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O67820

- IMDH_AQUAE

UniProt

O67820 - IMDH_AQUAE

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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei252 – 2521NADUniRule annotation
Metal bindingi304 – 3041Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi306 – 3061Potassium; via carbonyl oxygenUniRule annotation
Binding sitei307 – 3071IMPUniRule annotation
Active sitei309 – 3091Thioimidate intermediateUniRule annotation
Metal bindingi309 – 3091Potassium; via carbonyl oxygenUniRule annotation
Binding sitei418 – 4181IMPUniRule annotation
Metal bindingi472 – 4721Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi473 – 4731Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi474 – 4741Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi302 – 3043NADUniRule annotation

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1450-MONOMER.
RETL1328306-WGS:GSTH-803-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:aq_2023
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Inosine-5'-monophosphate dehydrogenasePRO_0000093689Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_2023.

Structurei

3D structure databases

ProteinModelPortaliO67820.
SMRiO67820. Positions 6-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 15459CBS 1UniRule annotationAdd
BLAST
Domaini158 – 21861CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni342 – 3443IMP bindingUniRule annotation
Regioni365 – 3662IMP bindingUniRule annotation
Regioni389 – 3935IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
HOGENOMiHOG000165754.
InParanoidiO67820.
KOiK00088.
OMAiHGHSKNI.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67820-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEVEKKIKE GLTFDDVLLV PQYSEVLPHE VDVSTYLTKR IKLNIPIVSA
60 70 80 90 100
AMDTVTEARL AIALAREGGI GIIHRNLPIK KQAEEVEKVK KSESGMIINP
110 120 130 140 150
VTVKPDTRVK EALDIMAKYK ISGVPVVDEE RKLIGILTNR DLRFIKPEDY
160 170 180 190 200
SKPVSEFMTK ENLITAPEGI TLDEAEEIFR KYKIEKLPIV DKEGKIKGLI
210 220 230 240 250
TIKDIVKRKK YPNACKDELG RLRVGAAVGT GEETLDRVAA LVEAGVDVIV
260 270 280 290 300
VDTAHGHSKR VLETVEKIKA NFPEVDVIAG NVATAEGTKA LIEAGADAVK
310 320 330 340 350
VGVGPGSICT TRIVAGVGVP QLTAIMEAAS AAREYDIPII ADGGIRYSGD
360 370 380 390 400
IVKALAAGAS AVMLGNLLAG TEEAPGETIY YQGRAYKVYR GMGSLGAMSS
410 420 430 440 450
RLSSDRYGQE KMEKFVPEGI EGRVPYKGKL ADVVYQLVGG LRSGMGYVGA
460 470 480 490
RNIKELQEKA KFVRITWAGY RESHVHDVQI TREAPNYWVD
Length:490
Mass (Da):53,401
Last modified:August 1, 1998 - v1
Checksum:i66605CDDB8348CE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07779.1.
PIRiH70473.
RefSeqiNP_214389.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07779; AAC07779; aq_2023.
GeneIDi1193717.
KEGGiaae:aq_2023.
PATRICi20960942. VBIAquAeo85532_1566.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07779.1 .
PIRi H70473.
RefSeqi NP_214389.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67820.
SMRi O67820. Positions 6-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_2023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07779 ; AAC07779 ; aq_2023 .
GeneIDi 1193717.
KEGGi aae:aq_2023.
PATRICi 20960942. VBIAquAeo85532_1566.

Phylogenomic databases

eggNOGi COG0517.
HOGENOMi HOG000165754.
InParanoidi O67820.
KOi K00088.
OMAi HGHSKNI.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci AAEO224324:GJBH-1450-MONOMER.
RETL1328306-WGS:GSTH-803-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiIMDH_AQUAE
AccessioniPrimary (citable) accession number: O67820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3