O67883 (O67883_AQUAE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Thiamine monophosphate kinase EMBL AAC07848.1

Gene names

Name:	thiL EMBL AAC07848.1
Ordered Locus Names:	aq_2119

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	ATP-binding PDB 3C9R Magnesium PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T Metal-binding PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T Nucleotide-binding Thiamine pyrophosphate PDB 3C9U
Molecular function	Kinase EMBL AAC07848.1 Transferase
Technical term	3D-structure PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T PDB 1VQV Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine-phosphate kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	25 – 27	3	ADP PDB 3C9U
	Nucleotide binding	25 – 26	2	ATP PDB 3C9R
	Nucleotide binding	117 – 119	3	ADP PDB 3C9U
	Nucleotide binding	117 – 119	3	ATP PDB 3C9R
	Nucleotide binding	209 – 210	2	ATP PDB 3C9R
	Region	48 – 50	3	Thiamine pyrophosphate binding PDB 3C9U
	Region	209 – 210	2	Thiamine pyrophosphate binding PDB 3C9U
Sites
	Metal binding	27	1	Magnesium PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T
	Metal binding	41	1	Magnesium PDB 3C9U
	Metal binding	42	1	Magnesium; via carbonyl oxygen PDB 3C9S
	Metal binding	43	1	Magnesium PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T
	Metal binding	71	1	Magnesium PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T
	Metal binding	119	1	Magnesium PDB 3C9U PDB 3C9S
	Metal binding	207	1	Magnesium PDB 3C9R PDB 3C9U PDB 3C9S PDB 3C9T
	Metal binding	210	1	Magnesium PDB 3C9U PDB 3C9T
	Metal binding	215	1	Magnesium PDB 3C9T
	Metal binding	218	1	Magnesium; via tele nitrogen PDB 3C9T
	Binding site	43	1	ADP PDB 3C9U
	Binding site	43	1	ATP PDB 3C9R
	Binding site	43	1	Thiamine pyrophosphate PDB 3C9U
	Binding site	71	1	ADP PDB 3C9U
	Binding site	71	1	ATP PDB 3C9R
	Binding site	101	1	ADP PDB 3C9U
	Binding site	101	1	ATP PDB 3C9R
	Binding site	142	1	ADP PDB 3C9U
	Binding site	142	1	ATP PDB 3C9R
	Binding site	260	1	Thiamine pyrophosphate PDB 3C9U
	Binding site	303	1	Thiamine pyrophosphate PDB 3C9U

Sequences

	Sequence		Length	Mass (Da)
	O67883 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B5E5E63489D9BB32	FASTA	306	34,413
	10 20 30 40 50 60 MRLKELGEFG LIDLIKKTLE SKVIGDDTAP VEYCSKKLLL TTDVLNEGVH FLRSYIPEAV 70 80 90 100 110 120 GWKAISVNVS DVIANGGLPK WALISLNLPE DLEVSYVERF YIGVKRACEF YKCEVVGGNI 130 140 150 160 170 180 SKSEKIGISV FLVGETERFV GRDGARLGDS VFVSGTLGDS RAGLELLLME KEEYEPFELA 190 200 210 220 230 240 LIQRHLRPTA RIDYVKHIQK YANASMDISD GLVADANHLA QRSGVKIEIL SEKLPLSNEL 250 260 270 280 290 300 KMYCEKYGKN PIEYALFGGE DYQLLFTHPK ERWNPFLDMT EIGRVEEGEG VFVDGKKVEP KGWKHF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
[2]	"Structural studies of thiamin monophosphate kinase in complex with substrates and products." McCulloch K.M., Kinsland C., Begley T.P., Ealick S.E. Biochemistry 47:3810-3821(2008) [PubMed: 18311927] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.48 ANGSTROMS) IN COMPLEX WITH ADP; ATP; MAGNESIUM AND THIAMINE PYROPHOSPHATE.
[3]	"Crystal Structure of Thiamine Monophosphate Kinase (Thil) from Aquifex Aeolicus." Eswaramoorthy S., Swaminathan S. Submitted (JAN-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC07848.1.

PIR

G70481.

RefSeq

NP_214452.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VQV	X-ray	2.65	A/B	1-306	[»]
3C9R	X-ray	2.30	A/B	1-306	[»]
3C9S	X-ray	2.20	A/B	1-306	[»]
3C9T	X-ray	2.60	A/B	1-306	[»]
3C9U	X-ray	1.48	A/B	1-306	[»]

ProteinModelPortal

O67883.

SMR

O67883. Positions 1-306.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1193872.

GenomeReviews

Gene locus aq_2119 in contig AE000657_GR.

KEGG

aae:aq_2119.

NMPDR

fig|224324.1.peg.1467.

PATRIC

20961084. VBIAquAeo85532_1635.

Phylogenomic databases

HOGENOM

HBG577902.

OMA

YFAPLSG.

PhylomeDB

O67883.

ProtClustDB

CLSK2299941.

Family and domain databases

InterPro

IPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR016188. PurM_N-like.
IPR006283. ThiL.
[Graphical view]

KO

K00946.

Pfam

PF00586. AIRS. 1 hit.
[Graphical view]

PIRSF

PIRSF005303. Thiam_monoph_kin. 1 hit.

SUPFAM

SSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.

TIGRFAMs

TIGR01379. ThiL. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O67883_AQUAE

Accession

Primary (citable) accession number: O67883

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)