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O67854

- O67854_AQUAE

UniProt

O67854 - O67854_AQUAE

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Protein

Transporter

Gene
snf, aq_2077
Organism
Aquifex aeolicus (strain VF5)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Sodium 1; via carbonyl oxygenImported
Metal bindingi22 – 221Sodium 2Imported
Metal bindingi23 – 231Sodium 1Imported
Metal bindingi27 – 271Sodium 2Imported
Binding sitei30 – 301Imipramine 1Imported
Metal bindingi254 – 2541Sodium 2Imported
Metal bindingi286 – 2861Sodium 2Imported
Metal bindingi351 – 3511Sodium 1Imported
Metal bindingi354 – 3541Sodium 1Imported
Metal bindingi355 – 3551Sodium 1Imported
Binding sitei401 – 4011Imipramine 1Imported

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. neurotransmitter:sodium symporter activity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

SymportUniRule annotation, Transport

Keywords - Ligandi

Metal-bindingImported, SodiumImported

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1486-MONOMER.

Protein family/group databases

TCDBi2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
TransporterUniRule annotation
Gene namesi
Name:snfImported
Ordered Locus Names:aq_2077Imported
OrganismiAquifex aeolicus (strain VF5)Imported
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_2077.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A65X-ray1.65A1-513[»]
2Q6HX-ray1.85A1-513[»]
2Q72X-ray1.70A1-513[»]
2QB4X-ray1.90A1-513[»]
2QEIX-ray1.85A1-513[»]
2QJUX-ray2.90A5-513[»]
3F3AX-ray2.00A1-513[»]
3F3CX-ray2.10A1-513[»]
3F3DX-ray2.30A1-513[»]
3F3EX-ray1.80A1-513[»]
3F48X-ray1.90A1-513[»]
3F4IX-ray1.95A1-513[»]
3F4JX-ray2.15A1-513[»]
3GJCX-ray2.80A/B1-513[»]
3GJDX-ray2.00A1-513[»]
3GWUX-ray2.14A1-513[»]
3GWVX-ray2.35A1-513[»]
3GWWX-ray2.46A1-513[»]
3MPNX-ray2.25A5-511[»]
3MPQX-ray2.25A4-510[»]
3QS4X-ray2.63A1-513[»]
3QS5X-ray2.60A1-513[»]
3QS6X-ray2.80A1-513[»]
3TT1X-ray3.10A/B1-513[»]
3TT3X-ray3.22A1-513[»]
3TU0X-ray2.99A1-513[»]
3USGX-ray2.50A1-513[»]
3USIX-ray3.11A/B1-513[»]
3USJX-ray3.50A/B1-513[»]
3USKX-ray4.50A/B/C/D1-513[»]
3USLX-ray2.71A1-513[»]
3USMX-ray3.01A1-513[»]
3USOX-ray4.50A/B1-513[»]
3USPX-ray2.10A1-513[»]
4FXZX-ray2.60A1-513[»]
4FY0X-ray3.00A1-513[»]
4HMKX-ray3.00A/B1-513[»]
4HODX-ray3.30A1-513[»]
4MM4X-ray2.89A/B1-513[»]
4MM5X-ray3.20A1-513[»]
4MM6X-ray3.10A1-513[»]
4MM7X-ray2.85A1-513[»]
4MM8X-ray3.31A1-513[»]
4MM9X-ray2.90A1-513[»]
4MMAX-ray3.30A1-513[»]
4MMBX-ray2.25A1-513[»]
4MMCX-ray2.30A1-513[»]
4MMDX-ray2.30A/B1-513[»]
4MMEX-ray2.50A/B1-513[»]
4MMFX-ray2.70A/B1-513[»]
ProteinModelPortaliO67854.
SMRiO67854. Positions 3-513.

Miscellaneous databases

EvolutionaryTraceiO67854.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

TransmembraneUniRule annotation

Phylogenomic databases

HOGENOMiHOG000232224.
OMAiMAHSAPK.
OrthoDBiEOG6WT8F5.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 2 hits.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67854-1 [UniParc]FASTAAdd to Basket

« Hide

MEVKREHWAT RLGLILAMAG NAVGLGNFLR FPVQAAENGG GAFMIPYIIA    50
FLLVGIPLMW IEWAMGRYGG AQGHGTTPAI FYLLWRNRFA KILGVFGLWI 100
PLVVAIYYVY IESWTLGFAI KFLVGLVPEP PPNATDPDSI LRPFKEFLYS 150
YIGVPKGDEP ILKPSLFAYI VFLITMFINV SILIRGISKG IERFAKIAMP 200
TLFILAVFLV IRVFLLETPN GTAADGLNFL WTPDFEKLKD PGVWIAAVGQ 250
IFFTLSLGFG AIITYASYVR KDQDIVLSGL TAATLNEKAE VILGGSISIP 300
AAVAFFGVAN AVAIAKAGAF NLGFITLPAI FSQTAGGTFL GFLWFFLLFF 350
AGLTSSIAIM QPMIAFLEDE LKLSRKHAVL WTAAIVFFSA HLVMFLNKSL 400
DEMDFWAGTI GVVFFGLTEL IIFFWIFGAD KAWEEINRGG IIKVPRIYYY 450
VMRYITPAFL AVLLVVWARE YIPKIMEETH WTVWITRFYI IGLFLFLTFL 500
VFLAERRRNH ESA 513
Length:513
Mass (Da):57,408
Last modified:August 1, 1998 - v1
Checksum:i409A7B590C58D8BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07817.1.
PIRiB70478.
RefSeqiNP_214423.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07817; AAC07817; aq_2077.
GeneIDi1193843.
KEGGiaae:aq_2077.
PATRICi20961014. VBIAquAeo85532_1600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07817.1 .
PIRi B70478.
RefSeqi NP_214423.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A65 X-ray 1.65 A 1-513 [» ]
2Q6H X-ray 1.85 A 1-513 [» ]
2Q72 X-ray 1.70 A 1-513 [» ]
2QB4 X-ray 1.90 A 1-513 [» ]
2QEI X-ray 1.85 A 1-513 [» ]
2QJU X-ray 2.90 A 5-513 [» ]
3F3A X-ray 2.00 A 1-513 [» ]
3F3C X-ray 2.10 A 1-513 [» ]
3F3D X-ray 2.30 A 1-513 [» ]
3F3E X-ray 1.80 A 1-513 [» ]
3F48 X-ray 1.90 A 1-513 [» ]
3F4I X-ray 1.95 A 1-513 [» ]
3F4J X-ray 2.15 A 1-513 [» ]
3GJC X-ray 2.80 A/B 1-513 [» ]
3GJD X-ray 2.00 A 1-513 [» ]
3GWU X-ray 2.14 A 1-513 [» ]
3GWV X-ray 2.35 A 1-513 [» ]
3GWW X-ray 2.46 A 1-513 [» ]
3MPN X-ray 2.25 A 5-511 [» ]
3MPQ X-ray 2.25 A 4-510 [» ]
3QS4 X-ray 2.63 A 1-513 [» ]
3QS5 X-ray 2.60 A 1-513 [» ]
3QS6 X-ray 2.80 A 1-513 [» ]
3TT1 X-ray 3.10 A/B 1-513 [» ]
3TT3 X-ray 3.22 A 1-513 [» ]
3TU0 X-ray 2.99 A 1-513 [» ]
3USG X-ray 2.50 A 1-513 [» ]
3USI X-ray 3.11 A/B 1-513 [» ]
3USJ X-ray 3.50 A/B 1-513 [» ]
3USK X-ray 4.50 A/B/C/D 1-513 [» ]
3USL X-ray 2.71 A 1-513 [» ]
3USM X-ray 3.01 A 1-513 [» ]
3USO X-ray 4.50 A/B 1-513 [» ]
3USP X-ray 2.10 A 1-513 [» ]
4FXZ X-ray 2.60 A 1-513 [» ]
4FY0 X-ray 3.00 A 1-513 [» ]
4HMK X-ray 3.00 A/B 1-513 [» ]
4HOD X-ray 3.30 A 1-513 [» ]
4MM4 X-ray 2.89 A/B 1-513 [» ]
4MM5 X-ray 3.20 A 1-513 [» ]
4MM6 X-ray 3.10 A 1-513 [» ]
4MM7 X-ray 2.85 A 1-513 [» ]
4MM8 X-ray 3.31 A 1-513 [» ]
4MM9 X-ray 2.90 A 1-513 [» ]
4MMA X-ray 3.30 A 1-513 [» ]
4MMB X-ray 2.25 A 1-513 [» ]
4MMC X-ray 2.30 A 1-513 [» ]
4MMD X-ray 2.30 A/B 1-513 [» ]
4MME X-ray 2.50 A/B 1-513 [» ]
4MMF X-ray 2.70 A/B 1-513 [» ]
ProteinModelPortali O67854.
SMRi O67854. Positions 3-513.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_2077.

Protein family/group databases

TCDBi 2.A.22.4.2. the neurotransmitter:sodium symporter (nss) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07817 ; AAC07817 ; aq_2077 .
GeneIDi 1193843.
KEGGi aae:aq_2077.
PATRICi 20961014. VBIAquAeo85532_1600.

Phylogenomic databases

HOGENOMi HOG000232224.
OMAi MAHSAPK.
OrthoDBi EOG6WT8F5.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-1486-MONOMER.

Miscellaneous databases

EvolutionaryTracei O67854.

Family and domain databases

InterProi IPR000175. Na/ntran_symport.
[Graphical view ]
PANTHERi PTHR11616. PTHR11616. 1 hit.
Pfami PF00209. SNF. 2 hits.
[Graphical view ]
PRINTSi PR00176. NANEUSMPORT.
PROSITEi PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5Imported.
  2. "Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters."
    Yamashita A., Singh S.K., Kawate T., Jin Y., Gouaux E.
    Nature 437:215-223(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH SODIUM.
  3. "Antidepressant binding site in a bacterial homologue of neurotransmitter transporters."
    Singh S.K., Yamashita A., Gouaux E.
    Nature 448:952-956(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH IMIPRAMINE AND SODIUM.
  4. "LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake."
    Zhou Z., Zhen J., Karpowich N.K., Goetz R.M., Law C.J., Reith M.E., Wang D.N.
    Science 317:1390-1393(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 5-513 IN COMPLEX WITH SODIUM.
  5. "A competitive inhibitor traps LeuT in an open-to-out conformation."
    Singh S.K., Piscitelli C.L., Yamashita A., Gouaux E.
    Science 322:1655-1661(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SODIUM.
  6. "Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures."
    Zhou Z., Zhen J., Karpowich N.K., Law C.J., Reith M.E., Wang D.N.
    Nat. Struct. Mol. Biol. 16:652-657(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS) IN COMPLEX WITH SODIUM.
  7. "Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation."
    Quick M., Winther A.M., Shi L., Nissen P., Weinstein H., Javitch J.A.
    Proc. Natl. Acad. Sci. U.S.A. 106:5563-5568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH SODIUM.
  8. "Structural origins of nitroxide side chain dynamics on membrane protein ?-helical sites."
    Kroncke B.M., Horanyi P.S., Columbus L.
    Biochemistry 49:10045-10060(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 5-511 IN COMPLEX WITH SODIUM.
  9. "Insights into transport mechanism from LeuT engineered to transport tryptophan."
    Piscitelli C.L., Gouaux E.
    EMBO J. 31:228-235(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH SODIUM.
  10. "Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue."
    Wang H., Gouaux E.
    EMBO Rep. 13:861-866(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH SODIUM.
  11. "Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context."
    Wang H., Elferich J., Gouaux E.
    Nat. Struct. Mol. Biol. 19:212-219(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH SODIUM.
  12. "X-ray structures of LeuT in substrate-free outward-open and apo inward-open states."
    Krishnamurthy H., Gouaux E.
    Nature 481:469-474(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS) IN COMPLEX WITH SODIUM.
  13. "Structural basis for action by diverse antidepressants on biogenic amine transporters."
    Wang H., Goehring A., Wang K.H., Penmatsa A., Ressler R., Gouaux E.
    Nature 503:141-145(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH SODIUM.

Entry informationi

Entry nameiO67854_AQUAE
AccessioniPrimary (citable) accession number: O67854
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteome

External Data

Dasty 3

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