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O67820

- IMDH_AQUAE

UniProt

O67820 - IMDH_AQUAE

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei252 – 2521NADUniRule annotation
    Metal bindingi304 – 3041Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi306 – 3061Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei307 – 3071IMPUniRule annotation
    Active sitei309 – 3091Thioimidate intermediateUniRule annotation
    Metal bindingi309 – 3091Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei418 – 4181IMPUniRule annotation
    Metal bindingi472 – 4721Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi473 – 4731Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi474 – 4741Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi302 – 3043NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1450-MONOMER.
    RETL1328306-WGS:GSTH-803-MONOMER.
    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:guaBUniRule annotation
    Ordered Locus Names:aq_2023
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Inosine-5'-monophosphate dehydrogenasePRO_0000093689Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_2023.

    Structurei

    3D structure databases

    ProteinModelPortaliO67820.
    SMRiO67820. Positions 6-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini96 – 15459CBS 1UniRule annotationAdd
    BLAST
    Domaini158 – 21861CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni342 – 3443IMP bindingUniRule annotation
    Regioni365 – 3662IMP bindingUniRule annotation
    Regioni389 – 3935IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiCOG0517.
    HOGENOMiHOG000165754.
    KOiK00088.
    OMAiHGHSKNI.
    OrthoDBiEOG6GTZPV.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67820-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEVEKKIKE GLTFDDVLLV PQYSEVLPHE VDVSTYLTKR IKLNIPIVSA    50
    AMDTVTEARL AIALAREGGI GIIHRNLPIK KQAEEVEKVK KSESGMIINP 100
    VTVKPDTRVK EALDIMAKYK ISGVPVVDEE RKLIGILTNR DLRFIKPEDY 150
    SKPVSEFMTK ENLITAPEGI TLDEAEEIFR KYKIEKLPIV DKEGKIKGLI 200
    TIKDIVKRKK YPNACKDELG RLRVGAAVGT GEETLDRVAA LVEAGVDVIV 250
    VDTAHGHSKR VLETVEKIKA NFPEVDVIAG NVATAEGTKA LIEAGADAVK 300
    VGVGPGSICT TRIVAGVGVP QLTAIMEAAS AAREYDIPII ADGGIRYSGD 350
    IVKALAAGAS AVMLGNLLAG TEEAPGETIY YQGRAYKVYR GMGSLGAMSS 400
    RLSSDRYGQE KMEKFVPEGI EGRVPYKGKL ADVVYQLVGG LRSGMGYVGA 450
    RNIKELQEKA KFVRITWAGY RESHVHDVQI TREAPNYWVD 490
    Length:490
    Mass (Da):53,401
    Last modified:August 1, 1998 - v1
    Checksum:i66605CDDB8348CE4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07779.1.
    PIRiH70473.
    RefSeqiNP_214389.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07779; AAC07779; aq_2023.
    GeneIDi1193717.
    KEGGiaae:aq_2023.
    PATRICi20960942. VBIAquAeo85532_1566.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07779.1 .
    PIRi H70473.
    RefSeqi NP_214389.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67820.
    SMRi O67820. Positions 6-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_2023.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07779 ; AAC07779 ; aq_2023 .
    GeneIDi 1193717.
    KEGGi aae:aq_2023.
    PATRICi 20960942. VBIAquAeo85532_1566.

    Phylogenomic databases

    eggNOGi COG0517.
    HOGENOMi HOG000165754.
    KOi K00088.
    OMAi HGHSKNI.
    OrthoDBi EOG6GTZPV.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .
    BioCyci AAEO224324:GJBH-1450-MONOMER.
    RETL1328306-WGS:GSTH-803-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiIMDH_AQUAE
    AccessioniPrimary (citable) accession number: O67820
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3