Transporter - Aquifex aeolicus (strain VF5)

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O67854 (O67854_AQUAE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Transporter RuleBase RU003732

Gene names

Name:	snf EMBL AAC07817.1
Ordered Locus Names:	aq_2077 EMBL AAC07817.1

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP] EMBL AAC07817.1

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex ›

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) family. RuleBase RU003732

Ontologies

Keywords
Biological process	Symport RuleBase RU003732 Transport
Cellular component	Membrane
Domain	Transmembrane RuleBase RU003732
Technical term	3D-structure PDB 2Q72 PDB 3QS4 PDB 3F3C PDB 3F3A PDB 3F3E PDB 3F3D PDB 3F4J PDB 3F4I PDB 3F48 PDB 3GWV PDB 3GWU PDB 3GWW PDB 2A65 PDB 3GJC PDB 3GJD PDB 2QJU PDB 2Q6H PDB 2QB4 PDB 2QEI PDB 3MPQ PDB 3MPN PDB 3QS5 PDB 3QS6 PDB 3TT1 PDB 3TT3 PDB 3TU0 PDB 3USG PDB 3USI PDB 3USJ PDB 3USK PDB 3USL PDB 3USM PDB 3USO PDB 3USP PDB 4FXZ PDB 4FY0 Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	neurotransmitter:sodium symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

Imipramine 1 PDB 2Q72

Binding site

401

Imipramine 1 PDB 2Q72

Sequences

Sequence

Length

Mass (Da)

Tools

O67854 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 409A7B590C58D8BA
Show »

FASTA

513

57,408

        10         20         30         40         50         60 
MEVKREHWAT RLGLILAMAG NAVGLGNFLR FPVQAAENGG GAFMIPYIIA FLLVGIPLMW 

        70         80         90        100        110        120 
IEWAMGRYGG AQGHGTTPAI FYLLWRNRFA KILGVFGLWI PLVVAIYYVY IESWTLGFAI 

       130        140        150        160        170        180 
KFLVGLVPEP PPNATDPDSI LRPFKEFLYS YIGVPKGDEP ILKPSLFAYI VFLITMFINV 

       190        200        210        220        230        240 
SILIRGISKG IERFAKIAMP TLFILAVFLV IRVFLLETPN GTAADGLNFL WTPDFEKLKD 

       250        260        270        280        290        300 
PGVWIAAVGQ IFFTLSLGFG AIITYASYVR KDQDIVLSGL TAATLNEKAE VILGGSISIP 

       310        320        330        340        350        360 
AAVAFFGVAN AVAIAKAGAF NLGFITLPAI FSQTAGGTFL GFLWFFLLFF AGLTSSIAIM 

       370        380        390        400        410        420 
QPMIAFLEDE LKLSRKHAVL WTAAIVFFSA HLVMFLNKSL DEMDFWAGTI GVVFFGLTEL 

       430        440        450        460        470        480 
IIFFWIFGAD KAWEEINRGG IIKVPRIYYY VMRYITPAFL AVLLVVWARE YIPKIMEETH 

       490        500        510 
WTVWITRFYI IGLFLFLTFL VFLAERRRNH ESA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5 EMBL AAC07817.1.
[2]	"Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters." Yamashita A., Singh S.K., Kawate T., Jin Y., Gouaux E. Nature 437:215-223(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
[3]	"Antidepressant binding site in a bacterial homologue of neurotransmitter transporters." Singh S.K., Yamashita A., Gouaux E. Nature 448:952-956(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH IMIPRAMINE.
[4]	"LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake." Zhou Z., Zhen J., Karpowich N.K., Goetz R.M., Law C.J., Reith M.E., Wang D.N. Science 317:1390-1393(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 5-513.
[5]	"A competitive inhibitor traps LeuT in an open-to-out conformation." Singh S.K., Piscitelli C.L., Yamashita A., Gouaux E. Science 322:1655-1661(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
[6]	"Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures." Zhou Z., Zhen J., Karpowich N.K., Law C.J., Reith M.E., Wang D.N. Nat. Struct. Mol. Biol. 16:652-657(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS).
[7]	"Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation." Quick M., Winther A.M., Shi L., Nissen P., Weinstein H., Javitch J.A. Proc. Natl. Acad. Sci. U.S.A. 106:5563-5568(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[8]	"Structural origins of nitroxide side chain dynamics on membrane protein ?-helical sites." Kroncke B.M., Horanyi P.S., Columbus L. Biochemistry 49:10045-10060(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 5-511.
[9]	"Insights into transport mechanism from LeuT engineered to transport tryptophan." Piscitelli C.L., Gouaux E. EMBO J. 31:228-235(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
[10]	"Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue." Wang H., Gouaux E. EMBO Rep. 13:861-866(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
[11]	"Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context." Wang H., Elferich J., Gouaux E. Nat. Struct. Mol. Biol. 19:212-219(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).
[12]	"X-ray structures of LeuT in substrate-free outward-open and apo inward-open states." Krishnamurthy H., Gouaux E. Nature 481:469-474(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC07817.1.

PIR

B70478.

RefSeq

NP_214423.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A65	X-ray	1.65	A	1-513	[»]
2Q6H	X-ray	1.85	A	1-513	[»]
2Q72	X-ray	1.70	A	1-513	[»]
2QB4	X-ray	1.90	A	1-513	[»]
2QEI	X-ray	1.85	A	1-513	[»]
2QJU	X-ray	2.90	A	5-513	[»]
3F3A	X-ray	2.00	A	1-513	[»]
3F3C	X-ray	2.10	A	1-513	[»]
3F3D	X-ray	2.30	A	1-513	[»]
3F3E	X-ray	1.80	A	1-513	[»]
3F48	X-ray	1.90	A	1-513	[»]
3F4I	X-ray	1.95	A	1-513	[»]
3F4J	X-ray	2.15	A	1-513	[»]
3GJC	X-ray	2.80	A/B	1-513	[»]
3GJD	X-ray	2.00	A	1-513	[»]
3GWU	X-ray	2.14	A	1-513	[»]
3GWV	X-ray	2.35	A	1-513	[»]
3GWW	X-ray	2.46	A	1-513	[»]
3MPN	X-ray	2.25	A	5-511	[»]
3MPQ	X-ray	2.25	A	4-510	[»]
3QS4	X-ray	2.63	A	1-513	[»]
3QS5	X-ray	2.60	A	1-513	[»]
3QS6	X-ray	2.80	A	1-513	[»]
3TT1	X-ray	3.10	A/B	1-513	[»]
3TT3	X-ray	3.22	A	1-513	[»]
3TU0	X-ray	2.99	A	1-513	[»]
3USG	X-ray	2.50	A	1-513	[»]
3USI	X-ray	3.11	A/B	1-513	[»]
3USJ	X-ray	3.50	A/B	1-513	[»]
3USK	X-ray	4.50	A/B/C/D	1-513	[»]
3USL	X-ray	2.71	A	1-513	[»]
3USM	X-ray	3.01	A	1-513	[»]
3USO	X-ray	4.50	A/B	1-513	[»]
3USP	X-ray	2.10	A	1-513	[»]
4FXZ	X-ray	2.60	A	1-513	[»]
4FY0	X-ray	3.00	A	1-513	[»]

ProteinModelPortal

O67854.

SMR

O67854. Positions 3-513.

ModBase

Search...

Protein-protein interaction databases

STRING

224324.aq_2077.

Protein family/group databases

TCDB

2.A.22.4.2. neurotransmitter:sodium symporter (NSS) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC07817; AAC07817; aq_2077.

GeneID

1193843.

KEGG

aae:aq_2077.

PATRIC

20961014. VBIAquAeo85532_1600.

Phylogenomic databases

HOGENOM

HOG000232224.

OMA

VEVAECA.

ProtClustDB

CLSK2392984.

Enzyme and pathway databases

BioCyc

AAEO224324:GJBH-1486-MONOMER.

Family and domain databases

InterPro

IPR000175. Na/ntran_symport.
[Graphical view]

PANTHER

PTHR11616. PTHR11616. 1 hit.

Pfam

PF00209. SNF. 2 hits.
[Graphical view]

PRINTS

PR00176. NANEUSMPORT.

PROSITE

PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O67854.

Entry information

Entry name

O67854_AQUAE

Accession

Primary (citable) accession number: O67854

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information