O67868 (AMPA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	aq_2099

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165717
Sites
	Active site	269	1	Potential
	Active site	343	1	Potential
	Metal binding	257	1	Manganese 2 By similarity
	Metal binding	262	1	Manganese 1 By similarity
	Metal binding	262	1	Manganese 2 By similarity
	Metal binding	280	1	Manganese 2 By similarity
	Metal binding	339	1	Manganese 1 By similarity
	Metal binding	341	1	Manganese 1 By similarity
	Metal binding	341	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67868 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A32B499C7A52065B	FASTA	493	54,543
	10 20 30 40 50 60 MKEMEVRAFK DDIKKYKGKS VAVFVYEGDL KPLARLSKGT SNKAKRVAEL ENFKGKEGEI 70 80 90 100 110 120 LKVPTLGTSV DFVYIVGLGK KEKVGEDTYR RASANLVKRM RRDKVESTVV VIPRRGDVSK 130 140 150 160 170 180 EITKAITEGA ILGNYRFDKY KSKKEDEKFE IKEVLINRGD EEGIRLGKIF AEAQNYARNL 190 200 210 220 230 240 VNEPGNVINP ITLAEEAKKL AEEFGLECKV YDEKQIQEMG MMALYSVGKG SATPPRFIHL 250 260 270 280 290 300 IYKPSGKPKE KIALVGKGLT FDSGGLNIKP GDYMRTMKMD KSGACAVLGI MRAIAQLKPD 310 320 330 340 350 360 VEVHGLIGAA ENMPDGNAYR PDDVIKAKNG KYIEIDNTDA EGRVTLADVL SYASELKPDK 370 380 390 400 410 420 IIDMATLTGA CMVALGEYTA GLFTNAPDFA EEIKKTAKRT GERVWELPMD DERLRKKIKN 430 440 450 460 470 480 TVADVLNTGG RYGGAITAAM FLEEFVGEGI KWVHLDIAGP AWSKEEYGYY TKGGTGFGVR 490 TCLEYIMKVS SNV

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07829.1.
PIR	H70479.
RefSeq	NP_214437.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67868.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193857.
GenomeReviews	Gene locus aq_2099 in contig AE000657_GR.
KEGG	aae:aq_2099.
NMPDR	fig|224324.1.peg.1452.
PATRIC	20961052. VBIAquAeo85532_1619.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NMHLMRY.
PhylomeDB	O67868.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_2099-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AQUAE

Accession

Primary (citable) accession number: O67868

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program