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O67854 (O67854_AQUAE) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Transporter RuleBase RU003732
Gene names
Name:snf EMBL AAC07817.1
Ordered Locus Names:aq_2077 EMBL AAC07817.1
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP] EMBL AAC07817.1
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Ontologies

Keywords
   Biological processSymport RuleBase RU003732
Transport
   Cellular componentMembrane
   DomainTransmembrane RuleBase RU003732
   LigandChloride PDB 2A65 PDB 3GJD PDB 2QJU PDB 3MPQ PDB 3MPN PDB 3USP PDB 4HOD
Metal-binding PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3TU0 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Sodium PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3TU0 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
   Technical term3D-structure PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3TT3 PDB 3TU0 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

neurotransmitter:sodium symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding201Sodium 1; via carbonyl oxygen PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding221Sodium 2 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM5 PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding231Sodium 1 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding271Sodium 2 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM5 PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding2541Sodium 2 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4HOD
Metal binding2861Sodium 2 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM5 PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding3511Sodium 1 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding3541Sodium 1 PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 PDB 4MM4 PDB 4MM5 PDB 4HMK PDB 4HOD PDB 4MM6 PDB 4MM7 PDB 4MM8 PDB 4MM9 PDB 4MMA PDB 4MMB PDB 4MMC PDB 4MMD PDB 4MME PDB 4MMF
Metal binding3551Sodium 1 PDB 4MM4
Binding site301Imipramine 1 PDB 2Q72
Binding site471Chloride 1 PDB 4HOD
Binding site1651Chloride 2 PDB 3MPQ PDB 3MPN PDB 3USP
Binding site1651Chloride 3 PDB 2A65 PDB 3GJD
Binding site1651Chloride 4 PDB 2QJU
Binding site2501Chloride 1 PDB 4HOD
Binding site4011Imipramine 1 PDB 2Q72

Sequences

Sequence LengthMass (Da)Tools
O67854 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 409A7B590C58D8BA

FASTA51357,408
        10         20         30         40         50         60 
MEVKREHWAT RLGLILAMAG NAVGLGNFLR FPVQAAENGG GAFMIPYIIA FLLVGIPLMW 

        70         80         90        100        110        120 
IEWAMGRYGG AQGHGTTPAI FYLLWRNRFA KILGVFGLWI PLVVAIYYVY IESWTLGFAI 

       130        140        150        160        170        180 
KFLVGLVPEP PPNATDPDSI LRPFKEFLYS YIGVPKGDEP ILKPSLFAYI VFLITMFINV 

       190        200        210        220        230        240 
SILIRGISKG IERFAKIAMP TLFILAVFLV IRVFLLETPN GTAADGLNFL WTPDFEKLKD 

       250        260        270        280        290        300 
PGVWIAAVGQ IFFTLSLGFG AIITYASYVR KDQDIVLSGL TAATLNEKAE VILGGSISIP 

       310        320        330        340        350        360 
AAVAFFGVAN AVAIAKAGAF NLGFITLPAI FSQTAGGTFL GFLWFFLLFF AGLTSSIAIM 

       370        380        390        400        410        420 
QPMIAFLEDE LKLSRKHAVL WTAAIVFFSA HLVMFLNKSL DEMDFWAGTI GVVFFGLTEL 

       430        440        450        460        470        480 
IIFFWIFGAD KAWEEINRGG IIKVPRIYYY VMRYITPAFL AVLLVVWARE YIPKIMEETH 

       490        500        510 
WTVWITRFYI IGLFLFLTFL VFLAERRRNH ESA 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5 EMBL AAC07817.1.
[2]"Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters."
Yamashita A., Singh S.K., Kawate T., Jin Y., Gouaux E.
Nature 437:215-223(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH CHLORIDE AND SODIUM.
[3]"Antidepressant binding site in a bacterial homologue of neurotransmitter transporters."
Singh S.K., Yamashita A., Gouaux E.
Nature 448:952-956(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH IMIPRAMINE AND SODIUM.
[4]"LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake."
Zhou Z., Zhen J., Karpowich N.K., Goetz R.M., Law C.J., Reith M.E., Wang D.N.
Science 317:1390-1393(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 5-513 IN COMPLEX WITH CHLORIDE AND SODIUM.
[5]"A competitive inhibitor traps LeuT in an open-to-out conformation."
Singh S.K., Piscitelli C.L., Yamashita A., Gouaux E.
Science 322:1655-1661(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SODIUM.
[6]"Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures."
Zhou Z., Zhen J., Karpowich N.K., Law C.J., Reith M.E., Wang D.N.
Nat. Struct. Mol. Biol. 16:652-657(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS) IN COMPLEX WITH SODIUM.
[7]"Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation."
Quick M., Winther A.M., Shi L., Nissen P., Weinstein H., Javitch J.A.
Proc. Natl. Acad. Sci. U.S.A. 106:5563-5568(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH CHLORIDE AND SODIUM.
[8]"Structural origins of nitroxide side chain dynamics on membrane protein ?-helical sites."
Kroncke B.M., Horanyi P.S., Columbus L.
Biochemistry 49:10045-10060(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 5-511 IN COMPLEX WITH CHLORIDE AND SODIUM.
[9]"Insights into transport mechanism from LeuT engineered to transport tryptophan."
Piscitelli C.L., Gouaux E.
EMBO J. 31:228-235(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH SODIUM.
[10]"Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue."
Wang H., Gouaux E.
EMBO Rep. 13:861-866(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH SODIUM.
[11]"Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context."
Wang H., Elferich J., Gouaux E.
Nat. Struct. Mol. Biol. 19:212-219(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH CHLORIDE AND SODIUM.
[12]"X-ray structures of LeuT in substrate-free outward-open and apo inward-open states."
Krishnamurthy H., Gouaux E.
Nature 481:469-474(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS) IN COMPLEX WITH SODIUM.
[13]"Structural basis for action by diverse antidepressants on biogenic amine transporters."
Wang H., Goehring A., Wang K.H., Penmatsa A., Ressler R., Gouaux E.
Nature 503:141-145(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH SODIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC07817.1.
PIRB70478.
RefSeqNP_214423.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2A65X-ray1.65A1-513[»]
2Q6HX-ray1.85A1-513[»]
2Q72X-ray1.70A1-513[»]
2QB4X-ray1.90A1-513[»]
2QEIX-ray1.85A1-513[»]
2QJUX-ray2.90A5-513[»]
3F3AX-ray2.00A1-513[»]
3F3CX-ray2.10A1-513[»]
3F3DX-ray2.30A1-513[»]
3F3EX-ray1.80A1-513[»]
3F48X-ray1.90A1-513[»]
3F4IX-ray1.95A1-513[»]
3F4JX-ray2.15A1-513[»]
3GJCX-ray2.80A/B1-513[»]
3GJDX-ray2.00A1-513[»]
3GWUX-ray2.14A1-513[»]
3GWVX-ray2.35A1-513[»]
3GWWX-ray2.46A1-513[»]
3MPNX-ray2.25A5-511[»]
3MPQX-ray2.25A4-510[»]
3QS4X-ray2.63A1-513[»]
3QS5X-ray2.60A1-513[»]
3QS6X-ray2.80A1-513[»]
3TT1X-ray3.10A/B1-513[»]
3TT3X-ray3.22A1-513[»]
3TU0X-ray2.99A1-513[»]
3USGX-ray2.50A1-513[»]
3USIX-ray3.11A/B1-513[»]
3USJX-ray3.50A/B1-513[»]
3USKX-ray4.50A/B/C/D1-513[»]
3USLX-ray2.71A1-513[»]
3USMX-ray3.01A1-513[»]
3USOX-ray4.50A/B1-513[»]
3USPX-ray2.10A1-513[»]
4FXZX-ray2.60A1-513[»]
4FY0X-ray3.00A1-513[»]
4HMKX-ray3.00A/B1-513[»]
4HODX-ray3.30A1-513[»]
4MM4X-ray2.89A/B1-513[»]
4MM5X-ray3.20A1-513[»]
4MM6X-ray3.10A1-513[»]
4MM7X-ray2.85A1-513[»]
4MM8X-ray3.31A1-513[»]
4MM9X-ray2.90A1-513[»]
4MMAX-ray3.30A1-513[»]
4MMBX-ray2.25A1-513[»]
4MMCX-ray2.30A1-513[»]
4MMDX-ray2.30A/B1-513[»]
4MMEX-ray2.50A/B1-513[»]
4MMFX-ray2.70A/B1-513[»]
ProteinModelPortalO67854.
SMRO67854. Positions 3-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_2077.

Protein family/group databases

TCDB2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07817; AAC07817; aq_2077.
GeneID1193843.
KEGGaae:aq_2077.
PATRIC20961014. VBIAquAeo85532_1600.

Phylogenomic databases

HOGENOMHOG000232224.
OMAFSFTGAW.
OrthoDBEOG6WT8F5.
ProtClustDBCLSK2392984.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1486-MONOMER.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 2 hits.
[Graphical view]
PRINTSPR00176. NANEUSMPORT.
PROSITEPS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO67854.

Entry information

Entry nameO67854_AQUAE
AccessionPrimary (citable) accession number: O67854
Entry history
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)