKIPYGYV VTANAYYYFL 70 80 90 100 110 120 DYNNLRDKIR KILEGLNTDD LKDLQRRGHE VRELIRGGTF PPDLEEAIKD YYNKLSEKYK 130 140 150 160 170 180 THAVDVAVRS SATAEDLPDA SFAGQQETYL NVVGAENVLV AIKNCFASLF TDRAIVYRER 190 200 210 220 230 240 FGFDHFKVGI AVGVQKMVRS DMGASGVMFT LDTETGFKDV VVINAAYGLG ELLVRGEVTP 250 260 270 280 290 300 DEYIVFKPTL MKGYSAIIEK KLGRKDRKMI YGTGDERVKI VNVPKEDQKK FALNDDEILQ 310 320 330 340 350 360 LAKWGVLIEE HYSKKNGRWT PMDIEWAKDG ILNELFVVQA RPETVHSRKK ENVVKIYKIK 370 380 390 400 410 420 TPEEERKNRV IVKGIAVGDK IATGKARVLF DLKEADQFQE GEILVTDITD PDWEPVMKKA 430 440 450 460 470 480 AAIVTNRGGR TSHAAIVARE LGIPAVVGTG NATEKIKTGE EITVSCAEGE TGYVYEGKID 490 500 510 520 530 540 YEVEEINLEN IPKPKTKIMM NIGNPESAFR YASLPNDGVG LAREEFIIAN YIKIHPLALL 550 560 570 580 590 600 HYEDLKELYE KLERENLIDE KGFVQFKLIY HYANGRLANK LAKGKDKLRV NLRKILQDIE 610 620 630 640 650 660 NLTFGYEDKA TYYIKKLSYG IAKIAAAFYP NPVIVRFSDF KSNEYANLIG GILFEPEEEN 670 680 690 700 710 720 PMLGWRGASR YYSDVFKEAF GMECKAIIRV RNKMGLTNTK VMIPFCRTPE EGEKVLQVME 730 740 750 760 770 780 EYGLRKGENG LEVYVMAELP SNIVLADRYA QIFDGFSIGS NDLTQLTLGL DRDSELVAHL 790 800 810 820 830 840 YDERNEAVKR LIAQLIKTAK EYGRKVGICG QAPSDFPEFA QFLVEQGIDS ISLNPDSVLK 850 TMLAVVEMEK KLGVLK

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07865.1.
PIR	G70483.
RefSeq	NP_214468.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67899.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193888.
GenomeReviews	Gene locus aq_2142 in contig AE000657_GR.
KEGG	aae:aq_2142.
NMPDR	fig|224324.1.peg.1483.
PATRIC	20961126. VBIAquAeo85532_1656.
Phylogenomic databases
HOGENOM	HBG284863.
OMA	INNHIKA.
PhylomeDB	O67899.
ProtClustDB	PRK06464.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_2142-MONOMER.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR008279. PEP-util_enz_mobile_dom. IPR006319. PEP_synth. IPR018274. PEP_util_AS. IPR000121. PEP_util_C. IPR023151. PEP_util_CS. IPR006318. PEP_util_enz. IPR002192. PPDK_PEP-bd. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 2 hits.
KO	K01007.
Pfam	PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
SUPFAM	SSF52009. PEP_mobile. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01418. PEP_synth. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_AQUAE

Accession

Primary (citable) accession number: O67899

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program