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Protein

Methylthioribose-1-phosphate isomerase

Gene

mtnA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).By similarity

Catalytic activityi

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (mtnB)
  3. Enolase-phosphatase E1 (mtnC)
  4. Enolase-phosphatase E1 (mtnC)
  5. Acireductone dioxygenase (mtnD)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211SubstrateBy similarity1
Sitei270Transition state stabilizerBy similarity1
Binding sitei309SubstrateBy similarity1
Active sitei350Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00904; UER00874.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose-1-phosphate isomerase (EC:5.3.1.23)
Short name:
M1Pi
Short name:
MTR-1-P isomerase
Alternative name(s):
S-methyl-5-thioribose-1-phosphate isomerase
Gene namesi
Name:mtnA
Ordered Locus Names:aq_2114
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560911 – 456Methylthioribose-1-phosphate isomeraseAdd BLAST456

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_2114.

Structurei

3D structure databases

ProteinModelPortaliO67879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 113ThioesteraseAdd BLAST74
Regioni124 – 456MTR-1-P isomeraseAdd BLAST333
Regioni176 – 178Substrate bindingBy similarity3
Regioni360 – 361Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C35. Bacteria.
COG0182. LUCA.
COG2050. LUCA.
HOGENOMiHOG000224730.
InParanoidiO67879.
KOiK08963.
OMAiFYNPAFD.
OrthoDBiPOG091H022L.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
3.10.129.10. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 1 hit.
PF01008. IF-2B. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.

Sequencei

Sequence statusi: Complete.

O67879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIRTHLGIN QELSGKPVKV AEGYAEVLLK TQESMKADDK GLVHGGFIFS
60 70 80 90 100
AADYASMLAV NHPNVVLAGA NVKFLKPVKV GDEIICKARV SEDKGKKKKV
110 120 130 140 150
LVECFKGEDK VFEGEFFTVF LIDMFWRGEK MEVKPFYWKG DKLLLLDQRK
160 170 180 190 200
LPHQEVWLEL KTYEEVAKAI KEMAVRGAPA IGCTAAYGFV LGVKVQKEDP
210 220 230 240 250
EKVYETLKNT RPTAYNLFWA LDRMMKALKE GKDIEEEAKE IEKEDYEANK
260 270 280 290 300
RMGEIGSEIV PLNAKVLTHC NTGALATAGW GTALGVIRSA HYGGKNIFVW
310 320 330 340 350
VDETRPYLQG ARLTAWELVK ESIPHKIITD STAGFLMKKG MVDLVIVGAD
360 370 380 390 400
RITAKGDVAN KIGTYTLAVL CKEHNVPFYV AAPTSTIDSN IKSGEEIIIE
410 420 430 440 450
ERSPEEVKVC GGCAIAPKES DALHLAFDIT PAELITGIIT EKGIFKPERI

TEALKD
Length:456
Mass (Da):50,498
Last modified:August 1, 1998 - v1
Checksum:i477F3F6B4BB6E96E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07840.1.
PIRiC70481.
RefSeqiNP_214448.1. NC_000918.1.
WP_010881384.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07840; AAC07840; aq_2114.
GeneIDi1193868.
KEGGiaae:aq_2114.
PATRICi20961076. VBIAquAeo85532_1631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07840.1.
PIRiC70481.
RefSeqiNP_214448.1. NC_000918.1.
WP_010881384.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67879.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07840; AAC07840; aq_2114.
GeneIDi1193868.
KEGGiaae:aq_2114.
PATRICi20961076. VBIAquAeo85532_1631.

Phylogenomic databases

eggNOGiENOG4105C35. Bacteria.
COG0182. LUCA.
COG2050. LUCA.
HOGENOMiHOG000224730.
InParanoidiO67879.
KOiK08963.
OMAiFYNPAFD.
OrthoDBiPOG091H022L.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00874.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
3.10.129.10. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 1 hit.
PF01008. IF-2B. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTNA_AQUAE
AccessioniPrimary (citable) accession number: O67879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.