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O67876

- HEM2_AQUAE

UniProt

O67876 - HEM2_AQUAE

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi123 – 1231Zinc; catalyticBy similarity
    Metal bindingi125 – 1251Zinc; catalyticBy similarity
    Metal bindingi133 – 1331Zinc; catalyticBy similarity
    Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
    Binding sitei208 – 2081Substrate 1By similarity
    Binding sitei220 – 2201Substrate 1By similarity
    Metal bindingi236 – 2361MagnesiumBy similarity
    Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
    Binding sitei277 – 2771Substrate 2By similarity
    Binding sitei316 – 3161Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1508-MONOMER.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:aq_2109
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Delta-aminolevulinic acid dehydratasePRO_0000140491Add
    BLAST

    Proteomic databases

    PRIDEiO67876.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi224324.aq_2109.

    Structurei

    3D structure databases

    ProteinModelPortaliO67876.
    SMRiO67876. Positions 9-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiDMILTYF.
    OrthoDBiEOG6VXFCB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67876-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVGRFPSSR PRRLRRNENI RRLVRETHLT LDDLIYPIFV RWGENVVEEV    50
    PSMPGVYRYS VDKVVDAVKE VRDLGIPAII LFGIPEHKDE VGSDTWSEEG 100
    IIQRALRKIK KEVPDIYVIT DVCFCEYTTH GHCGVLHDHD VENDLTLENL 150
    KKQVVSHAEN GADMVAPSGM MDGMVKVIRE ALDEAGFKHI PIMAYSAKFA 200
    SAYYGPFREA AESAPAFGDR RTYQMDPANS REALKEVLMD VEEGADVVMV 250
    KPALAYLDII AKVKENVLVP VCAYNVSGEY SLIKAAGKLG WIDEKKVMWE 300
    TLLSIKRAGA DMIITYFAKD AARMIIEGEV 330
    Length:330
    Mass (Da):37,091
    Last modified:August 1, 1998 - v1
    Checksum:iAC70D2063D9C7457
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07837.1.
    PIRiH70480.
    RefSeqiNP_214445.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07837; AAC07837; aq_2109.
    GeneIDi1193865.
    KEGGiaae:aq_2109.
    PATRICi20961068. VBIAquAeo85532_1627.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07837.1 .
    PIRi H70480.
    RefSeqi NP_214445.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67876.
    SMRi O67876. Positions 9-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_2109.

    Proteomic databases

    PRIDEi O67876.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07837 ; AAC07837 ; aq_2109 .
    GeneIDi 1193865.
    KEGGi aae:aq_2109.
    PATRICi 20961068. VBIAquAeo85532_1627.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi DMILTYF.
    OrthoDBi EOG6VXFCB.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    BioCyci AAEO224324:GJBH-1508-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiHEM2_AQUAE
    AccessioniPrimary (citable) accession number: O67876
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3