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O67854

- O67854_AQUAE

UniProt

O67854 - O67854_AQUAE

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Protein

Transporter

Gene

snf

Organism
Aquifex aeolicus (strain VF5)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Sodium 1; via carbonyl oxygenImported
Metal bindingi22 – 221Sodium 2Imported
Metal bindingi23 – 231Sodium 1Imported
Metal bindingi27 – 271Sodium 2Imported
Binding sitei30 – 301Imipramine 1Imported
Metal bindingi254 – 2541Sodium 2Imported
Metal bindingi286 – 2861Sodium 2Imported
Metal bindingi351 – 3511Sodium 1Imported
Metal bindingi354 – 3541Sodium 1Imported
Metal bindingi355 – 3551Sodium 1Imported
Binding sitei401 – 4011Imipramine 1Imported

GO - Molecular functioni

  1. neurotransmitter:sodium symporter activity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

SymportUniRule annotation, Transport

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1486-MONOMER.

Protein family/group databases

TCDBi2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
TransporterUniRule annotation
Gene namesi
Name:snfImported
Ordered Locus Names:aq_2077Imported
OrganismiAquifex aeolicus (strain VF5)Imported
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_2077.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A65X-ray1.65A1-513[»]
2Q6HX-ray1.85A1-513[»]
2Q72X-ray1.70A1-513[»]
2QB4X-ray1.90A1-513[»]
2QEIX-ray1.85A1-513[»]
2QJUX-ray2.90A5-513[»]
3F3AX-ray2.00A1-513[»]
3F3CX-ray2.10A1-513[»]
3F3DX-ray2.30A1-513[»]
3F3EX-ray1.80A1-513[»]
3F48X-ray1.90A1-513[»]
3F4IX-ray1.95A1-513[»]
3F4JX-ray2.15A1-513[»]
3GJCX-ray2.80A/B1-513[»]
3GJDX-ray2.00A1-513[»]
3GWUX-ray2.14A1-513[»]
3GWVX-ray2.35A1-513[»]
3GWWX-ray2.46A1-513[»]
3MPNX-ray2.25A5-511[»]
3MPQX-ray2.25A4-510[»]
3QS4X-ray2.63A1-513[»]
3QS5X-ray2.60A1-513[»]
3QS6X-ray2.80A1-513[»]
3TT1X-ray3.10A/B1-513[»]
3TT3X-ray3.22A1-513[»]
3TU0X-ray2.99A1-513[»]
3USGX-ray2.50A1-513[»]
3USIX-ray3.11A/B1-513[»]
3USJX-ray3.50A/B1-513[»]
3USKX-ray4.50A/B/C/D1-513[»]
3USLX-ray2.71A1-513[»]
3USMX-ray3.01A1-513[»]
3USOX-ray4.50A/B1-513[»]
3USPX-ray2.10A1-513[»]
4FXZX-ray2.60A1-513[»]
4FY0X-ray3.00A1-513[»]
4HMKX-ray3.00A/B1-513[»]
4HODX-ray3.30A1-513[»]
4MM4X-ray2.89A/B1-513[»]
4MM5X-ray3.20A1-513[»]
4MM6X-ray3.10A1-513[»]
4MM7X-ray2.85A1-513[»]
4MM8X-ray3.31A1-513[»]
4MM9X-ray2.90A1-513[»]
4MMAX-ray3.30A1-513[»]
4MMBX-ray2.25A1-513[»]
4MMCX-ray2.30A1-513[»]
4MMDX-ray2.30A/B1-513[»]
4MMEX-ray2.50A/B1-513[»]
4MMFX-ray2.70A/B1-513[»]
ProteinModelPortaliO67854.
SMRiO67854. Positions 3-513.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67854.

Family & Domainsi

Sequence similaritiesi

Belongs to the sodium:neurotransmitter symporter (SNF) family.UniRule annotation

Keywords - Domaini

TransmembraneUniRule annotation

Phylogenomic databases

HOGENOMiHOG000232224.
InParanoidiO67854.
OMAiMAHSAPK.
OrthoDBiEOG6WT8F5.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 2 hits.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67854 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVKREHWAT RLGLILAMAG NAVGLGNFLR FPVQAAENGG GAFMIPYIIA
60 70 80 90 100
FLLVGIPLMW IEWAMGRYGG AQGHGTTPAI FYLLWRNRFA KILGVFGLWI
110 120 130 140 150
PLVVAIYYVY IESWTLGFAI KFLVGLVPEP PPNATDPDSI LRPFKEFLYS
160 170 180 190 200
YIGVPKGDEP ILKPSLFAYI VFLITMFINV SILIRGISKG IERFAKIAMP
210 220 230 240 250
TLFILAVFLV IRVFLLETPN GTAADGLNFL WTPDFEKLKD PGVWIAAVGQ
260 270 280 290 300
IFFTLSLGFG AIITYASYVR KDQDIVLSGL TAATLNEKAE VILGGSISIP
310 320 330 340 350
AAVAFFGVAN AVAIAKAGAF NLGFITLPAI FSQTAGGTFL GFLWFFLLFF
360 370 380 390 400
AGLTSSIAIM QPMIAFLEDE LKLSRKHAVL WTAAIVFFSA HLVMFLNKSL
410 420 430 440 450
DEMDFWAGTI GVVFFGLTEL IIFFWIFGAD KAWEEINRGG IIKVPRIYYY
460 470 480 490 500
VMRYITPAFL AVLLVVWARE YIPKIMEETH WTVWITRFYI IGLFLFLTFL
510
VFLAERRRNH ESA
Length:513
Mass (Da):57,408
Last modified:August 1, 1998 - v1
Checksum:i409A7B590C58D8BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07817.1.
PIRiB70478.
RefSeqiNP_214423.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07817; AAC07817; aq_2077.
GeneIDi1193843.
KEGGiaae:aq_2077.
PATRICi20961014. VBIAquAeo85532_1600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07817.1 .
PIRi B70478.
RefSeqi NP_214423.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A65 X-ray 1.65 A 1-513 [» ]
2Q6H X-ray 1.85 A 1-513 [» ]
2Q72 X-ray 1.70 A 1-513 [» ]
2QB4 X-ray 1.90 A 1-513 [» ]
2QEI X-ray 1.85 A 1-513 [» ]
2QJU X-ray 2.90 A 5-513 [» ]
3F3A X-ray 2.00 A 1-513 [» ]
3F3C X-ray 2.10 A 1-513 [» ]
3F3D X-ray 2.30 A 1-513 [» ]
3F3E X-ray 1.80 A 1-513 [» ]
3F48 X-ray 1.90 A 1-513 [» ]
3F4I X-ray 1.95 A 1-513 [» ]
3F4J X-ray 2.15 A 1-513 [» ]
3GJC X-ray 2.80 A/B 1-513 [» ]
3GJD X-ray 2.00 A 1-513 [» ]
3GWU X-ray 2.14 A 1-513 [» ]
3GWV X-ray 2.35 A 1-513 [» ]
3GWW X-ray 2.46 A 1-513 [» ]
3MPN X-ray 2.25 A 5-511 [» ]
3MPQ X-ray 2.25 A 4-510 [» ]
3QS4 X-ray 2.63 A 1-513 [» ]
3QS5 X-ray 2.60 A 1-513 [» ]
3QS6 X-ray 2.80 A 1-513 [» ]
3TT1 X-ray 3.10 A/B 1-513 [» ]
3TT3 X-ray 3.22 A 1-513 [» ]
3TU0 X-ray 2.99 A 1-513 [» ]
3USG X-ray 2.50 A 1-513 [» ]
3USI X-ray 3.11 A/B 1-513 [» ]
3USJ X-ray 3.50 A/B 1-513 [» ]
3USK X-ray 4.50 A/B/C/D 1-513 [» ]
3USL X-ray 2.71 A 1-513 [» ]
3USM X-ray 3.01 A 1-513 [» ]
3USO X-ray 4.50 A/B 1-513 [» ]
3USP X-ray 2.10 A 1-513 [» ]
4FXZ X-ray 2.60 A 1-513 [» ]
4FY0 X-ray 3.00 A 1-513 [» ]
4HMK X-ray 3.00 A/B 1-513 [» ]
4HOD X-ray 3.30 A 1-513 [» ]
4MM4 X-ray 2.89 A/B 1-513 [» ]
4MM5 X-ray 3.20 A 1-513 [» ]
4MM6 X-ray 3.10 A 1-513 [» ]
4MM7 X-ray 2.85 A 1-513 [» ]
4MM8 X-ray 3.31 A 1-513 [» ]
4MM9 X-ray 2.90 A 1-513 [» ]
4MMA X-ray 3.30 A 1-513 [» ]
4MMB X-ray 2.25 A 1-513 [» ]
4MMC X-ray 2.30 A 1-513 [» ]
4MMD X-ray 2.30 A/B 1-513 [» ]
4MME X-ray 2.50 A/B 1-513 [» ]
4MMF X-ray 2.70 A/B 1-513 [» ]
ProteinModelPortali O67854.
SMRi O67854. Positions 3-513.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_2077.

Chemistry

DrugBanki DB01242. Clomipramine.
DB01151. Desipramine.
DB00458. Imipramine.

Protein family/group databases

TCDBi 2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07817 ; AAC07817 ; aq_2077 .
GeneIDi 1193843.
KEGGi aae:aq_2077.
PATRICi 20961014. VBIAquAeo85532_1600.

Phylogenomic databases

HOGENOMi HOG000232224.
InParanoidi O67854.
OMAi MAHSAPK.
OrthoDBi EOG6WT8F5.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-1486-MONOMER.

Miscellaneous databases

EvolutionaryTracei O67854.

Family and domain databases

InterProi IPR000175. Na/ntran_symport.
[Graphical view ]
PANTHERi PTHR11616. PTHR11616. 1 hit.
Pfami PF00209. SNF. 2 hits.
[Graphical view ]
PRINTSi PR00176. NANEUSMPORT.
PROSITEi PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5Imported.
  2. "Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters."
    Yamashita A., Singh S.K., Kawate T., Jin Y., Gouaux E.
    Nature 437:215-223(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
  3. "Antidepressant binding site in a bacterial homologue of neurotransmitter transporters."
    Singh S.K., Yamashita A., Gouaux E.
    Nature 448:952-956(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
  4. "LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake."
    Zhou Z., Zhen J., Karpowich N.K., Goetz R.M., Law C.J., Reith M.E., Wang D.N.
    Science 317:1390-1393(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 5-513.
  5. "A competitive inhibitor traps LeuT in an open-to-out conformation."
    Singh S.K., Piscitelli C.L., Yamashita A., Gouaux E.
    Science 322:1655-1661(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
  6. "Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures."
    Zhou Z., Zhen J., Karpowich N.K., Law C.J., Reith M.E., Wang D.N.
    Nat. Struct. Mol. Biol. 16:652-657(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS).
  7. "Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation."
    Quick M., Winther A.M., Shi L., Nissen P., Weinstein H., Javitch J.A.
    Proc. Natl. Acad. Sci. U.S.A. 106:5563-5568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
  8. "Structural origins of nitroxide side chain dynamics on membrane protein ?-helical sites."
    Kroncke B.M., Horanyi P.S., Columbus L.
    Biochemistry 49:10045-10060(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 5-511.
  9. "Insights into transport mechanism from LeuT engineered to transport tryptophan."
    Piscitelli C.L., Gouaux E.
    EMBO J. 31:228-235(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
  10. "Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue."
    Wang H., Gouaux E.
    EMBO Rep. 13:861-866(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
  11. "Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context."
    Wang H., Elferich J., Gouaux E.
    Nat. Struct. Mol. Biol. 19:212-219(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).
  12. "X-ray structures of LeuT in substrate-free outward-open and apo inward-open states."
    Krishnamurthy H., Gouaux E.
    Nature 481:469-474(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS).
  13. "Structural basis for action by diverse antidepressants on biogenic amine transporters."
    Wang H., Goehring A., Wang K.H., Penmatsa A., Ressler R., Gouaux E.
    Nature 503:141-145(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

Entry informationi

Entry nameiO67854_AQUAE
AccessioniPrimary (citable) accession number: O67854
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteomeImported

External Data

Dasty 3