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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi257 – 2571Manganese 2By similarity
Metal bindingi262 – 2621Manganese 1By similarity
Metal bindingi262 – 2621Manganese 2By similarity
Active sitei269 – 2691Sequence analysis
Metal bindingi280 – 2801Manganese 2By similarity
Metal bindingi339 – 3391Manganese 1By similarity
Metal bindingi341 – 3411Manganese 1By similarity
Metal bindingi341 – 3411Manganese 2By similarity
Active sitei343 – 3431Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1500-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Ordered Locus Names:aq_2099
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Probable cytosol aminopeptidasePRO_0000165717Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_2099.

Structurei

3D structure databases

ProteinModelPortaliO67868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
InParanoidiO67868.
KOiK01255.
OMAiNTPPMIG.
OrthoDBiPOG091H01BT.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEMEVRAFK DDIKKYKGKS VAVFVYEGDL KPLARLSKGT SNKAKRVAEL
60 70 80 90 100
ENFKGKEGEI LKVPTLGTSV DFVYIVGLGK KEKVGEDTYR RASANLVKRM
110 120 130 140 150
RRDKVESTVV VIPRRGDVSK EITKAITEGA ILGNYRFDKY KSKKEDEKFE
160 170 180 190 200
IKEVLINRGD EEGIRLGKIF AEAQNYARNL VNEPGNVINP ITLAEEAKKL
210 220 230 240 250
AEEFGLECKV YDEKQIQEMG MMALYSVGKG SATPPRFIHL IYKPSGKPKE
260 270 280 290 300
KIALVGKGLT FDSGGLNIKP GDYMRTMKMD KSGACAVLGI MRAIAQLKPD
310 320 330 340 350
VEVHGLIGAA ENMPDGNAYR PDDVIKAKNG KYIEIDNTDA EGRVTLADVL
360 370 380 390 400
SYASELKPDK IIDMATLTGA CMVALGEYTA GLFTNAPDFA EEIKKTAKRT
410 420 430 440 450
GERVWELPMD DERLRKKIKN TVADVLNTGG RYGGAITAAM FLEEFVGEGI
460 470 480 490
KWVHLDIAGP AWSKEEYGYY TKGGTGFGVR TCLEYIMKVS SNV
Length:493
Mass (Da):54,543
Last modified:August 1, 1998 - v1
Checksum:iA32B499C7A52065B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07829.1.
PIRiH70479.
RefSeqiNP_214437.1. NC_000918.1.
WP_010881373.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07829; AAC07829; aq_2099.
GeneIDi1193857.
KEGGiaae:aq_2099.
PATRICi20961052. VBIAquAeo85532_1619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07829.1.
PIRiH70479.
RefSeqiNP_214437.1. NC_000918.1.
WP_010881373.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67868.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_2099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07829; AAC07829; aq_2099.
GeneIDi1193857.
KEGGiaae:aq_2099.
PATRICi20961052. VBIAquAeo85532_1619.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
InParanoidiO67868.
KOiK01255.
OMAiNTPPMIG.
OrthoDBiPOG091H01BT.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1500-MONOMER.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_AQUAE
AccessioniPrimary (citable) accession number: O67868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.