Reviewed, UniProtKB/Swiss-Prot O67886 (HEMN_AQUAE)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	aq_2124

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Oxygen-independent coproporphyrinogen III oxidase		PRO_0000109938
Regions
	Region	113 – 114	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	61	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	65	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	68	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	55	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	67	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	112	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	145	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	172	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	184	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	209	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67886-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 4E494103B8EF6342	FASTA	456	53,785
	10 20 30 40 50 60 MKAEFDKELI KKYDRPGPRY TSYPPATEFT EEVKEDEYVK RLIKSNERKT PLSLYFHIPF 70 80 90 100 110 120 CEQRCLYCGC NVIISHRKGI EEPYLERVCR EMDLVSQYLD KDRKVIQLHW GGGTPNYLSP 130 140 150 160 170 180 EQIKWFMEEI RKRFEFGDNA EISIELDPRY LTDEQIKAIK DAGFNRISLG VQDLDPKVQQ 190 200 210 220 230 240 AVNRVQPYEL IKEKMEKLRE AGFESINLDL IYGLPYQTKE SFEKTVEKVI ELNPDRIATY 250 260 270 280 290 300 SFAYIPQVKP HQQLLPKEAL PSAEEKLRIF EMVINKFQEA GYVYIGMDHF AKPEDELAVA 310 320 330 340 350 360 QRKGELWRNF QGYTTKKGVE LLGFGATSIG MLYDSYFQNW KTLRDYNKTV DEGKIPVFRG 370 380 390 400 410 420 YVLNEDDFIR REVIMDIMCN LGVEFSKIEN MFGINFREYF AKELEELKEM EEDGLIKVEE 430 440 450 DRIKIMPVGR LLIRNVAMVF DAHLRRKKEL NFSRTI

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07846.1.
PIR	B70482.
RefSeq	NP_214455.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1193875.
GenomeReviews	Gene locus aq_2124 in contig AE000657_GR.
KEGG	aae:aq_2124.
NMPDR	fig|224324.1.peg.1470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67886.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_2124-MON.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEMN_AQUAE

Accession

Primary (citable) accession number: O67886

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)