O67848 (ARGB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	aq_2068

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence AAC07812.1 differs from that shown. Reason: Frameshift at position 9.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112575
Regions
	Region	64 – 65	2	Substrate binding By similarity
Sites
	Binding site	86	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Site	29	1	Transition state stabilizer By similarity
	Site	255	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67848 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: F6A478F4718FABB3	FASTA	298	32,908
	10 20 30 40 50 60 MNELIEKAKV LQEALPYIRE FHGKVFVIKY GGSAMHDEEL RESFARDVVL LKYVGINPVI 70 80 90 100 110 120 VHGGGPQISK TLEKFGIKPK FVGGMRKTDE ETMHVVEMVL SGDINKDIVA LINRYSGEKI 130 140 150 160 170 180 YAVGLSGRDG RLLKAKKLDK ERYFSELGLP VPEEDIGFVG EIVDVNEELI FTLLSHNFIP 190 200 210 220 230 240 VIAPVGVGEE GEAYNVNADL AASEIAGEIK AEKLIYLTDT KGVLDEKGEL ISSLSKDKAE 250 260 270 280 290 ELIKKGVIRE GMIPKVRSAL RALEKGVKKV HIIDGRVKHS ILLEVFTKEG VGTEITLE

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07812.1. Frameshift.
PIR	D70477.
RefSeq	NP_214417.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67848.
SMR	O67848. Positions 7-297.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193837.
GenomeReviews	Gene locus aq_2068 in contig AE000657_GR.
KEGG	aae:aq_2068.
NMPDR	fig|224324.1.peg.1432.
PATRIC	20961002. VBIAquAeo85532_1594.
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	O67848.
ProtClustDB	CLSK2748548.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_2068-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_AQUAE

Accession

Primary (citable) accession number: O67848

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program