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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathway:iprotoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231Zinc; catalyticBy similarity
Metal bindingi125 – 1251Zinc; catalyticBy similarity
Metal bindingi133 – 1331Zinc; catalyticBy similarity
Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
Binding sitei208 – 2081Substrate 1By similarity
Binding sitei220 – 2201Substrate 1By similarity
Metal bindingi236 – 2361MagnesiumBy similarity
Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
Binding sitei277 – 2771Substrate 2By similarity
Binding sitei316 – 3161Substrate 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1508-MONOMER.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:aq_2109
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Delta-aminolevulinic acid dehydratasePRO_0000140491Add
BLAST

Proteomic databases

PRIDEiO67876.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_2109.

Structurei

3D structure databases

ProteinModelPortaliO67876.
SMRiO67876. Positions 9-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
InParanoidiO67876.
KOiK01698.
OMAiSTYQMDP.
OrthoDBiEOG6VXFCB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVGRFPSSR PRRLRRNENI RRLVRETHLT LDDLIYPIFV RWGENVVEEV
60 70 80 90 100
PSMPGVYRYS VDKVVDAVKE VRDLGIPAII LFGIPEHKDE VGSDTWSEEG
110 120 130 140 150
IIQRALRKIK KEVPDIYVIT DVCFCEYTTH GHCGVLHDHD VENDLTLENL
160 170 180 190 200
KKQVVSHAEN GADMVAPSGM MDGMVKVIRE ALDEAGFKHI PIMAYSAKFA
210 220 230 240 250
SAYYGPFREA AESAPAFGDR RTYQMDPANS REALKEVLMD VEEGADVVMV
260 270 280 290 300
KPALAYLDII AKVKENVLVP VCAYNVSGEY SLIKAAGKLG WIDEKKVMWE
310 320 330
TLLSIKRAGA DMIITYFAKD AARMIIEGEV
Length:330
Mass (Da):37,091
Last modified:August 1, 1998 - v1
Checksum:iAC70D2063D9C7457
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07837.1.
PIRiH70480.
RefSeqiNP_214445.1. NC_000918.1.
WP_010881381.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07837; AAC07837; aq_2109.
GeneIDi1193865.
KEGGiaae:aq_2109.
PATRICi20961068. VBIAquAeo85532_1627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07837.1.
PIRiH70480.
RefSeqiNP_214445.1. NC_000918.1.
WP_010881381.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67876.
SMRiO67876. Positions 9-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2109.

Proteomic databases

PRIDEiO67876.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07837; AAC07837; aq_2109.
GeneIDi1193865.
KEGGiaae:aq_2109.
PATRICi20961068. VBIAquAeo85532_1627.

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
InParanoidiO67876.
KOiK01698.
OMAiSTYQMDP.
OrthoDBiEOG6VXFCB.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.
BioCyciAAEO224324:GJBH-1508-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiHEM2_AQUAE
AccessioniPrimary (citable) accession number: O67876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 29, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.