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O67854

- O67854_AQUAE

UniProt

O67854 - O67854_AQUAE

Protein

Transporter

Gene

snf

Organism
Aquifex aeolicus (strain VF5)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi20 – 201Sodium 1; via carbonyl oxygenImported
    Metal bindingi22 – 221Sodium 2Imported
    Metal bindingi23 – 231Sodium 1Imported
    Metal bindingi27 – 271Sodium 2Imported
    Binding sitei30 – 301Imipramine 1Imported
    Metal bindingi254 – 2541Sodium 2Imported
    Metal bindingi286 – 2861Sodium 2Imported
    Metal bindingi351 – 3511Sodium 1Imported
    Metal bindingi354 – 3541Sodium 1Imported
    Metal bindingi355 – 3551Sodium 1Imported
    Binding sitei401 – 4011Imipramine 1Imported

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. neurotransmitter:sodium symporter activity Source: InterPro

    Keywords - Biological processi

    SymportUniRule annotation, Transport

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1486-MONOMER.

    Protein family/group databases

    TCDBi2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TransporterUniRule annotation
    Gene namesi
    Name:snfImported
    Ordered Locus Names:aq_2077Imported
    OrganismiAquifex aeolicus (strain VF5)Imported
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Interactioni

    Protein-protein interaction databases

    STRINGi224324.aq_2077.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2A65X-ray1.65A1-513[»]
    2Q6HX-ray1.85A1-513[»]
    2Q72X-ray1.70A1-513[»]
    2QB4X-ray1.90A1-513[»]
    2QEIX-ray1.85A1-513[»]
    2QJUX-ray2.90A5-513[»]
    3F3AX-ray2.00A1-513[»]
    3F3CX-ray2.10A1-513[»]
    3F3DX-ray2.30A1-513[»]
    3F3EX-ray1.80A1-513[»]
    3F48X-ray1.90A1-513[»]
    3F4IX-ray1.95A1-513[»]
    3F4JX-ray2.15A1-513[»]
    3GJCX-ray2.80A/B1-513[»]
    3GJDX-ray2.00A1-513[»]
    3GWUX-ray2.14A1-513[»]
    3GWVX-ray2.35A1-513[»]
    3GWWX-ray2.46A1-513[»]
    3MPNX-ray2.25A5-511[»]
    3MPQX-ray2.25A4-510[»]
    3QS4X-ray2.63A1-513[»]
    3QS5X-ray2.60A1-513[»]
    3QS6X-ray2.80A1-513[»]
    3TT1X-ray3.10A/B1-513[»]
    3TT3X-ray3.22A1-513[»]
    3TU0X-ray2.99A1-513[»]
    3USGX-ray2.50A1-513[»]
    3USIX-ray3.11A/B1-513[»]
    3USJX-ray3.50A/B1-513[»]
    3USKX-ray4.50A/B/C/D1-513[»]
    3USLX-ray2.71A1-513[»]
    3USMX-ray3.01A1-513[»]
    3USOX-ray4.50A/B1-513[»]
    3USPX-ray2.10A1-513[»]
    4FXZX-ray2.60A1-513[»]
    4FY0X-ray3.00A1-513[»]
    4HMKX-ray3.00A/B1-513[»]
    4HODX-ray3.30A1-513[»]
    4MM4X-ray2.89A/B1-513[»]
    4MM5X-ray3.20A1-513[»]
    4MM6X-ray3.10A1-513[»]
    4MM7X-ray2.85A1-513[»]
    4MM8X-ray3.31A1-513[»]
    4MM9X-ray2.90A1-513[»]
    4MMAX-ray3.30A1-513[»]
    4MMBX-ray2.25A1-513[»]
    4MMCX-ray2.30A1-513[»]
    4MMDX-ray2.30A/B1-513[»]
    4MMEX-ray2.50A/B1-513[»]
    4MMFX-ray2.70A/B1-513[»]
    ProteinModelPortaliO67854.
    SMRiO67854. Positions 3-513.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67854.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sodium:neurotransmitter symporter (SNF) family.UniRule annotation

    Keywords - Domaini

    TransmembraneUniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000232224.
    OMAiMAHSAPK.
    OrthoDBiEOG6WT8F5.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 2 hits.
    [Graphical view]
    PRINTSiPR00176. NANEUSMPORT.
    PROSITEiPS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67854-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVKREHWAT RLGLILAMAG NAVGLGNFLR FPVQAAENGG GAFMIPYIIA    50
    FLLVGIPLMW IEWAMGRYGG AQGHGTTPAI FYLLWRNRFA KILGVFGLWI 100
    PLVVAIYYVY IESWTLGFAI KFLVGLVPEP PPNATDPDSI LRPFKEFLYS 150
    YIGVPKGDEP ILKPSLFAYI VFLITMFINV SILIRGISKG IERFAKIAMP 200
    TLFILAVFLV IRVFLLETPN GTAADGLNFL WTPDFEKLKD PGVWIAAVGQ 250
    IFFTLSLGFG AIITYASYVR KDQDIVLSGL TAATLNEKAE VILGGSISIP 300
    AAVAFFGVAN AVAIAKAGAF NLGFITLPAI FSQTAGGTFL GFLWFFLLFF 350
    AGLTSSIAIM QPMIAFLEDE LKLSRKHAVL WTAAIVFFSA HLVMFLNKSL 400
    DEMDFWAGTI GVVFFGLTEL IIFFWIFGAD KAWEEINRGG IIKVPRIYYY 450
    VMRYITPAFL AVLLVVWARE YIPKIMEETH WTVWITRFYI IGLFLFLTFL 500
    VFLAERRRNH ESA 513
    Length:513
    Mass (Da):57,408
    Last modified:August 1, 1998 - v1
    Checksum:i409A7B590C58D8BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07817.1.
    PIRiB70478.
    RefSeqiNP_214423.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07817; AAC07817; aq_2077.
    GeneIDi1193843.
    KEGGiaae:aq_2077.
    PATRICi20961014. VBIAquAeo85532_1600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07817.1 .
    PIRi B70478.
    RefSeqi NP_214423.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2A65 X-ray 1.65 A 1-513 [» ]
    2Q6H X-ray 1.85 A 1-513 [» ]
    2Q72 X-ray 1.70 A 1-513 [» ]
    2QB4 X-ray 1.90 A 1-513 [» ]
    2QEI X-ray 1.85 A 1-513 [» ]
    2QJU X-ray 2.90 A 5-513 [» ]
    3F3A X-ray 2.00 A 1-513 [» ]
    3F3C X-ray 2.10 A 1-513 [» ]
    3F3D X-ray 2.30 A 1-513 [» ]
    3F3E X-ray 1.80 A 1-513 [» ]
    3F48 X-ray 1.90 A 1-513 [» ]
    3F4I X-ray 1.95 A 1-513 [» ]
    3F4J X-ray 2.15 A 1-513 [» ]
    3GJC X-ray 2.80 A/B 1-513 [» ]
    3GJD X-ray 2.00 A 1-513 [» ]
    3GWU X-ray 2.14 A 1-513 [» ]
    3GWV X-ray 2.35 A 1-513 [» ]
    3GWW X-ray 2.46 A 1-513 [» ]
    3MPN X-ray 2.25 A 5-511 [» ]
    3MPQ X-ray 2.25 A 4-510 [» ]
    3QS4 X-ray 2.63 A 1-513 [» ]
    3QS5 X-ray 2.60 A 1-513 [» ]
    3QS6 X-ray 2.80 A 1-513 [» ]
    3TT1 X-ray 3.10 A/B 1-513 [» ]
    3TT3 X-ray 3.22 A 1-513 [» ]
    3TU0 X-ray 2.99 A 1-513 [» ]
    3USG X-ray 2.50 A 1-513 [» ]
    3USI X-ray 3.11 A/B 1-513 [» ]
    3USJ X-ray 3.50 A/B 1-513 [» ]
    3USK X-ray 4.50 A/B/C/D 1-513 [» ]
    3USL X-ray 2.71 A 1-513 [» ]
    3USM X-ray 3.01 A 1-513 [» ]
    3USO X-ray 4.50 A/B 1-513 [» ]
    3USP X-ray 2.10 A 1-513 [» ]
    4FXZ X-ray 2.60 A 1-513 [» ]
    4FY0 X-ray 3.00 A 1-513 [» ]
    4HMK X-ray 3.00 A/B 1-513 [» ]
    4HOD X-ray 3.30 A 1-513 [» ]
    4MM4 X-ray 2.89 A/B 1-513 [» ]
    4MM5 X-ray 3.20 A 1-513 [» ]
    4MM6 X-ray 3.10 A 1-513 [» ]
    4MM7 X-ray 2.85 A 1-513 [» ]
    4MM8 X-ray 3.31 A 1-513 [» ]
    4MM9 X-ray 2.90 A 1-513 [» ]
    4MMA X-ray 3.30 A 1-513 [» ]
    4MMB X-ray 2.25 A 1-513 [» ]
    4MMC X-ray 2.30 A 1-513 [» ]
    4MMD X-ray 2.30 A/B 1-513 [» ]
    4MME X-ray 2.50 A/B 1-513 [» ]
    4MMF X-ray 2.70 A/B 1-513 [» ]
    ProteinModelPortali O67854.
    SMRi O67854. Positions 3-513.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_2077.

    Chemistry

    DrugBanki DB01242. Clomipramine.
    DB01151. Desipramine.
    DB00458. Imipramine.

    Protein family/group databases

    TCDBi 2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07817 ; AAC07817 ; aq_2077 .
    GeneIDi 1193843.
    KEGGi aae:aq_2077.
    PATRICi 20961014. VBIAquAeo85532_1600.

    Phylogenomic databases

    HOGENOMi HOG000232224.
    OMAi MAHSAPK.
    OrthoDBi EOG6WT8F5.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-1486-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O67854.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 2 hits.
    [Graphical view ]
    PRINTSi PR00176. NANEUSMPORT.
    PROSITEi PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5Imported.
    2. "Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters."
      Yamashita A., Singh S.K., Kawate T., Jin Y., Gouaux E.
      Nature 437:215-223(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
    3. "Antidepressant binding site in a bacterial homologue of neurotransmitter transporters."
      Singh S.K., Yamashita A., Gouaux E.
      Nature 448:952-956(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
    4. "LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake."
      Zhou Z., Zhen J., Karpowich N.K., Goetz R.M., Law C.J., Reith M.E., Wang D.N.
      Science 317:1390-1393(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 5-513.
    5. "A competitive inhibitor traps LeuT in an open-to-out conformation."
      Singh S.K., Piscitelli C.L., Yamashita A., Gouaux E.
      Science 322:1655-1661(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
    6. "Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures."
      Zhou Z., Zhen J., Karpowich N.K., Law C.J., Reith M.E., Wang D.N.
      Nat. Struct. Mol. Biol. 16:652-657(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS).
    7. "Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation."
      Quick M., Winther A.M., Shi L., Nissen P., Weinstein H., Javitch J.A.
      Proc. Natl. Acad. Sci. U.S.A. 106:5563-5568(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
    8. "Structural origins of nitroxide side chain dynamics on membrane protein ?-helical sites."
      Kroncke B.M., Horanyi P.S., Columbus L.
      Biochemistry 49:10045-10060(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 5-511.
    9. "Insights into transport mechanism from LeuT engineered to transport tryptophan."
      Piscitelli C.L., Gouaux E.
      EMBO J. 31:228-235(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
    10. "Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue."
      Wang H., Gouaux E.
      EMBO Rep. 13:861-866(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).
    11. "Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context."
      Wang H., Elferich J., Gouaux E.
      Nat. Struct. Mol. Biol. 19:212-219(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).
    12. "X-ray structures of LeuT in substrate-free outward-open and apo inward-open states."
      Krishnamurthy H., Gouaux E.
      Nature 481:469-474(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS).
    13. "Structural basis for action by diverse antidepressants on biogenic amine transporters."
      Wang H., Goehring A., Wang K.H., Penmatsa A., Ressler R., Gouaux E.
      Nature 503:141-145(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

    Entry informationi

    Entry nameiO67854_AQUAE
    AccessioniPrimary (citable) accession number: O67854
    Entry historyi
    Integrated into UniProtKB/TrEMBL: August 1, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3